Effect of Hydrogen Peroxide (H<sub>2</sub>O<sub>2</sub>) on Content of Total Phenol Flavonoids and Activity of Related Enzymes in <italic style="font-style: italic">Polygala tenuifolia</italic> Callus

Fu-lin CAO; Ben-xiang HU; Liang PENG; Lu LUO; Jie LI; Ting ZHAO; Yan-ru AN; Tao HUANG

doi:10.13422/j.cnki.syfjx.20191515

您当前的位置：

首页 >

文章列表页 >

Effect of Hydrogen Peroxide (H₂O₂) on Content of Total Phenol Flavonoids and Activity of Related Enzymes in Polygala tenuifolia Callus

Resource and Quality Evaluation | 更新时间：2021-02-09

- Effect of Hydrogen Peroxide (H₂O₂) on Content of Total Phenol Flavonoids and Activity of Related Enzymes in Polygala tenuifolia Callus
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 20, Pages: 153-159(2019)
- 作者机构：
  
  陕西中医药大学，陕西　咸阳　 712046
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20191515
  CLC： R284.2; R289; R22; R2-031
- Received：13 November 2018，
  
  Published Online：19 April 2019，
  
  Published：20 October 2019
- 稿件说明：
移动端阅览
Fu-lin CAO, Ben-xiang HU, Liang PENG, et al. Effect of Hydrogen Peroxide (H₂O₂) on Content of Total Phenol Flavonoids and Activity of Related Enzymes in Polygala tenuifolia Callus[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(20): 153-159.
DOI：

Fu-lin CAO, Ben-xiang HU, Liang PENG, et al. Effect of Hydrogen Peroxide (H₂O₂) on Content of Total Phenol Flavonoids and Activity of Related Enzymes in Polygala tenuifolia Callus[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(20): 153-159. DOI： 10.13422/j.cnki.syfjx.20191515.

摘要

目的：

研究远志愈伤组织在加有不同浓度H

的MS培养基上总酚总黄酮含量变化及抗氧化酶活性变化，从细胞水平探讨其适应H

环境胁迫的生理机制。

方法：

以远志愈伤组织为实验材料，H

浓度设置为0，5，10，15，20 mmol·L

－1

5个梯度加至MS培养基中，分别在培养5，10，15，20，25 d后取样测定愈伤组织总酚、总黄酮含量及抗氧化酶活性。

结果：

浓度在5 mmol·L

－1

下远志愈伤组织15 d时总酚、总黄酮含量最高。当远志愈伤组织培养5 d，外源H

浓度为5 mmol·L

－1

时的超氧化物歧化酶(SOD)活性最高。培养25 d，外源H

浓度为5 mmol·L

－1

时的过氧化物酶(POD)活性最高。培养25 d，外源H

浓度为15 mmol·L

－1

时的过氧化氢酶(CAT)活性最高。

结论：

远志愈伤组织具有适应一定浓度H

环境胁迫的能力，在其受到H

环境胁迫时，远志愈伤组织能通过调节自身的次生代谢物、保护酶系统来缓解带来的伤害。对次生代谢物总酚、总黄酮的含量在5～10 mmol·L

－1

时有显著促进作用；在5 d，外源H

浓度为5 mmol·L

－1

时可显著提高SOD活性；在25 d，外源H

浓度为5 mmol·L

－1

时可显著提高POD活性；在25 d，外源H

浓度为15 mmol·L

－1

时可显著提高CAT活性。

Abstract

Objective:

To study the changes in total phenolic and flavonoid content and antioxidant enzyme activity of

Polygala tenuifolia

callus in MS medium with different concentrations of H

in order to explore the physiological mechanism of

Polygala tenuifolia

callus in adapting to H

environmental stress at the cellular level.

Method:

Five gradients of 0

20 mmol·L

-1

were set for H

concentration and added to MS medium

with

tenuifolia

callus as the experimental material. Total phenols

total flavonoids and antioxidant enzyme activities of callus were determined after 5

25 d of culture

respectively.

Result:

The contents of total phenols and flavonoids were the highest when the concentration of H

was 5 mmol·L

-1

for 15 d. The SOD activity was the highest when the callus was cultured for 5 d and the exogenous H

concentration was 5 mmol·L

-1

. POD activity was the highest at 25 d and 5 mmol·L

-1

concentration of exogenous H

.CAT activity was the highest at 25 d and 15 mmol·L

-1

concentration of exogenous H

Conclusion:

tenuifolia

callus has the ability to adapt to the environmental stress of H

at a certain concentration. When it is subjected to the environmental stress of H

tenuifolia

callus can alleviate the damage by regulating its secondary metabolites and protecting enzyme system. It can significantly promote the content of total phenols and flavonoids in secondary metabolites at 5-10 mmol·L

