Mechanism of Dichroa Alkali Salt Inducing Vomiting Based on Pica in Mice

Li-na MA; Jian LI; Si-di LI; Bao-qiang DAI; Guang-ping ZHANG; Hong-ping HOU; Ping SU; Feng-yu WANG; Zu-guang YE

doi:10.13422/j.cnki.syfjx.20191722

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Mechanism of Dichroa Alkali Salt Inducing Vomiting Based on Pica in Mice

Pharmacology | 更新时间：2021-02-09

- Mechanism of Dichroa Alkali Salt Inducing Vomiting Based on Pica in Mice
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 22, Pages: 34-41(2019)
- 作者机构：
  
  中国中医科学院　中药研究所，博士后科研流动站，北京　 100700
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20191722
  CLC： R22; R242; R2-031; R285.5
- Received：06 January 2019，
  
  Published Online：22 May 2019，
  
  Published：20 November 2019
- 稿件说明：
移动端阅览
Li-na MA, Jian LI, Si-di LI, et al. Mechanism of Dichroa Alkali Salt Inducing Vomiting Based on Pica in Mice[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(22): 34-41.
DOI：

Li-na MA, Jian LI, Si-di LI, et al. Mechanism of Dichroa Alkali Salt Inducing Vomiting Based on Pica in Mice[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(22): 34-41. DOI： 10.13422/j.cnki.syfjx.20191722.

摘要

目的：

探究常山碱盐(dichroa alkali salt

DAS)诱导呕吐的可能机制。

方法：

采用小鼠异食癖模型，分别观察3种不同机制的镇吐药，多巴胺受体拮抗剂甲氧氯普胺，5-羟色胺3(5-hydroxytryptamine 3，5-HT

)受体拮抗剂昂丹司琼，神经激肽-1受体拮抗剂阿瑞匹坦，对DAS所致小鼠体质量、正常饲料摄食量、高岭土摄食量及腹泻和死亡情况的影响，明确DAS可能的致吐途径；然后采用酶联免疫法针对性的检测DAS干预后不同时间点小鼠回肠和延髓中5-HT和P物质(substance P，SP)的表达情况，以确认DAS是否可影响这2种神经递质的变化。

结果：

配伍昂丹司琼和阿瑞匹坦后，连续给药第4天和药后第1天均可显著改善DAS诱导的小鼠摄食量降低现象(

0.01)，自连续给药第3天起均可显著改善DAS诱导的小鼠体质量降低现象(

0.01)，降低DAS诱导的小鼠腹泻率和死亡率，同时可有效拮抗DAS诱导的小鼠异食行为，但是甲氧氯普胺对上述指标则无明显改善作用；进一步检测呕吐相关神经递质，发现与空白组比较，给予DAS后4，12 h回肠和延髓中5-HT含量显著增加，48 h时回肠和延髓中SP含量显著升高。

结论：

小鼠异食模型可有效表征DAS诱导的呕吐现象，其诱导的呕吐行为可能与回肠和延髓中5-HT和SP含量升高有关，配伍昂丹司琼和阿瑞匹坦可有效拮抗DAS诱导的小鼠异食现象。

Abstract

Objective:

To study the possible mechanism of dichroa alkali salt (DAS) in inducing vomiting.

Method:

Mice pica model was used to observe the antagonistic effect of the three different kinds of antiemetic drugs [dopamine receptor antagonist metoclopramide

5-hydroxytryptamine 3 (5-HT

) receptor antagonist ondansetron and neurokinin-1 receptor antagonist aprepitant]

on body mass

food intake

kaolin consumption

diarrhea and death induced by DAS to preliminarily clarify the possible pathogenic pathway of DAS. Then

the expression of 5-HT and substance P(SP) in ileum and medulla of mice induced by DAS alone at different time points was detected by enzyme-linked immunosorbent assay to confirm whether DAS could affect the changes of these two neurotransmitters.

Result:

After treatment with ondansetron and aprepitant

DAS-induced reduction in food intake of mice was significantly improved on the 4

day after continuous administration and on the 1

day after drug administration (

0.01)

since the 3

day after administration

DAS-induced body mass loss of mice was significantly improved (

0.01)

and DAS-induced diarrhea and mortality in mice were significantly reduced

while DAS-induced pica in mice was effectively antagonized. However

metoclopramide did not significantly improve the above indicators. Further detection of vomiting-related neurotransmitters found that compared with the blank group

the expressions of 5-HT at 4 h

12 h and SP at 48 h after treatment with DAS were significantly increased in ileum and medulla of mice.

Conclusion:

The mice pica model can be used to effectively characterize DAS-induced vomiting. DAS-induced pica in mice may be associated with the increase of 5-HT and SP in ileum and medulla. Ondansetron and aprepitant can effectively antagonize DAS-induced pica in mice.

关键词

Keywords

references

张昌绍，周廷冲．国产抗疟药材之研究(一)常山(临床实验报告) [J]．上海中医药杂志， 1955 ( 8 )： 29 ．

Pandey R K , Kumbhar B V , Srivastava S , et al . Febrifugine analogues as Leishmania donovani trypanothione reductase inhibitors: binding energy analysis assisted by molecular docking, ADMET and molecular dynamics simulation [J]. J Biomol Struct Dyn , 2017 , 35 ( 1 ): 141 - 158 .

江文德，张昌绍，杨藻宸．常山碱催吐作用的研究—I．常山碱对鸽催吐作用的机制 [J]．上海第一医学院学报， 1957 ( 3 )： 253 - 258 ．

江文德．常山碱对狗催吐作用的机制 [J]．生理学报， 1961 ， 24 ( 3/4 )： 180 - 186 ．

Henderson F G , Rose C L , Harris P N , et al . γ-Dichroine, the antimalarial alkaloid of chang shan [J]. J Pharmacol Exp Ther , 1949 , 95 ( 2 ): 191 - 200 .

