Effect of Dodder Total Flavone on Polycystic Ovary Syndrome Rat Models Induced by Letrozole

Ming-san MIAO; Meng-fan PENG; Xiao-li YAN; Zheng-wang ZHU; Ming BAI; Zhen-zhen WEI; Meng-yan LI

doi:10.13422/j.cnki.syfjx.20191607

您当前的位置：

首页 >

文章列表页 >

Effect of Dodder Total Flavone on Polycystic Ovary Syndrome Rat Models Induced by Letrozole

Pharmacology | 更新时间：2021-02-09

- Effect of Dodder Total Flavone on Polycystic Ovary Syndrome Rat Models Induced by Letrozole
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 16, Pages: 17-23(2019)
- 作者机构：
  
  河南中医药大学，郑州　 450000
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20191607
  CLC： R2-0; R22; R285.5; R289
- Received：02 January 2019，
  
  Published Online：05 May 2019，
  
  Published：20 August 2019
- 稿件说明：
移动端阅览
Ming-san MIAO, Meng-fan PENG, Xiao-li YAN, et al. Effect of Dodder Total Flavone on Polycystic Ovary Syndrome Rat Models Induced by Letrozole[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(16): 17-23.
DOI：

Ming-san MIAO, Meng-fan PENG, Xiao-li YAN, et al. Effect of Dodder Total Flavone on Polycystic Ovary Syndrome Rat Models Induced by Letrozole[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(16): 17-23. DOI： 10.13422/j.cnki.syfjx.20191607.

摘要

目的：

探讨菟丝子总黄酮对来曲唑致大鼠多囊卵巢综合征(PCOS)模型的影响。

方法：

正常组除外，其余大鼠每天灌服来曲唑1 mg·kg

－1

复制PCOS动物模型，连续21 d。造模第16天，阴道涂片检测动物周期，以阴道上皮细胞持续角化者为PCOS成模大鼠，并随机分为模型组、达英-35组(0.339 2 mg·kg

－1

)和菟丝子总黄酮高、中、低剂量组(200，100，50 mg·kg

－1

)。依据组别给予相应药物，连续21 d。给药结束后，取材，计算卵巢指数；酶联免疫吸附测定(ELISA)检测血清雌激素(E

)，睾酮(T)，促性腺激素释放激素(GnRH)，卵泡刺激素(FSH)和促黄体生成素(LH)水平；取大鼠右侧卵巢，苏木素-伊红(HE)染色光镜观察病理改变；免疫组化法检测下丘脑、垂体、左侧卵巢雄激素受体(AR)表达。

结果：

与正常组比较，模型组大鼠血清T，GnRH，LH水平显著升高，卵巢指数和LH/FSH值显著升高，FSH和E

水平显著降低(

0.05，

0.01)，卵巢组织闭锁卵泡增多、卵泡膜细胞层增厚明显，下丘脑、垂体和卵巢组织AR表达显著升高(

0.01)。与模型组比较，菟丝子总黄酮高、中、低剂量组可显著降低PCOS模型大鼠血清T，GnRH和LH水平，降低卵巢指数和LH/FSH值(

0.01)；菟丝子总黄酮高、中剂量组可显著升高PCOS模型大鼠血清E

水平(

0.01)，明显升高FSH水平(

0.05)，菟丝子总黄酮低剂量组可明显升高PCOS模型大鼠血清E

水平(

0.05)。菟丝子总黄酮高、中、低剂量组可不同程度改善PCOS模型大鼠卵巢组织皮质增厚胶原化、闭锁卵泡增多、黄体减少等病理改变。免疫组化结果表明，菟丝子总黄酮高、中、低剂量组可显著降低下丘脑、垂体和卵巢AR表达(

0.01)。

结论：

菟丝子总黄酮可能通过调节雌雄激素分泌、影响下丘脑-垂体-卵巢轴途径发挥对PCOS模型大鼠的保护作用。

Abstract

Objective:

To investigate the effect of dodder total flavone on polycystic ovary syndrome (PCOS) rat models induced by letrozole.

Method:

Except the blank group

the other rats were given letrozole 1 mg·kg

-1

for 21 consecutive days to replicate PCOS animal model. On the 16

day of the modeling

the estrous cycle was detected by vaginal smear

and rat with persistent keratinization of vaginal epithelial cells were selected as the PCOS model rat. The model rats were randomly divided into model group

Dacin-35 group

and high

middle

low-dose dodder total flavonoids groups. The corresponding drugs were given for 21 consecutive days. At the end of the administration

materials were collected to calculate ovary index

and enzyme-linked immunosorbent assay(ELISA) was used to measure estrogen (E

)

testosterone (T)

gonadotropin-releasing hormone (GnRH)

follicle stimulating hormone (FSH) and luteinizing hormone (LH) levels of serum. The right ovary of rats was stained with haematoxylin-eosin (HE)

and the pathological changes were observed by optical microscope. Androgen receptor(AR) expressions in hypothalamus

pituitary and ovary were detected by mmunohistochemistry.