-1

. SOD activity was significantly increased at 5 d and the concentration of exogenous H

of 5 mmol·L

-1

. POD activity was significantly increased at 25 d and the concentration of exogenous H

of 5 mmol·L

-1

. CAT activity was significantly increased at 25 d and concentration of exogenous H

of 15 mmol·L

-1

关键词

Keywords

references

国家药典委员会．中华人民共和国药典．一部 [M]．北京：中国医药科技出版社， 2015 ： 156 - 157 ．

滕红梅，张书锋，胡正海，等．远志主产区药材中远志皂苷元和多糖量的比较 [J]．中草药， 2009 ， 40 ( 7 )： 1143 - 1146 ．

彭亮，杨冰月，王媛媛，等．远志种苗的质量分级标准 [J]．中国实验方剂学杂志， 2018 ， 24 ( 17 )： 54 - 59 ．

张陶珍，荣巍巍，李清，等．远志的研究进展 [J]．中草药， 2016 ， 47 ( 13 )： 2381 - 2389 ．

黄涛，安衍茹，彭亮，等．外源激素处理对远志种子萌发及幼苗生长的影响 [J]．中国实验方剂学杂志， 2018 ， 24 ( 20 )： 50 - 55 ．

许丁帆，李建科，刘艳军，等．铁皮石斛愈伤组织稳定培养体系的建立 [J]．天津农业科学， 2018 ， 24 ( 8 )： 67-69，90 ．

邓云云，张俊娥． H2O2对杜仲愈伤组织中黄酮含量及过氧化物酶活性的影响 [J]．分子植物育种， 2018 ， 16 ( 3 )： 1007 - 1010 ．

徐茂军，董菊芳．一氧化氮通过依赖和不依赖活性氧的信号途径介导桔青霉细胞壁诱导子促进红豆杉悬浮细胞中紫杉醇生物合成 [J]．科学通报， 2006 ， 14 ： 1675 - 1682 ．

杨芳，张宗申，杨斌．过氧化氢对丹参悬浮细胞生长及次生代谢物积累的影响 [J]．西南农业学报， 2011 ， 24 ( 3 )： 901 - 904 ．

许丁帆，李建科，刘艳军，等．铁皮石斛愈伤组织稳定培养体系的建立 [J]．天津农业科学， 2018 ， 24 ( 8 )： 67-69，90 ．

彭亮，杨冰月，程虎印，等．不同干燥方法对远志筒及根中主要化学成分的影响 [J]．中草药， 2018 ， 49 ( 21 )： 5010 - 5017 ．

巴寅颖，姜艳艳，刘洋等．基于远志酮在记忆障碍模型大鼠体内药代动力学特性的远志及开心散药物属性研究 [J]．北京中医药大学学报， 2012 ， 35 ( 8 )： 549 - 553 ．

彭亮，杨冰月，张琳，等．野生与栽培远志HPLC指纹图谱及化学模式识别研究 [J]．中草药， 2018 ， 49 ( 21 )： 4998 - 5003 ．

王丹丹，白璐，许晓双，等．从农艺性状及化学成分测定分析远志药材商品品规和良种选育的合理性 [J]．中国中药杂志， 2016 ， 41 ( 20 )： 3733 - 3740 ．

张志斌，颜日明，邱晓芳，等．杜仲愈伤组织与悬浮细胞中黄酮和绿原酸积累研究 [J]．中国中药杂志， 2009 ， 34 ( 13 )： 1636 - 1639 ．

周云凯，李伟平，田莎莎，等．白及须根和块茎总酚含量的测定 [J]．中国实验方剂学杂志， 2012 ， 18 ( 10 )： 161 - 164 ．

王学奎．植物生理生化实验原理和技术 [M]．北京：高等教育出版社， 2005 ．

陈颖，杨华，乐利，等．过氧化氢对银杏悬浮细胞黄酮含量及抗氧化酶活性的影响 [J]．中南林业科技大学学报， 2014 ， 34 ( 12 )： 40-45，55 ．

Pei Z M , Murata Y , Benning G , et al . Calcium channels activated by hydrogen peroxide mediate abscisic signaling in guardcells [J]. Nature , 2000 , 406 ( 6797 ): 731 - 734 .

马廷蕊，张金文，梁慧光，等．外源NO和H2O2诱导不同颜色马铃薯花青素积累 [J]．中国蔬菜， 2012 ( 16 )： 63 - 67 ．

彭亮，赵停，杨冰月，等．不同光质光强对远志生长和相关酶活性及成分的影响 [J]．中草药， 2018 ， 49 ( 21 )： 5004 - 5009 ．

何士敏，白珍明，秦家顺． H2O2促进大豆种子萌发的研究 [J]．种子， 2007 ， 26 ( 8 )： 32 - 35 ．

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Effect of Different Hormone Ratios on Callus Induction and Flavone Accumulation in Polygala tenuifolia

Structure-activity Omics on Anti-inflammatory and Analgesic Effect of Glycyrrhizae Radix et Rhizoma in Qizhiweitong Granules

Structure-activity Omics on Anti-inflammatory and Analgesic Effect of Cyperi Rhizoma in Qizhi Weitong Granules

Analysis of Chemical Constituents in Puerariae Lobatae Radix Dispensing Granules by UPLC-Q-TOF-MS/MS

Effect of H₂O₂ on Secondary Metabolism in Atractylodes chinensis and Its Mechanism

Related Author

Jie LI

Ben-xiang HU

Liang PENG

Bing-yue YANG

Lu LUO

Fu-lin CAO

Ting ZHAO

Yan-ru AN

Related Institution

Shaanxi University of Chinese Medicine

China Resources Sanjiu Medical & Pharmaceutical Co. Ltd.

Liaoning Province Modern TCM Research and Engineering Laboratory

Liaoning Multi-dimensional Analysis of TCM Technical Innovation Center

College of Pharmacy， Liaoning University of Traditional Chinese Medicine （TCM）

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰

Effect of Hydrogen Peroxide (H2O2) on Content of Total Phenol Flavonoids and Activity of Related Enzymes in Polygala tenuifolia Callus

DOI：10.13422/j.cnki.syfjx.20191515

摘要

Abstract

关键词

Keywords

references

Effect of Hydrogen Peroxide (H₂O₂) on Content of Total Phenol Flavonoids and Activity of Related Enzymes in Polygala tenuifolia Callus