Identification of <italic style="font-style: italic">Illicium difengpi</italic> and Its Fake <italic style="font-style: italic">I. jiadifengpi</italic> Using DNA Barcoding

Xiao-xia YE; Xiao-min WANG; Shan-lan CHEN; Shi-hua ZHAO; Shi-man WU; Ling YANG

doi:10.13422/j.cnki.syfjx.20191511

您当前的位置：

首页 >

文章列表页 >

Identification of Illicium difengpi and Its Fake I. jiadifengpi Using DNA Barcoding

Resource and Quality Evaluation | 更新时间：2021-02-09

- Identification of Illicium difengpi and Its Fake I. jiadifengpi Using DNA Barcoding
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 15, Pages: 185-190(2019)
- 作者机构：
  
  1.玉林师范学院，广西　玉林　 537000；
  2.钦州合浦师范学校，广西　钦州　 535000
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20191511
  CLC： R284.2; R289; R22; R2-031
- Received：02 April 2019，
  
  Published Online：08 April 2019，
  
  Published：05 August 2019
- 稿件说明：
移动端阅览
Xiao-xia YE, Xiao-min WANG, Shan-lan CHEN, et al. Identification of Illicium difengpi and Its Fake I. jiadifengpi Using DNA Barcoding[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(15): 185-190.
DOI：

Xiao-xia YE, Xiao-min WANG, Shan-lan CHEN, et al. Identification of Illicium difengpi and Its Fake I. jiadifengpi Using DNA Barcoding[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(15): 185-190. DOI： 10.13422/j.cnki.syfjx.20191511.

摘要

目的：

评价和比较几个常用DNA条形码候选序列对地枫皮及伪品假地枫皮的鉴定作用。

方法：

采集不同产地的地枫皮及假地枫皮样品，进行总DNA提取，选取核基因内转录间隔区2(ITS2)序列、叶绿体

rbc

L，

mat

K基因序列进行聚合酶链式反应(PCR)扩增，纯化产物测序，利用CondonCode Aligner V3.7.1 校对拼接。

结果：

地枫皮及假地枫皮

rbc

L序列进行多次PCR扩增及测序结果均不理想，初步推测地枫皮及假地枫皮

rbc

L序列太长，进化较慢，不适合作为地枫皮及假地枫皮种间鉴别；地枫皮及假地枫皮的

mat

K基因序列测序成功率分别为0和76.8%，可能是不同类群的植物

mat

K序列的引物标准不一；对地枫皮及假地枫皮的ITS2序列PCR扩增及测序结果最为理想，测序的成功率分别为89.3%及91.2%，对测序结果序列进行分析，地枫皮的ITS2序列总长度均为268个碱基，存在2个变异位点；假地枫皮的ITS2序列总长度均为430个碱基，存在4个或3个变异位点。实验结果说明地枫皮及假地枫皮ITS2序列较短，有明显的变异性，便于扩增，表明ITS2序列用于地枫皮及假地枫皮的分子鉴定优于

rbc

L和

mat

K序列。

结论：

ITS2序列作为标准的DNA条形码能够有效地鉴定地枫皮及其伪品假地枫皮。

Abstract

Objective:

To evaluate and compare the identification of several DNA barcoding candidate sequences on

Illicium difengpi

and its fake

jiadifengpi

Method:

Samples from different origins of

difengpi

and

jiadifengpi

were collect extraction of total DNA

nuclear gene ITS2 sequence

chloroplast

rbc

mat

K gene sequence were selected for PCR amplification

product purification and sequencing

and CondonCode Aligner V3.7.1 was used to proofread stitching.

Result:

PCR amplification and sequencing of

rbc

L sequences of

difengpi

and

jiadifengpi

were not satisfactory. It is assumed that their

rbc

L sequences were too long with slow evolution

which was unsuitable for interbreeding. The success rate of

mat

K sequencing of

difengpi

and

jiadifengpi

was 0 and 76.8%

which may be because primer standards were different for

mat

K sequences of different groups. The results of PCR amplification and sequencing of ITS2 on

difengpi

and

jiadifengpi

were successful

with the success rate of sequencing was 89.3% and 91.2%. Analysis sequencing results

the total length of ITS2 sequences was 268 bases

and there were 2 variation sites of

difengpi

. The total length of ITS2 sequences was 430 bases

and there were 4 or 3 variation sites of

jiadifengpi

. It shows that ITS2 sequences of

difengpi

and

jiadifengpi

were short and has obvious variability and can be amplify

that ITS2 sequence was better than

rbc

L and

mat

K sequence in molecular identification of

difengpi

and

jiadifengpi

Conclusion:

DNA barcoding based on ITS2 sequence was a powerful and efficient tool for identification of

difengpi

and its fake

jiadifengpi

关键词

Keywords

references

王洪禄，何永志，史利利，等．地枫皮研究进展 [J]．实用中医药杂志， 2011 ， 27 ( 8 )： 577 - 578 ．

黄宝优，吴庆华，柯芳．中药地枫皮的研究概况 [J]．大众科技， 2008 ( 1 )： 126，117 ．

刘元，韦焕英，姚树汉，等．地枫皮类药理作用研究 [J]．湖南中医药导报， 1997 ( Z1 )： 72 - 75 ．

唐辉，史艳财，孔德鑫，等．岩溶特有植物地枫皮的种质资源调查及地理分布 [J]．广东农业科学， 2011 ， 38 ( 12 )： 113 - 117 ．

梁惠凌，王满莲，孔德鑫，等．地枫皮及其伪品假地枫皮的研究进展 [J]．广西科学院学报， 2013 ， 29 ( 3 )： 164 - 168 ．

陈瑞生，陈相银，张露露．地枫皮真伪鉴别 [J]．首都医药， 2013 ， 20 ( 17 )： 41 ．

孔德鑫，李雁群，梁惠凌，等．地枫皮营养器官解剖结构特征及其叶片结构的生态适应性 [J]．基因组学与应用生物学， 2012 ， 31 ( 3 )： 282 - 288 ．

