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Effect and Mechanism of Active Fraction of Polyrhachis vicina in Treatment of Systemic Lupus Erythematosus(SLE)
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 18, Pages: 65-70(2019)
- 作者机构：
1.南宁市第三人民医院，南宁 530003；
2.广西壮族自治区中医药研究院，南宁 530022
- 作者简介：
- 基金信息：
DOI：10.13422/j.cnki.syfjx.20191703
CLC： R2-0; R22; R285.5; R289Received：08 February 2019，
Published Online：16 May 2019，
Published：20 September 2019
- 稿件说明：
移动端阅览
Zhang-zhi MAO, Jun-hui HE, Dong-mei LI, et al. Effect and Mechanism of Active Fraction of Polyrhachis vicina in Treatment of Systemic Lupus Erythematosus(SLE)[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(18): 65-70.
Zhang-zhi MAO, Jun-hui HE, Dong-mei LI, et al. Effect and Mechanism of Active Fraction of Polyrhachis vicina in Treatment of Systemic Lupus Erythematosus(SLE)[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(18): 65-70. DOI： 10.13422/j.cnki.syfjx.20191703.
摘要
目的：
2
采用动物模型和细胞模型，探讨拟黑多刺蚁活性组分(AFPV)对系统性红斑狼疮(SLE)的作用及可能机制。
方法：
2
采用大鼠Arthus反应，将60只SD大鼠随机分为正常组，模型组，醋酸泼尼松组(5 mg·kg
－1
)，AFPV高、中、低剂量组(400，200，100 mg·kg
－1
)。采用牛血清蛋白-弗氏完全(不完全)佐剂复制SLE模型。观察AFPV对SLE大鼠背部皮肤红肿直径的影响，酶联免疫吸附测定检测血清抗双链DNA(dsDNA)抗体，补体3(C3)，补体4(C4)，免疫球蛋白M(IgM)，白细胞介素-1(IL-1)，白细胞介素-6(IL-6)，白细胞介素-31(IL-31)，白细胞介素-33(IL-33)水平；免疫磁珠法分选16～18周MRL/lpr狼疮小鼠和C57BL/6J正常小鼠脾脏CD4
＋
T细胞，观察AFPV对miR-200a，miR-155的表达及沉默E盒锌指结合蛋白1(ZEB1)和细胞因子信号转导抑制因子1(SOCS1)水平的影响，探讨AFPV对SLE的作用及可能机制。
结果：
2
与正常组比较，模型组大鼠背部皮肤红肿直径显著增大(
P
<
0.01)，与模型组比较，AFPV高、中剂量组可显著降低SLE大鼠背部皮肤红肿直径(
P
<
0.05，
P
<
0.01)；与正常组比较，模型组大鼠血清IgM，IL-6，IL-33明显增高(
P
<
0.05)，与模型组大鼠比较，AFPV高、中给药组IgM，IL-6，IL-33明显降低(
P
<
0.05)；与正常组比较，MRL/lpr狼疮小鼠的CD4
＋
T细胞miR-200a的表达显著降低，miR-155的表达显著增高及ZEB1水平显著增高而SOCS1的表达显著降低(
P
<
0.01)，与模型组比较，AFPV干预后miR-200a的表达显著增高，miR-155的表达显著降低(
P
<
0.01)，ZEB1水平显著降低而SOCS1的表达显著增高(
P
<
0.01)。
结论：
2
AFPV对SLE大鼠具有一定的治疗作用，其机制可能与其对miR-200a，miR-155及ZEB1 和SOCS1的调节有关。
Abstract
Objective:
2
To observe the effect of an active fraction of
Polyrhachis vicina
(AFPV) on systemic lupus erythematosus (SLE) and its possible mechanism based on animal and cell models.
Method:
2
Totally 60 SD rats were randomly divided into normal control group
model group
prednisone acetate group (5 mg·kg
-1
)
and high
medium and low-dose AFPV groups (400
200
100 mg·kg
-1
). SLE model was replicated with bovine serum albumin-Freund's complete (incomplete) adjuvant. Arthus reaction was observed to study the effect of AFPV on the diameter of back skin redness in rats with SLE. The expressions of anti-double-stranded DNA (dsDNA) antibody
complements 3 (C3)
complement 4 (C4)
immunoglobulin M (IgM)
interleukin-1 (IL-1)
interleukin-6 (IL-6)
interleukin-31 (IL-31) and interleukin-33 (IL-33) in serum were detected by enzyme-linked immunosorbent assay. CD4
+
T cells were isolated from the spleens of MRL/lpr and C57BL/6J mice at the age of 16 to 18 weeks by immunomagnetic beads method. The expressions of miR-200a and miR-155 and the levels of zinc-finger-enhancer binding protein 1(ZEB1) and suppressor of cytokine signaling1(SOCS1) in CD4
+
T cells were observed to explore the effect of AFPV on SLE and its possible mechanism.
Result:
2
Compared with the normal group
the diameter of back skin swelling in the model group was significantly increased (
P
<
0.01). Compared with the model group
the high and medium-dose AFPV groups significantly reduce the skin redness on the back of SLE rats (
P
<
0.05
P
<
0.01); Compared with the normal group
the serum levels of IgM
IL-6 and IL-33 were significantly increased in the model group (
P
<
0.05). Compared with the model group
the serum levels of IgM
IL-6 and IL-33 were significantly decreased after the intervention of AFPV (
P
<
0.05). Compared with the normal group
the expression of miR-200a was significantly decreased (
P
<
0.01)
and its target protein ZEB1 was significantly increased (
P
<
0.01) in the CD4
+
T cells of MRL/lpr lupus mice. Compared with the model group
the expression of microRNA-200a increased significantly
the expression of microRNA-155 decreased significantly (
P
<
0.01)
the level of ZEB1 decreased significantly
and the expression of SOCS1 increased significantly after AFPV intervention(
P
<
0.01).
Conclusion:
2
AFPV has therapeutic effect on rats with SLE
its mechanism may be related to the regulation of miR-200a/ZEB1 and miR-155/SOCS1.
关键词
Keywords
references
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