Methodology for Screening Effective Substances of Chinese Herbal Medicine for Reducing Serum Uric Acid Level

Ling-ling REN; Chi ZHANG; Jin-xiang ZENG; Zhu MAO; Min LI; Ji-xiao ZHU; Yu-ye ZHU; Guo-yue ZHONG; Hong-ning LIU

doi:10.13422/j.cnki.syfjx.20191706

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Methodology for Screening Effective Substances of Chinese Herbal Medicine for Reducing Serum Uric Acid Level

Review | 更新时间：2021-02-09

- Methodology for Screening Effective Substances of Chinese Herbal Medicine for Reducing Serum Uric Acid Level
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 18, Pages: 227-234(2019)
- 作者机构：
  
  江西中医药大学　中药资源与民族药研究中心，南昌　 330004
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20191706
  CLC： R2-0; R22; R285.5; R284.1
- Received：27 February 2019，
  
  Published Online：16 May 2019，
  
  Published：20 September 2019
- 稿件说明：
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Ling-ling REN, Chi ZHANG, Jin-xiang ZENG, et al. Methodology for Screening Effective Substances of Chinese Herbal Medicine for Reducing Serum Uric Acid Level[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(18): 227-234.
DOI：

Ling-ling REN, Chi ZHANG, Jin-xiang ZENG, et al. Methodology for Screening Effective Substances of Chinese Herbal Medicine for Reducing Serum Uric Acid Level[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(18): 227-234. DOI： 10.13422/j.cnki.syfjx.20191706.

摘要

体内血清尿酸浓度长期过高时，可诱发痛风并引起一系列代谢性疾病。近20年来，由于生活水平的不断提高及饮食结构的改变，高尿酸血症及痛风的发病率不断增高。降低血清尿酸水平是高尿酸血症与痛风防治的首要方法，但目前临床应用的降尿酸西药均具有较严重的毒副作用。降尿酸中草药在长期的使用过程中安全有效，随着现代科学技术的迅速发展，应用及开发不同筛选方法从这些中草药中寻找高效、安全的药效物质已成为高尿酸血症及痛风防治药物研究的热点。本文对降尿酸中草药提取物、提取物部位及活性单体成分等药效物质筛选方法进行了综述，发现降尿酸药效物质筛选方法主要有体内法和体外法，体内法主要应用于筛选药材提取物降尿酸活性与机制，体外方法多用于单体活性成分筛选且以黄嘌呤氧化酶(XOD)抑制剂筛选为主，分子对接联用同源建模及亲和超滤联用液质联用(AUF-LC-MS)技术已成为促尿酸排泄成分及XOD抑制剂筛选的新趋势，而体外细胞模型将为促尿酸排泄药中草药药效物质筛选开辟新途径。综述为高尿酸血症及痛风的中草药药药效物质筛选及药物开发提供了一定的参考依据。

Abstract

Gout is caused by the nucleation and growth of monosodium rate crystals in tissues and around joints

which is followed by long-standing hyperuricemia and serum urate of above the saturation threshold. It could cause a series of complications

such as cardiovascular

hypertension

and renal complications. Over the past two decades

the incidences of hyperuricemia and gout have been increasing due to the continuous improvement of living standards and the changes in dietary structure. The prime and most important therapy for hyperuricemia and gout is to reduce serum uric acid levels

but the western medicine for reducing uric acid in clinical application has serious toxic and side effects. With the rapid development of modern science and technology

the application and development of different screening methods for effective ingredients with a low toxicity and side effects from Chinese herbal medicines for reducing serum uric acid levels has attracted much attention in the research and development of drugs for the prevention and treatment of hyperuricemia and gout. In this study

the screening methods for extracts

fractions

active monomer components and other effective substances were reviewed and analyzed. According to the findings

the screening methods had a considerable progress both

in vivo

and

in vitro

. The results showed that the in vivo methods were mainly applied for studying the urate lowing effect and mechanisms of herbal extracts

while the studies for xanthine oxidase(XOD) inhibitors mainly depended on the

in vitro

methods. Molecular docking homology modeling and liquid chromatography-mass spectrometry have become a new trend for screening effective substances with XOD inhibitory activities and uric acid excretion activities

while cell model will open up a new way for screening effective substances for uric acid excretion. The review provides certain reference for effective components screening of hyperuricemia and gout.

关键词

Keywords

references

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