孔德鑫，唐辉，韦霄，等．中药地枫皮及其伪品的FTIR分析与鉴定 [J]．光谱实验室， 2010 ， 27 ( 6 )： 2417 - 2421 ．

高秀清，刘敏，李永升．地枫皮与其伪品的鉴别 [J]．时珍国医国药， 2002 ， 13 ( 11 )： 665 - 666 ．

赖茂祥，饶伟源，杨敏，等．地枫皮及其混伪品的生药鉴别 [J]．中药材， 1997 ( 12 )： 601 - 604 ．

梅学伟，刘艳新，门婧，等．地枫皮与伪品地枫皮的UV光谱鉴定 [J]．中医药信息， 1995 ( 3 )： 40 ．

Schmidt G J , Schilling E E . Phylogeny and biogeography of Eupatorium (As terceae: eupat orieae)based on nuclear ITS sequence date [J]. Amer J Bot , 2000 , 87 : 716 - 726 .

CHEN S L , YAO H , HAN J P , et al . Validation of the ITS2 region as a novel DNA bar code for identifying medicinal plant species [J]. PLoS One , 2010 , 5 ( 1 ): e8613 .

任瑶瑶，江南屏，张良，等．峨嵋山区姜科药用植物ITS2序列分析 [J]．中国实验方剂学杂志， 2019 ， 25 ( 1 )： 217 - 223 ．

LI X K , WANG B , HAN R C , et al . Identification of medicinal plant Schisandra chinensis using a potential DNA barcode ITS2 [J]. Acta Soc Bot Pol , 2013 , 82 ( 4 ): 283 - 288 .

张忠廉，宋美芳，李海涛，等．千斤拔属药用植物DNA条形码鉴定研究 [J]．中草药， 2015 ， 46 ( 1 )： 118 - 122 ．

冯杉杉，郑司浩，李亚康，等．中药材威灵仙及其伪品DNA条形码鉴别研究 [J]．药学学报， 2014 ， 49 ( 2 )： 260 - 266 ．

夏至，冯翠元，高致明，等．黄芩及其同属近缘种的DNA条形码鉴定研究 [J]．中草药， 2014 ， 45 ( 1 )： 107 - 112 ．

方强强，王燕，彭春，等．中药DNA条形码分子鉴定技术的应用与展望 [J]．中国实验方剂学杂志， 2018 ， 24 ( 22 )： 197 - 205 ．

王海燕，刘石生，文明富，等．基于matK序列的几种重要大戟科植物系统发育初步研究 [J]．热带作物学报， 2011 ， 32 ( 4 )： 715 - 719 ．

黄琼林，马新业，詹若挺，等．基于rbcL条形码的鸡血藤真伪鉴别 [J]．江苏农业科学， 2016 ， 44 ( 6 )： 57 - 60 ．

夏至，王璐静，李贺敏，等．基于psbA-trnH基因序列对中药材茜草的分子鉴定 [J]．时珍国医国药， 2016 ， 27 ( 2 )： 374 - 376 ．

杨慧洁，杨世海，张淑丽，等．药用植物DNA条形码研究进展 [J]．中草药， 2014 ， 45 ( 18 )： 2581 - 2587 ．

刘义梅．杜鹃花属药用植物DNA条形码的鉴定研究 [D]．武汉：湖北中医药大学， 2011 ．

李保进．叶籽银杏matK和ITS序列分析及系统发育研究 [D]．泰安：山东农业大学， 2008 ．

Kress W J , Wurdack K J , Zimmer E A , et al . Use of DNA barcodes to identify flowering plant [J]. PNAS , 2005 ( 102 ): 8369 - 8374 .

谢伟玲，邹蓉，杨雪，等．珍稀濒危植物DNA条形码研究进展 [J]．北方园艺， 2015 ( 4 )： 178 - 183 ．

费希同，巨苗苗，林源，等． ITS2序列在植物DNA条形码鉴定中的应用(综述) [J]．亚热带植物科学， 2014 ， 43 ( 4 )： 339 - 342 ．

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Identification and Quality Evaluation of Dendrobium flexicaule and Its Related Species

Molecular Identification of Tibetan Codonopsis Plants Based on ITS2 and psbA-trnH Barcode

Molecular Identification of 22 Species of Lauraceae by DNA Barcoding

Identification of 24 Rana Species Including Rana dybowskii and Rana chensinensis Based on COⅠ Sequences

Systematic Identification and Quality Evaluation of Anemonis Flaccidae Rhizoma from 16 Different Places of Origin

Related Author

SUN Ting

YANG Yuzhen

HU Shuxiao

LU Yao

ZHANG Cun

HU Rushan

Fengchao LI

Huimin LI

Related Institution

Nanyang Vocational College of Agriculture

Nanyang Landscaping Administration

Dongfang Hospital， Beijing University of Chinese Medicine

Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences

Sichuan Institute for Drug Control/Key Laboratory of Quality Evaluation of Chinese Patent Medicines，National Medical Products Administration

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰

Identification of Illicium difengpi and Its Fake I. jiadifengpi Using DNA Barcoding

DOI：10.13422/j.cnki.syfjx.20191511

摘要

Abstract

关键词

Keywords

references