Effect of Aconiti Lateralis Radix Praeparata Polysaccharide on Expressions of MMP-2, MMP-14 in Gastric Cancer Xenografts in Nude Mice

Zhen-xiang AN; Yuan-li HE; Min WANG; Yu-jiang CHEN

doi:10.13422/j.cnki.syfjx.20191621

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Effect of Aconiti Lateralis Radix Praeparata Polysaccharide on Expressions of MMP-2, MMP-14 in Gastric Cancer Xenografts in Nude Mice

Pharmacology | 更新时间：2021-02-09

- Effect of Aconiti Lateralis Radix Praeparata Polysaccharide on Expressions of MMP-2, MMP-14 in Gastric Cancer Xenografts in Nude Mice
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 23, Pages: 79-85(2019)
- 作者机构：
  
  贵州中医药大学第一附属医院，贵阳　 550001
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20191621
  CLC： R22; R242; R2-031; R285.5
- Received：25 December 2018，
  
  Published Online：07 May 2019，
  
  Published：05 December 2019
- 稿件说明：
移动端阅览
Zhen-xiang AN, Yuan-li HE, Min WANG, et al. Effect of Aconiti Lateralis Radix Praeparata Polysaccharide on Expressions of MMP-2, MMP-14 in Gastric Cancer Xenografts in Nude Mice[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(23): 79-85.
DOI：

Zhen-xiang AN, Yuan-li HE, Min WANG, et al. Effect of Aconiti Lateralis Radix Praeparata Polysaccharide on Expressions of MMP-2, MMP-14 in Gastric Cancer Xenografts in Nude Mice[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(23): 79-85. DOI： 10.13422/j.cnki.syfjx.20191621.

摘要

目的：

观察附子多糖对裸鼠胃癌移植瘤的生长抑制作用及对肿瘤组织基质金属蛋白酶-2(MMP-2)，基质金属蛋白酶-14(MMP-14)表达的影响。

方法：

建立胃癌裸鼠移植瘤模型，随机分为模型组，5-氟尿嘧啶组(5-FU，0.01 g·kg

－1

)，附子多糖高、低剂量组(0.2，0.1 g·kg

－1

)，灌胃15 d。观察附子多糖对裸鼠胃癌瘤重的影响；光镜下观察肿瘤细胞的形态变化；酶联免疫吸附法(ELISA)测定血清中转化生长因子-

(TGF-

)含量；通过免疫组化、蛋白质免疫印迹法(Western blot)及实时荧光定量PCR(Real-time PCR)检测肿瘤组织中MMP-2，MMP-14蛋白及mRNA表达水平。

结果：

附子多糖高、低剂量组抑瘤率分别为52.83%，40.57%，与模型组比较，附子多糖高、低剂量组的瘤重显著降低(

0.01)，附子多糖对裸鼠胃癌移植瘤的生长具有抑制作用；ELISA表明，与模型组比较，附子多糖高、低剂量组显著降低裸鼠胃癌血清TGF-

含量(

0.01)；免疫组化表明，与模型组比较，附子多糖高、低剂量组MMP-2蛋白明显下调(

0.05，

0.01)，MMP-14蛋白表达下降(

0.01)；Western blot表明，与模型组比较，附子多糖高、低剂量组MMP-2，MMP-14蛋白表达下调(

0.01)；Real-time PCR结果显示，与模型组比较，附子多糖高剂量组的MMP-2，MMP-14 mRNA表达下降(

0.05，

0.01)。

结论：

附子多糖可抑制裸鼠胃癌移植瘤的生长，抑制TGF-

表达，其抑瘤机制可能与下调MMP-2，MMP-14蛋白及mRNA的表达有关。

Abstract

Objective:

To observe the growth inhibition effect of Aconiti Lateralis Radix Praeparata polysaccharide on the gastric cancer xenografts in nude mice and the expressions of matrix metalloproteinase-2 (MMP-2) and matrix metalloproteinase-14 (MMP-14) in tumor tissues.

Method:

The nude mice xenograft model was established and randomly divided into model group

5-fluorouracil group (5-FU

0.01 g·kg

-1

)

high and low-dose Aconiti Lateralis Radix Praeparata polysaccharide groups (0.2

0.1 g·kg

-1

). Each group was given drug by gavage for 15 days. The effect of Aconiti Lateralis Radix Praeparata polysaccharide on the weight of gastric cancer in nude mice was observed. Morphological changes of tumor cells were observed under light microscope. The content of transforming growth factor-

(TGF-

) in serum was determined by enzyme-linked immunosorbent assay (ELISA). The protein and mRNA expressions of MMP-2

MMP-14 in tumor tissues were detected by immunohistochemistry

Western blot and Real-time PCR.

Result:

The tumor inhibition rates of high and low-dose Aconiti Lateralis Radix Praeparata polysaccharides were 52.83%

40.57%

respectively. Compared with model group

the tumor weight of high and low-dose Aconiti Lateralis Radix Praeparata polysaccharide was significantly lower (

0.01)

indicating the inhibitory effect on the growth of transplanted tumors. ELISA showed that compared with model group

Aconiti Lateralis Radix Praeparata polysaccharide could significantly reduce serum TGF-

content in nude mice (

0.01). Immunohistochemistry showed that compared with model group

MMP-2 protein was down-regulated in high and low-dose Aconiti Lateralis Radix Praeparata polysaccharide groups (

0.05

0.01)

and MMP-14 protein expression was decreased (

0.01). Western blot analysis showed that expressions of MMP-2 and MMP-14 were down-regulated in high and low-dose Aconiti Lateralis Radix Praeparata polysaccharide groups compared with model group (

0.01). Real-time PCR results showed that compared with model group

expressions of MMP-2 and MMP-14 mRNA in high-dose Aconiti Lateralis Radix Praeparata polysaccharide group decreased (

0.05

0.01).

Conclusion:

Aconiti Lateralis Radix Praeparata polysaccharide can inhibit the growth of gastric cancer xenografts in nude mice and the expression of TGF-

.The anti-tumor mechanism may be related to the down-regulation of MMP-2

MMP-14 protein and mRNA expressions.

关键词

Keywords

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