Metabonomics Analysis of Sailuotong Capsules in Treatment of Acute Cerebral Ischemia in Rats

Cong ZHANG; Jian-xun LIU; Ming-qian SUN; Ye-hao ZHANG; Chang-ying REN; Chun-yuan YIN

doi:10.13422/j.cnki.syfjx.20191552

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Metabonomics Analysis of Sailuotong Capsules in Treatment of Acute Cerebral Ischemia in Rats

Drug Metabolism | 更新时间：2021-02-09

- Metabonomics Analysis of Sailuotong Capsules in Treatment of Acute Cerebral Ischemia in Rats
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 14, Pages: 136-141(2019)
- 作者机构：
  
  1.中国中医科学院　西苑医院　基础医学研究所，中药药理北京市重点实验室，北京　 100091；
  2.北京中医药大学，北京　 100029
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20191552
  CLC： R22; R969.1; R743; O657; R28; C37
- Received：10 January 2019，
  
  Published Online：22 April 2019，
  
  Published：20 July 2019
- 稿件说明：
移动端阅览
Cong ZHANG, Jian-xun LIU, Ming-qian SUN, et al. Metabonomics Analysis of Sailuotong Capsules in Treatment of Acute Cerebral Ischemia in Rats[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(14): 136-141.
DOI：

Cong ZHANG, Jian-xun LIU, Ming-qian SUN, et al. Metabonomics Analysis of Sailuotong Capsules in Treatment of Acute Cerebral Ischemia in Rats[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(14): 136-141. DOI： 10.13422/j.cnki.syfjx.20191552.

摘要

目的：

从代谢组学角度研究塞络通胶囊治疗急性多发性脑梗死大鼠模型的作用机制。

方法：

24只SD大鼠随机分为假手术组、模型组和塞络通组(33 mg·kg

－1

)，通过大鼠颈内动脉注射荧光微球的方法建立急性多发性脑梗死大鼠模型。手术成功后，塞络通组大鼠通过十二指肠注射给药，给药体积2 mL·kg

－1

。基于UPLC-Q-TOF-MS分析各组大鼠脑组织中内源性代谢物，应用主成分分析(PCA)和偏最小二乘法-判别分析(PLS-DA)进行模式识别及生物标志物的鉴定。

结果：

通过模式识别发现假手术组与模型组的代谢轮廓有明显差异，并鉴定了10个与急性脑缺血相关的生物标志物。与假手术组相比，模型组中

-乙酰天门冬氨酸(NAA)，延胡索酸，谷胱甘肽，脱氢抗坏血酸，天门冬氨酸以及

-腺苷同型半胱氨酸含量下降，精氨酸、瓜氨酸、酵母氨酸、乙内酰脲-5-丙酸含量升高；而塞络通胶囊则可使上述10个异常变化的生物标志物水平显著回调。

结论：

塞络通胶囊主要调节的代谢途径有NAA代谢、精氨酸代谢、能量代谢及氧化应激等。

Abstract

Objective:

To explore the mechanism of Sailuotong capsules in treating acute cerebral ischemia from the perspective of metabonomics.

Method:

A total of 24 SD rats were randomly divided into 3 groups

including sham-operated group

model group and Sailuotong group (33 mg·kg

-1

). The rat model of acute multiple cerebral infarction was established by injecting fluorescent microspheres into internal carotid artery. After the successful operation

rats in Sailuotong group were administered by duodenal injection immediately

and the dosage volume was 2 mL·kg

-1

. Endogenous metabolites in rat brain tissues of each group were determined by UPLC-Q-TOF-MS. The relevant data and biomarkers were analyzed by principal component analysis (PCA) and partial least squares-discriminant analysis (PLS-DA).

Result:

The analysis of pattern recognition indicated that the metabolite profiles in model group and sham-operated group were separated obviously

and ten biomarkers related to acute cerebral ischemia were also identified. Compared with the sham-operated group

contents of

-acetylaspartate (NAA)

fumaric acid

glutathione

dehydroascorbic acid

aspartic acid and

-adenosylhomocysteine were decreased

while the contents of arginine

citrulline

saccharopine and hydantoin-5-propionic acid were increased in the model group. Meanwhile

the ten abnormal biomarkers mentioned above got restoration in Sailuotong group.

Conclusion:

The main regulated metabolic pathways of Sailuotong capsules are NAA metabolism

arginine metabolism

energy metabolism

oxidative stress

etc.

关键词

Keywords

references

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