Exploring Potential Mechanism of " Fu Zheng" Rule of Ulcerative Colitis Based on Network Pharmacology

Bing-yu CHEN; Ke LIU; Fei GE; Zhi-guang SUN

doi:10.13422/j.cnki.syfjx.20191541

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Exploring Potential Mechanism of " Fu Zheng" Rule of Ulcerative Colitis Based on Network Pharmacology

Data Mining | 更新时间：2021-02-09

- Exploring Potential Mechanism of " Fu Zheng" Rule of Ulcerative Colitis Based on Network Pharmacology
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 15, Pages: 191-196(2019)
- 作者机构：
  
  1.南京中医药大学　第二附属医院，南京　 210000；
  2.郑州市人民医院，郑州　 450000；
  3.海安市中医院，江苏　海安　 226600
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20191541
  CLC： R2-0; R318.14
- Received：26 March 2019，
  
  Published Online：19 April 2019，
  
  Published：05 August 2019
- 稿件说明：
移动端阅览
Bing-yu CHEN, Ke LIU, Fei GE, et al. Exploring Potential Mechanism of " Fu Zheng" Rule of Ulcerative Colitis Based on Network Pharmacology[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(15): 191-196.
DOI：

Bing-yu CHEN, Ke LIU, Fei GE, et al. Exploring Potential Mechanism of " Fu Zheng" Rule of Ulcerative Colitis Based on Network Pharmacology[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(15): 191-196. DOI： 10.13422/j.cnki.syfjx.20191541.

摘要

目的：

通过数据库检索与网络药理学方法探讨溃疡性结肠炎 “扶正”治则的潜在机制。

方法：

从中医E百数据库检索溃疡性结肠炎相关方剂，检索方式为“主治肠澼/久痢/痢疾”，筛选所检索到的处方并进行药物种类、用药频次统计分析，选择溃疡性结肠炎治疗的常用扶正药物。将选择后的扶正药物输入中药系统药理学分析平台检索所有化学成分和作用靶点，构建化合物-靶点相互作用网络图；通过Digsee数据库筛选溃疡性结肠炎相关靶点，构建疾病-靶点相互作用网络图；筛选两个网络图的核心靶点，利用DAVID工具对核心靶点进行富集分析。

结果：

数据库共检索到溃疡性结肠炎相关方剂731首，根据纳入、排除标准筛选后获得处方304首，中药220味，经用药频次统计后，以甘草、人参、白术为溃疡性结肠炎扶正治则的代表药物。基于药代动力学ADME参数对上述药物的化学成分进行筛选和靶标预测，共得到136个蛋白靶点；将化合物-靶点、疾病-靶点的蛋白质—蛋白质相互作用网络图合并，以“节点连接度”“节点介度”和“节点紧密度”为评价标准，筛选共有靶点129个；对入选靶标进行京都基因与基因组百科全书(KEGG)富集分析，以P值筛选出前20条通路在溃疡性结肠炎方面具有作用。

结论：

扶正药物治疗溃疡性结肠炎作用的发挥是多靶点、多通路的整体效应，挖掘了溃疡性结肠炎扶正治则的潜在机制，为进一步实验研究提供了理论基础。

Abstract

Objective:

Through database search and network pharmacology to explore the potential mechanism of " Fu Zheng" rule of ulcerative colitis.

Method:

Search for ulcerative colitis related prescriptions from the Chinese medicine E database

Screening the retrieved prescriptions and conducting statistical analysis of the types and frequency of drugs

and selecting the objective drugs for the treatment of ulcerative colitis. Search for the chemical composition and target of drugs on the Digsee platform

then constructing a compound-target interaction network map. Screening for ulcerative colitis related targets through the Digsee database and construct a disease-target interaction network map. Filter the core targets of the two network maps and using the DAVID tool to perform enrichment analysis on the core targets.

Result:

About 731 prescriptions of ulcerative colitis were retrieved from the database

304 prescriptions and 220 Chinese medicines were received based on the inclusion and exclusion criteria. Representative drugs of " Fu Zheng" Rule of ulcerative colitis is Glycyrrhizae Radix et Rhizoma

Ginseng Radix et Rhizoma

Atractylodis Macrocephalae Rhizoma. Screening for chemical constituents of the drug based on ADME parameters

and then target prediction

we received a total of 137 protein targets. Merge the compound-target interaction network map and the disease-target interaction network map

with " Degree" " betweenness " and " closeness" as the evaluation criteria

we found 129 common targets. Perform KEGG enrichment analysis on the selected targets

find out the top 20 pathways with P values has an effect on ulcerative colitis.

Conclusion:

The role of FuZheng Chinese medicine in the treatment of ulcerative colitis is the overall effect of multiple targets and multiple pathways

excavating the potential mechanism of " Fu Zheng" rule of ulcerative colitis and provide a theoretical basis for further experimental research.

关键词

Keywords

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