Identification of Salviae Yunnanensis Radix et Rhizoma and Its Allied Species Based on Chemical Composition by FTIR with Chemometric Analysis and HPLC Fingerprint

Jian-yong HU; Ming-jin MIAO; Kun WEN; Li XU; Bao-zhong DUAN

doi:10.13422/j.cnki.syfjx.20191519

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Identification of Salviae Yunnanensis Radix et Rhizoma and Its Allied Species Based on Chemical Composition by FTIR with Chemometric Analysis and HPLC Fingerprint

Topic of Research on Medicinal Materials Resources in Yunnan | 更新时间：2021-02-09

- Identification of Salviae Yunnanensis Radix et Rhizoma and Its Allied Species Based on Chemical Composition by FTIR with Chemometric Analysis and HPLC Fingerprint
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 15, Pages: 8-14(2019)
- 作者机构：
  
  1.大理大学　药学与化学学院，云南　大理　 671000；
  2.大理州食品药品检验所，云南　大理　 671000；
  3.大理大学　基础医学院，云南　大理　 671000
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20191519
  CLC： R284.1; R289; R22; R2-031
- Received：13 March 2019，
  
  Published Online：24 April 2019，
  
  Published：05 August 2019
- 稿件说明：
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Jian-yong HU, Ming-jin MIAO, Kun WEN, et al. Identification of Salviae Yunnanensis Radix et Rhizoma and Its Allied Species Based on Chemical Composition by FTIR with Chemometric Analysis and HPLC Fingerprint[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(15): 8-14.
DOI：

Jian-yong HU, Ming-jin MIAO, Kun WEN, et al. Identification of Salviae Yunnanensis Radix et Rhizoma and Its Allied Species Based on Chemical Composition by FTIR with Chemometric Analysis and HPLC Fingerprint[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(15): 8-14. DOI： 10.13422/j.cnki.syfjx.20191519.

摘要

目的：

探讨民族药紫丹参及其近缘种化学成分异同，为紫丹参药材鉴别和临床应用提供参考。

方法：

利用傅里叶红外光谱结合主成分分析(PCA)，聚类分析(HCA)和偏最小二乘法判别分析(PLS-DA)方法对紫丹参及其近缘种成分的差异进行了研究，同时采用高效液相色谱法比较紫丹参及近缘种的水溶性成分差异。

结果：

紫丹参与其近缘种的红外光谱和水溶性HPLC色谱在吸收峰的峰数、峰强、峰形方面均存在一定差异，尤其是其水溶性极性成分较其他品种更为丰富，表明紫丹参与其近缘种之间所含化学成分有相似之处，但亦存在明显的不同。从化学亲缘关系来看，紫丹参与其他同属药物较远，三叶鼠尾草、戟叶鼠尾草、甘西鼠尾草三者成分更为接近；红外光谱结合化学计量学方法PCA，HCA和PLS-DA分析显示，紫丹参与其他品种可明显分开。

结论：

该研究建立的红外光谱结合化学计量学模型，可用于紫丹参及其近缘种的快速鉴别；与其近缘种相比，紫丹参具有独特的化学成分群，具有深入开发的价值，为紫丹参及其混伪品的鉴别提供科学参考。

Abstract

Objective:

To investigate the differences of chemical constituents of Salviae Yunnanensis Radix et Rhizoma and its closely related species

and to provide reference for the clinical application of Salviae Yunnanensis Radix et Rhizoma.

Method:

Fourier transform infrared (FTIR) spectroscopy combined with principal component analysis (PCA)

cluster analysis (HCA) and partial least squares discriminant analysis (PLS-DA) were used to study the differences of components between Salviae Yunnanensis Radix et Rhizoma and its closely related species

and high performance liquid chromatography (HPLC) was used to compare the differences of water-soluble components between them.

Result:

There were some differences between Salviae Yunnanensis Radix et Rhizoma and its closely related species acrcording to FTIR spectroscopy and HPLC fingerprint

especially the water-soluble polar components were more abundant in Salviae Yunnanensis Radix et Rhizoma than other species. The chemical components of

Salvia trijuga

przewalskii

and

bulleyana

were more similar in terms of their genetic relationship. The result showed that the Salviae Yunnanensis Radix et Rhizoma and its closely related species can be clearly distinguished by FTIR combined with chemometrics method.

Conclusion:

Compared with its closely related species

Salviae Yunnanensis Radix et Rhizoma has a unique chemical composition

which has great therapeutic potential for blood stasis. The FTIR combined with chemometrics model can be used for rapid identification of Salviae Yunnanensis Radix et Rhizoma and its closely related species.

关键词

Keywords

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