Preparation and Evaluation of <italic style="font-style: italic">β</italic>-Cyclodextrin Inclusion Complex of Volatile Oil in Couplet Medicines of Moslae Herba and Pogostemonis Herba

Yi-ming KONG; Hui-jie YAN; Feng-qian GUO; Xin-jian LI; Yan TONG; Jin-yu WANG

doi:10.13422/j.cnki.syfjx.20191656

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Preparation and Evaluation of β-Cyclodextrin Inclusion Complex of Volatile Oil in Couplet Medicines of Moslae Herba and Pogostemonis Herba

Pharmacy Fundamentals | 更新时间：2021-02-09

- Preparation and Evaluation of β-Cyclodextrin Inclusion Complex of Volatile Oil in Couplet Medicines of Moslae Herba and Pogostemonis Herba
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 26, Issue 5, Pages: 87-94(2020)
- 作者机构：
  
  中国中医科学院　中药研究所，北京　 100700
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20191656
  CLC： R22; R28; R94; O657.7
- Received：11 April 2019，
  
  Published Online：08 May 2019，
  
  Published：05 March 2020
- 稿件说明：
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Yi-ming KONG, Hui-jie YAN, Feng-qian GUO, et al. Preparation and Evaluation of β-Cyclodextrin Inclusion Complex of Volatile Oil in Couplet Medicines of Moslae Herba and Pogostemonis Herba[J]. Chinese journal of experimental traditional medical formulae, 2020, 26(5): 87-94.
DOI：

Yi-ming KONG, Hui-jie YAN, Feng-qian GUO, et al. Preparation and Evaluation of β-Cyclodextrin Inclusion Complex of Volatile Oil in Couplet Medicines of Moslae Herba and Pogostemonis Herba[J]. Chinese journal of experimental traditional medical formulae, 2020, 26(5): 87-94. DOI： 10.13422/j.cnki.syfjx.20191656.

摘要

目的：

对香薷-广藿香挥发油进行药效学实验考察；建立气相色谱法(GC)同时测定香薷-广藿香挥发油中指标成分麝香草酚、香荆芥酚、百秋李醇含量的方法；优选香薷-广藿香挥发油

-环糊精包合物的制备工艺并确证包合物的形成。

方法：

采用透明质酸酶活性抑制试验建立了体外炎症反应模型，对挥发油进行抗炎测试；采用1，1-二苯基-2-三硝基苯肼(DPPH)自由基清除法对挥发油进行抗氧化活性研究；分别采用饱和水溶液法、胶体磨法、研磨法对香薷-广藿香挥发油进行

-环糊精的包合，采用GC对挥发油中麝香草酚、香荆芥酚、百秋李醇进行含量测定，优选挥发油的提取工艺和包合工艺；通过扫描电子显微镜、红外光谱法、热差分析法及X射线衍射法验证包合物的形成。

结果：

挥发油对透明质酸酶活性有一定程度的抑制作用，对DPPH有消除作用，且随质量浓度的升高而增强。麝香草酚、香荆芥酚、百秋李醇分别在0.021 3～0.426，0.020 04～0.400 8，0.022 6～0.452 g·L

-1

与峰面积呈良好的线性关系(

0.999)；三者的加样回收率分别为99.59%(RSD 1.6%)，100.15%(RSD 1.5%)，100.70%(RSD 1.4%)；胶体磨法测得指标性成分含量最高；扫描电子显微镜、红外光谱法、热差分析法以及X射线衍射法均可验证挥发油

-环糊精包合物的形成。

结论：

挥发油具有一定抗炎活性及抗氧化能力；胶体磨法为香薷-广藿香挥发油最佳包合工艺。建立的GC具有简便灵敏、准确可靠、重复性好等优点，满足同时测定包合物中麝香草酚、香荆芥酚、百秋李醇含量的要求，提高了分析效率，可为该制剂的质量控制研究提供参考。

Abstract

Objective:

To investigate the pharmacodynamics of volatile oil in couplet medicines of Moslae Herba and Pogostemonis Herba

to establish a method for simultaneous determination of three essential ingredients (thymol

carvacrol and patchouli alcohol) in volatile oil of the couplet medicines by gas chromatography (GC)

to optimize the preparation process of

-cyclodextrin (

-CD) inclusion complex of volatile oil in the couplet medicines and to confirm the formation of the inclusion complex.

Method:

in vitro

inflammatory response model was established by hyaluronidase activity inhibition test in order to detect the anti-inflammatory activity of the volatile oil. Also

the antioxidant activity of the volatile oil was assessed by 1

1-diphenyl-2-trinitrophenylhydrazine (DPPH) free radical scavenging method. The inclusion of volatile oil in couplet medicines of Moslae Herba and Pogostemonis Herba was prepared by scaturated aqueous solution method

colloid milling method and grinding method

respectively. GC was used to determine the contents of thymol

carvacrol and patchouli alcohol in volatile oil for optimizing extraction and inclusion processes of volatile oil. Scanning electron microscope

infrared spectroscopy

thermal differential analysis

and X-ray diffraction (XRD) were used to verify the formation of the inclusion complex.

Result:

The volatile oil not only inhibited hyaluronidase activity to a certain extent

but also eliminated DPPH and increased with the increase of concentration. There was a good linear relationship between the peak area and concentration of thymol

carvacrol and patchouli alcohol at 0.021 3-0.426

0.020 04-0.400 8

0.022 6-0.452 g·L

-1

(

0.999)

respectively. Their recoveries were 99.59%(RSD 1.6%)

100.15%(RSD 1.5%)

100.70%(RSD 1.4%)

respectively. The colloid milling method was optimized

and the formation of the inclusion complex was verified by the aforementioned methods.

Conclusion:

The volatile oil in couplet medicines of Moslae Herba and Pogostemonis Herba has certain anti-inflammatory activity and anti-oxidation ability. The colloid milling method was the best inclusion process for the volatile oil. The established GC has the advantages of simple

sensitive

accurate

reliable and reproducible

which can meet the requirements of simultaneous determination of thymol

carvacrol and patchouli alcohol in the inclusion complex.

关键词

Keywords

references

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Preparation and Evaluation of β-Cyclodextrin Inclusion Complex of Volatile Oil in Couplet Medicines of Moslae Herba and Pogostemonis Herba

DOI：10.13422/j.cnki.syfjx.20191656

摘要

Abstract

关键词

Keywords

references