Effect of Sulfur Fumigation on Content of Chemical Constituents in Panacis Quinquefolii Radix and Preliminary Study on Its Identification Method

Shu YAN; Shi-chang XIAO; Wei-qi WANG; Yang YU; Xu-jie SUN; Hui-ying WANG; Lei HOU; Jun JIANG

doi:10.13422/j.cnki.syfjx.20191649

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Effect of Sulfur Fumigation on Content of Chemical Constituents in Panacis Quinquefolii Radix and Preliminary Study on Its Identification Method

Pharmacy and Processing | 更新时间：2021-02-09

- Effect of Sulfur Fumigation on Content of Chemical Constituents in Panacis Quinquefolii Radix and Preliminary Study on Its Identification Method
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 23, Pages: 116-121(2019)
- 作者机构：
  
  1.镇江市食品药品监督检验中心，药品不良反应监测中心，江苏　镇江　 212000；
  2.江苏大学　药学院，医学院，江苏　镇江　 212013；
  3.镇江海关　综合技术中心，江苏　镇江　 212008
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20191649
  CLC： R22; R28; R9; C37; O657; O61
- Received：13 December 2018，
  
  Published Online：08 May 2019，
  
  Published：05 December 2019
- 稿件说明：
移动端阅览
Shu YAN, Shi-chang XIAO, Wei-qi WANG, et al. Effect of Sulfur Fumigation on Content of Chemical Constituents in Panacis Quinquefolii Radix and Preliminary Study on Its Identification Method[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(23): 116-121.
DOI：

Shu YAN, Shi-chang XIAO, Wei-qi WANG, et al. Effect of Sulfur Fumigation on Content of Chemical Constituents in Panacis Quinquefolii Radix and Preliminary Study on Its Identification Method[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(23): 116-121. DOI： 10.13422/j.cnki.syfjx.20191649.

摘要

目的：

阐明硫磺熏蒸对西洋参化学成分含量及品质的影响，探讨硫熏西洋参的鉴别方法。

方法：

采用HPLC-DAD测定西洋参中人参皂苷Rb

，Re，Rg

在硫熏前后的含量变化；采用微波消解法和电感耦合等离子体发射光谱串联质谱(ICP-MS)测定西洋参中11种无机元素在硫熏前后的含量变化；结合主成分分析方法对西洋参硫熏前后的品质差别进行定性区分。

结果：

3种人参皂苷类成分分离良好，在各自的质量浓度范围内与峰面积呈良好的线性，

均≥0.999。硫磺熏蒸前，西洋参中人参皂苷Rg

，Re，Rb

的质量分数分别为(0.897±0.045)，(13.092±0.639)，(12.896±0.430) g·kg

－1

；硫磺熏蒸后，三者的质量分数分别为(0.807±0.032)，(11.968±0.627)，(11.752±0.696) g·kg

－1

；硫熏后，无机元素Cr，Mn，Co，Cu，As，Se，Ag，Cd，Sb，Pb和Fe的含量均有不同程度的增加；综合西洋参中3种有效成分和11种无机元素的含量进行主成分分析，根据三维散点分布结果能够对西洋参硫熏品和非硫熏品进行有效区分。

结论：

硫磺熏蒸后，西洋参中人参皂苷Re和Rb

的含量显著降低，但11种无机元素的含量均有所增加，提示硫磺熏蒸可能会对西洋参品质产生较大影响。

Abstract

Objective:

To elucidate the effect of sulfur fumigation on the content of chemical composition and quality of Panacis Quinquefolii Radix

and to discuss the identification method of sulfur fumigated Panacis Quinquefolii Radix.

Method:

The content changes of ginsenoside Rb

in Panacis Quinquefolii Radix were determined by HPLC-DAD before and after sulfur fumigation. The content changes of 11 inorganic elements in Panacis Quinquefolii Radix before and after sulfur fumigation were detected by microwave digestion method and inductively coupled plasma emission spectrometry tandem mass spectrometry (ICP-MS). The quality difference of Panacis Quinquefolii Radix before and after sulfur fumigation was qualitatively distinguished combined with the principal component analysis.

Result:

Three ginsenosides were well separated and showed good linearity with

≥0.999.Before sulfur fumigation

the contents of ginsenoside Rg

Re and Rb

in Panacis Quinquefolii Radix were (0.897±0.045)

(13.092±0.639)

(12.896±0.430) g·kg

-1

respectively. After sulfur fumigation

the contents of these three components in Panacis Quinquefolii Radix were (0.807±0.032)

(11.968±0.627)

(11.752±0.696) g·kg

-1

respectively. After fumigation

the contents of inorganic elements (Cr

Pb and Fe) from Panacis Quinquefolii Radix increased in varying degrees. According to the distribution results of three-dimensional (3D) scattered points

sulfur and non-sulfur fumigation products of Panacis Quinquefolii Radix could be effectively distinguished.

Conclusion:

After sulfur fumigation

the contents of ginsenoside Re and Rb

in Panacis Quinquefolii Radix decrease significantly

but the contents of 11 inorganic elements increase

suggesting that sulfur fumigation may have a great influence on the quality of Panacis Quinquefolii Radix.

关键词

Keywords

references

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