Result:

Compared with the blank group

serum T

GnRH and LH levels

ovarian index and LH/FSH ratio were significantly increased

while FSH and E

levels were significantly decreased (

0.05

0.01). Ovarian tissue follicular atresia increased

and the cell layers of follicular membrane were thickened significantly. AR expressions in hypothalamus

pituitary and ovarian tissues were significantly increased in the model group (

0.01). Compared with the model group

serum E

GnRH and LH levels

ovarian index and LH/FSH ratio were significantly decreased in high

middle and low-dose dodder total flavonoids groups (

0.01); Serum E

level was significantly increased (

0.01)

and FSH level was obviously increased (

0.05) in high and middle-dose dodder total flavonoids group. Serum E

level in PCOS model rats was obviously increased in low-dose dodder total flavonoids group (

0.05). The pathological changes of ovarian tissue of cortical thickening and collagenization

atresia follicle increase and corpus luteum decrease of PCOS model rats were alleviated to different degrees in high

middle and low-dose dodder total flavonoids groups. Immunohistochemical results showed that high

middle and low-dose dodder total flavonoids groups could significantly reduce AR expressions in hypothalamus

pituitary and ovary (

0.01).

Conclusion:

Dodder total flavonoids may play a protective role in PCOS model rats by regulating the secretion of estrogen and androgen and affecting the hypothalamic-pituitary-ovary axis pathway.

关键词

Keywords

references

Yau T T , Ng N Y , Cheung L P , et al . Polycystic ovary syndrome: a common reproductive syndrome with longterm metabolic consequences [J]. Hong Kong Med J , 2017 , 23 ( 6 ): 622 - 634 .

Nasri K , Hantoushzadeh S , Aghadavod E , et al . The effects of omega-3fatty acids supplementation on gene expression involved in the insulin and lipid signaling pathway in patients with polycystic ovary syndrome [J]. Horm Metab Res , 2017 , 49 ( 6 ): 446 - 451 .

彭孟凡，刘保松，苗明三．补肾法治疗多囊卵巢综合征的理论探讨 [J]．中国实验方剂学杂志， 2019 ， 25 ( 6 )： 204 - 209 ．

邵建柱，郭杏林，张婧，等．菟丝子临床应用及其用量 [J]．吉林中医药， 2019 ， 39 ( 1 )： 36 - 39 ．

闫晓丽，张志强，苗明三．基于中西医临床特征的多囊卵巢综合征模型分析 [J]．中医学报， 2017 ， 32 ( 4 )： 598 - 601 ．

苗明三，彭孟凡，闫晓丽．菟丝子总黄酮对多囊卵巢综合征大鼠模型的影响 [J]．中国实验方剂学杂志， 2019 ， 25 ( 5 )： 143 - 150 ．

王施元，李肖肖，于彩虹，等．生长激素调控PCOS大鼠血清与卵巢组织中血管因子表达的研究 [J]．生殖医学杂志， 2018 ， 27 ( 5 )： 444 - 450 ．

Andrade V H , Mata A M , Borges R S , et al . Current aspects of polycystic ovary syndrome: a literature review [J]. Rev Assoc Med Bras , 2016 , 62 ( 9 ): 867 .

周娴颖，周莉，孙祖越．用于治疗多囊卵巢综合征的中药药理学作用机制研究进展 [J]．中国中药杂志， 2016 ， 41 ( 20 )： 3715 - 3720 ．

唐爱军．雌激素联合常规疗法治疗多囊卵巢综合征的临床效果 [J]．河南医学研究， 2018 ， 27 ( 22 )： 4154 - 4155 ．

Mehta R , Kaur M , Gandhi P , et al . Auditory and visual reaction time in polycystic ovarian syndrome [J]. J Clin Diagn Res , 2018 , 12 ( 6 ): CC11 - CC13 .