Rojas C , Slusher B S . Pharmacological mechanisms of 5-HT3 and tachykinin NK1 receptor antagonism to prevent chemotherapy-induced nausea and vomiting [J]. Eur J Pharmacol , 2012 , 684 ( 1/3 ): 1 - 7 .

Saito R , Takano Y , Kamiya H . Roles of substance P and NK1 receptor in the brainstem in the development of emesis [J]. J Pharmacol Sci , 2003 , 91 ( 2 ): 87 - 94 .

GAN T J . Selective serotonin 5-HT3 receptor antagonists for postoperative nausea and vomiting: are they all the same? [J]. Cns Drugs , 2005 , 19 ( 3 ): 225 - 238 .

Fortin S M , Chartoff E H , Roitman M F . The aversive agent lithium chloride suppresses phasic dopamine release through central GLP-1 receptors [J]. Neuropsychopharmacol , 2016 , 41 ( 3 ): 906 - 915 .

CHOU T Q , FU F Y , KAO Y S . Antimalarial constituents of Chinese drug, chang shan, Dichroa febrifuga Lour [J]. J Am Chem Soc , 1948 , 70 ( 5 ): 1765 - 1767 .

Yamamoto K , Asano K , Matsukawa N , et al . Time-course analysis of pica in rats using an automatic feeding monitoring system [J]. J Pharmacol Toxicol , 2010 , 63 ( 1 ): 30 - 34 .

颜艳．灵芝多糖对顺铂诱导的小鼠异食癖和脑内Fos表达的影响 [D]．石家庄：河北师范大学， 2008 ．

李思迪，李春，代宝强，等．常山碱盐急性毒性及其与青蒿素类药物联合用药增效减毒作用 [J]．中国药理学与毒理学杂志， 2016 ， 30 ( 8 )： 808 - 814 ．

李健，杜江，马丽娜，等．常山碱盐灌胃给药抗疟药效及急性毒性 [J]．中国实验方剂学杂志， 2018 ， 24 ( 13 )： 141 - 146 ．

Rudd J A , Yamamoto K , Yamatodani A , et al . Differential action of ondansetron and dexamethasone to modify cisplatin-induced acute and delayed kaolin consumption (“pica”) in rats [J]. Eur J Pharmacol , 2002 , 454 ( 1 ): 47 - 52 .

Mehendale S R , Aung H H , YIN J J , et al . Effects of antioxidant herbs on chemotherapy-induced nausea and vomiting in a rat-pica model [J]. Am J Chinese Med , 2005 , 32 ( 6 ): 897 - 905 .

WANG C Z , Basila D , Aung H H , et al . Effects of Ganoderma lucidum extract on chemotherapy-induced nausea and vomiting in a rat model [J]. Am J Chinese Med , 2005 , 33 ( 5 ): 807 - 815 .

Gershon M D . Review article: serotonin receptors and transporters－roles in normal and abnormal gastrointestinal motility [J]. Aliment Pharm Therap , 2004 , 20 : 3 - 14 .

Mawe G M , Hoffman J M . Serotonin signalling in the gut－functions, dysfunctions and therapeutic targets [J]. Nat Rev Gastroenterol Hepatol , 2013 , 10 ( 8 ): 473 - 486 .

王迎寒，刘玉玲，赵素微，等．腹泻型IBS脾虚肝郁证候与5-HT，CRH的相关性 [J]．中国实验方剂学杂志， 2017 ， 23 ( 18 )： 124 - 129 ．

陈锡强，刘可春，韩利文，等．醋酸直肠刺激对大鼠肠道P物质、5-HT免疫阳性神经纤维的影响 [J]．中国实验方剂学杂志， 2008 ， 14 ( 11 )： 71 - 73 ．

张婧娴，芮俊乾，陈逸凡，等．四逆散有效成分对内脏高敏感大鼠5-HT信号通路的多靶点协同调控 [J]．中国实验方剂学杂志， 2018 ， 24 ( 16 )： 115 - 123 ．

Mantyh P W . Neurobiology of substance P and the NK1 receptor [J]. J Clin Psychiat , 2002 , 63 : 6 - 10 .

Andrews P L R , Bhandari P . Resinferatoxin, an ultrapotent capsaicin analogue, has anti-emetic properties in the ferret [J]. Neuropharmacology , 1993 , 32 ( 8 ): 799 - 806 .

LAI J P , LAI S , Tuluc F , et al . Differences in the length of the carboxyl terminus mediate functional properties of neurokinin-1 receptor [J]. Proc Natl Acad Sci USA , 2008 , 105 ( 34 ): 12605 - 12610 .

Kennedy P G E , Rodgers J , Jennings F W , et al . A substance P antagonist, RP-67, 580, ameliorates a mouse meningoencephalitic response to Trypanosoma brucei [J]. Proc Natl Acad Sci USA , 1997 , 94 ( 8 ): 4167 - 4170 .

严晶，刘丽娜，陆茵，等．金荞麦提取物对肠易激综合征大鼠肥大细胞PAR-2，SP的影响 [J]．中国实验方剂学杂志， 2018 ， 24 ( 2 )： 92 - 97 ．

Bang R , Sass G , Kiemer A K , et al . Neurokinin-1 receptor antagonists CP-96, 345 and L-733, 060 protect mice from cytokine-mediated liver injury [J]. J Pharmacol Exp Ther , 2003 , 305 ( 1 ): 31 - 39 .

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