Characteristics Analysis for Volatile Compounds of 18 Heat-Clearing Herbs

Xing-xian ZHANG; Xiao-dong SUN; Ke YANG; Tao YU

doi:10.13422/j.cnki.syfjx.20191616

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Characteristics Analysis for Volatile Compounds of 18 Heat-Clearing Herbs

Resource and Quality Evaluation | 更新时间：2021-02-09

- Characteristics Analysis for Volatile Compounds of 18 Heat-Clearing Herbs
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 16, Pages: 111-119(2019)
- 作者机构：
  
  中国药科大学　药学院，南京　 211198
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20191616
  CLC： R284.2; R289; R22; R2-031
- Received：01 February 2019，
  
  Published Online：08 May 2019，
  
  Published：20 August 2019
- 稿件说明：
移动端阅览
Xing-xian ZHANG, Xiao-dong SUN, Ke YANG, et al. Characteristics Analysis for Volatile Compounds of 18 Heat-Clearing Herbs[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(16): 111-119.
DOI：

Xing-xian ZHANG, Xiao-dong SUN, Ke YANG, et al. Characteristics Analysis for Volatile Compounds of 18 Heat-Clearing Herbs[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(16): 111-119. DOI： 10.13422/j.cnki.syfjx.20191616.

摘要

目的：

采用顶空固相微萃取-气相色谱-质谱法(HS-SPME-GC-MS)探究18种清热中药特征性挥发性成分。

方法：

采用HS-SPME萃取药材粉末样品，结合GC-MS对挥发性成分进行定性和定量分析。GC进样口温度为250℃，程序升温，以高纯氦气为载气，柱流量1.0 mL·min

－1

，不分流进样，溶剂延时3 min。MS条件为电子轰击离子源，电子轰击能量70 eV，离子源温度230℃，四极杆温度150℃，扫描范围

29～370。将质谱数据进行谱库NIST2013检索，初步鉴定各中药材的挥发性成分，采用面积归一法计算各组分相对含量，并通过主成分分析(PCA)方法进行数据分析，探讨清热类中药材的挥发性成分特征。

结果：

在18种清热药中共检测到了328种挥发性化合物，主要包括烷烃类、不饱和烃类、醇类、醛类、酮类、酯类、呋喃等。不同清热中药的挥发性成分数量和相对含量差异较大。主成分分析表明，18种清热中药存在一些特征性挥发成分，如左旋樟脑、香榧醇、氧化石竹烯、蓝桉醇和优葛缕酮等。

结论：

清热类中药中含有较为丰富的挥发性成分，既有形成药材特有香气的主要贡献者，又有发挥药效作用的主要活性物质。

Abstract

Objective:

Headspace-solid phase microextraction-gas chromatography-mass spectrometry (HS-SPME-GC-MS) was used to analyze the characteristic volatile compounds from 18 heat-clearing herbs.

Method:

HS-SPME was used to extract the volatile components from different heat-clearing herbs

and the chemical compositions were analyzed by GC-MS. The GC was performed at the injector temperature of 250℃

the high-purity helium was used as the carrier gas at a constant column flow of 1.0 mL·min

-1

with no split sampling. The solvent delay time was 3 min. Mass spectrum conditions included electron bombardment ion source

electron bombardment energy of 70 eV

ion source temperature of 230℃

quadrupoles temperature of 150℃

and scanning range of

29-370.The volatile components were confirmed according to NIST2013 database

and the relative content percentage of each component was calculated by peak area normalization method. PCA was used to detect clustering and discuss the characteristics of volatile compounds of 18 heat-clearing herbs.

Result:

A total of 328 volatile compounds were detected in 18 heat-clearing herbs

including alkanes

unsaturated hydrocarbon

alcohols

aldehyde

ketones

esters

furan and other unknown compounds. The number and relative content of volatile components in different heat-clearing herbs varied dramatically. Several important characteristic volatile compounds

such as

-camphor

torreyol

caryophyllene oxide

globulol and 2

4-cycloheptadien-1-one

6-trimethyl

were detected in the 18 heat-clearing herbs by principal component analysis.

Conclusion:

Heat-clearing herbs contains rich volatile components

which are the main contributors to the formation of unique aroma of herbs

as well as the main active substances.

关键词

Keywords

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