Intestinal Microflora Diversity of Rats Treated with Scutellariae Radix Based on Theory of " Insight into Abdomen"

Hong-yu LIU; Ya-nan ZHANG; Zi-hui LI; Ping-ping CHEN; Hai-xue KUANG; Shu-min LIU

doi:10.13422/j.cnki.syfjx.20191605

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Intestinal Microflora Diversity of Rats Treated with Scutellariae Radix Based on Theory of " Insight into Abdomen"

Academic Discussion | 更新时间：2021-02-09

- Intestinal Microflora Diversity of Rats Treated with Scutellariae Radix Based on Theory of " Insight into Abdomen"
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 18, Pages: 167-173(2019)
- 作者机构：
  
  黑龙江中医药大学　北药基础与应用研究教育部重点实验室，黑龙江省中药天然药物药效物质基础研究重点实验室，药物安全性评价中心，中医药研究院，哈尔滨　 150040
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20191605
  CLC： R2-0; R22; R285.5; R289
- Received：06 January 2019，
  
  Published Online：07 May 2019，
  
  Published：20 September 2019
- 稿件说明：
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Hong-yu LIU, Ya-nan ZHANG, Zi-hui LI, et al. Intestinal Microflora Diversity of Rats Treated with Scutellariae Radix Based on Theory of " Insight into Abdomen"[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(18): 167-173.
DOI：

Hong-yu LIU, Ya-nan ZHANG, Zi-hui LI, et al. Intestinal Microflora Diversity of Rats Treated with Scutellariae Radix Based on Theory of " Insight into Abdomen"[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(18): 167-173. DOI： 10.13422/j.cnki.syfjx.20191605.

摘要

目的：

探讨黄芩对生理状态下大鼠肠道菌群多样性的变化，分析并判定黄芩药性的归属。

方法：

利用16S rRNA高通量基因测序技术对黄芩给药(10 g·kg

－1

)干预后的大鼠盲肠内溶物进行检测，将肠道菌群分类单元(OTUs)数量，丰富度与多样性指数及差异菌门与菌属进行综合性分析，并利用网络可视化手段找寻差异菌门与菌属之间的关联性。

结果：

基于Illumina Miseq平台，与空白组相比，黄芩给药组大鼠肠道菌群的OTUs数量减少，丰富度与多样性指数降低；拟杆菌门(Bacteroidetes)和螺旋体菌门(Spirochaetes)显著性上调(

0.01)，厚壁菌门(Firmicutes)，放线菌门(Actinobacteria)，变形菌门(Proteobacteria)及软壁菌门(Tenericutes)显著性下调(

0.01)，24个差异菌属中有17个显著性下调(

0.05，

0.01)，7种显著性上调(

0.01)。联合网络可视化分析确定影响程度最大的菌门为拟杆菌门与厚壁菌门，其中厚壁菌门与其余菌门多呈现出负相关的关系，与螺旋体菌门存在最强负相关性；拟杆菌门与变形菌门、厚壁菌门呈正相关的关系，与放线菌门呈现负相关的关系，与其余菌门无相关性相关性；差异菌属多富集于瘤胃球菌属(

Ruminococcus

)，帕拉普氏菌属(

Paraprevotella

)，普雷沃菌属(

Prevotella

)及颤螺旋菌属(

Oscillospira

)。

结论：

黄芩可降低肠道菌群多样性，抑制机体的菌群代谢功能，因此从菌群多样性角度推断黄芩的药性为寒凉。

Abstract

Objective:

To explore the effect of Scutellariae Radix on the diversity of intestinal flora in rats under physiological conditions

in order to determine the property of Scutellariae Radix property.

Method:

16S rRNA high-throughput gene sequencing technique was used to detect the cecum solutes of rats treated with Scutellariae Radix (10 g·kg

-1

). The number

richness and diversity index of the intestinal flora taxon (OTUs) and the differential phylum and genus were comprehensively analyzed. The network visualization was used to find the correlation between differential phylum and genus.

Result:

Based on the Illumina Miseq platform

compared with the blank group

the number of OTUs and the index of richness and diversity of the intestinal flora of the rats treated with Scutellariae Radix decreased. Bacteroidetes and Spirochaetes were significantly up-regulated(

0.01)

while Firmicutes

Actinobacteria

Proteobacteria and Tenericutes were significantly down-regulated(

0.01). Among the 24 differential bacteria genera

17 were significantly down-regulated(

0.05

0.01)

while 7 species were significantly up-regulated(

0.01). On the basis of the network visualization analysis

the most influential phylua were Bacteroides and Firmicutes

in which Firmicutes showed a negative correlation with the rest of phylum

with the strongest negative correlation with spirochetes. There was a positive correlation between Bacteroides

Proteobacteria and Firmicutes

but a negative correlation with Actinobacteria

and no correlation with other phylum. Most of the differential bacteria were enriched in

Ruminococcus

Paraprevotella

Prevotella

and

Oscillospira

Conclusion:

Scutellariae Radix can reduce the diversity of intestinal flora and inhibit the metabolism of the body

so its property is cold.

关键词

Keywords

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