王靖雅，李文兰，孙向明，等．菟丝子拟雌激素部位在大鼠血清和粪便中的代谢成分分析 [J]．中国药学杂志， 2018 ， 53 ( 11 )： 854 - 861 ．

夏卉芳，王毓．菟丝子水提液对环磷酰胺致雄性小鼠生殖损伤的保护作用研究 [J]．黑龙江畜牧兽医， 2017 ( 21 )： 200-203，297 ．

刘芝，李振彬，刘乐．雷公藤多苷对大鼠卵巢功能耗损的影响与菟丝子总提取物干预作用的实验研究 [J]．湖南中医杂志， 2017 ， 33 ( 4 )： 153 - 155 ．

彭孟凡，乔靖怡，白莉，等．基于关联规则和因子分析探讨中药治疗多囊卵巢综合征用药规律 [J]．时珍国医国药， 2019 ， 30 ( 1 )： 253 - 255 ．

陈蕾，赵陆华，谭喜莹． HPLC法同时测定菟丝子药材中酚酸和黄酮类成分含量 [J]．药学与临床研究， 2018 ， 26 ( 2 )： 101 - 103 ．

赵素霞，刘会丽，江红．菟丝子黄酮通过调节Wnt/β-catenin信号通路干预去卵巢大鼠骨代谢的机制研究 [J]．临床和实验医学杂志， 2018 ， 17 ( 1 )： 25 - 28 ．

任献青，郑贵珍，苏杭，等．菟丝子黄酮对雷公藤多苷片致生精细胞周期阻滞、凋亡及相关蛋白表达降低的影响 [J]．药物评价研究， 2018 ， 41 ( 1 )： 55 - 60 ．

张翌蕾，潘文，汪永娟．多囊卵巢综合征病因病机中西医研究进展 [J]．中国中医基础医学杂志， 2016 ， 22 ( 7 )： 1004 - 1006 ．

WANG Q , Kim J Y , XUE K , et al . Chemerin, a novel regulator of follicular steroidogenesis and its potential involvement in polycystic ovarian syndrome [J]. Endocrinology , 2012 , 153 ( 11 ): 5600 - 5611 .

Shin J J , Park K E , Choi Y M , et al . Early gonadotropin-releasing hormone antagonist protocol in women with polycystic ovarysyndrome: a preliminary randomized tria [J]. Clin Exp Reprod Med , 2018 , 45 ( 3 ): 135 - 142 .

Luque-Ramírez M , Escobar-Morreale H F . Targets to treat androgen excess in polycystic ovary syndrome [J]. Expert Opin Ther Targets , 2015 , 19 ( 11 ): 1545 - 1560 .

Vitek W , Hoeger K , Legro R S . Treatment strategies for infertile women with polycystic ovary syndrome [J]. Minerva Ginecol , 2016 , 68 ( 4 ): 450 - 457 .

张旭帆，郝玲，黄睿臻，等．多囊卵巢综合征患者抗苗勒管激素与LH/FSH相关性分析 [J]．临床检验杂志， 2018 ， 7 ( 4 )： 652 - 653 ．

尹利军，王晓慧．雄激素及其受体在肥胖、肥胖相关疾病及糖脂代谢紊乱中的作用 [J]．生理学报， 2018 ， 70 ( 3 )： 319 - 328 ．

Walters K A . Role of androgens in normal and pathological ovarian function [J]. Reproduction , 2015 , 149 ( 4 ): R193 - R218 .

郭晓东．菟丝子黄酮对大鼠激素型骨质疏松症防护作用及机制研究 [J]．现代中西医结合杂志， 2018 ， 27 ( 23 )： 2525 - 2528 ．

孙晶晶，吴秀娟，鲍军，等．菟丝子总黄酮对氢化可的松致大鼠少弱精子症的治疗作用及其机制 [J]．华西药学杂志， 2016 ， 31 ( 1 )： 14 - 17 ．

佟雅婧，张会仙，陈燕霞，等．马堃教授治疗排卵障碍性不孕用药特点 [J]．中国中药杂志， 2017 ， 42 ( 23 )： 4459 - 4463 ．

罗克燕，杨丹莉，徐敏．菟丝子总黄酮对排卵障碍大鼠下丘脑-垂体-卵巢轴性激素水平的影响 [J]．中国实验方剂学杂志， 2013 ， 19 ( 13 )： 258 - 260 ．

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Effect of Wenshen Zhuyang Tiaozhou Method on Sex Hormones, Insulin and Ovulation in PCOS Patients with Kidney-Yang Deficiency Syndrome Based on PI3K/Akt Signaling Pathway

Effect of Dodder Total Flavone on Polycystic Ovary Syndrome Rat Models

Related Author

Feng-ying LI

Ting-ting ZHAI

Hua YANG

Qi WANG

Ming-san MIAO

Meng-fan PENG

Xiao-li YAN

Related Institution

First Affiliated Hospital of Guizhou University of Traditional Chinese Medicine

Henan University of Traditional Chinese Medicine

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