Simultaneous and Rapid Detection of Concentrated Liquid of Compound Dahuang Decoction Based on Near Infrared Spectroscopy

Hui-min HANG; Ai-ling ZHANG; Wu-jiu WEN; Qing TAO; Xiao-mei YANG; Da-yu CAI; Xiao-jian LUO; Yan HE

doi:10.13422/j.cnki.syfjx.20191747

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Simultaneous and Rapid Detection of Concentrated Liquid of Compound Dahuang Decoction Based on Near Infrared Spectroscopy

Pharmacy and Processing | 更新时间：2021-02-09

- Simultaneous and Rapid Detection of Concentrated Liquid of Compound Dahuang Decoction Based on Near Infrared Spectroscopy
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 26, Issue 4, Pages: 126-132(2020)
- 作者机构：
  
  1.江西中医药大学，南昌　 330004
  2.中药固体制剂制造技术国家工程研究中心，南昌　 330006
  3.深圳市蛇口人民医院，广东深圳　 518067
  4.楚天科技股份有限公司，湖南宁乡　 410600
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20191747
  CLC： R22; O657.33; R28; C37; R94
- Received：10 April 2019，
  
  Published Online：10 May 2019，
  
  Published：20 February 2020
- 稿件说明：
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Hui-min HANG, Ai-ling ZHANG, Wu-jiu WEN, et al. Simultaneous and Rapid Detection of Concentrated Liquid of Compound Dahuang Decoction Based on Near Infrared Spectroscopy[J]. Chinese journal of experimental traditional medical formulae, 2020, 26(4): 126-132.
DOI：

Hui-min HANG, Ai-ling ZHANG, Wu-jiu WEN, et al. Simultaneous and Rapid Detection of Concentrated Liquid of Compound Dahuang Decoction Based on Near Infrared Spectroscopy[J]. Chinese journal of experimental traditional medical formulae, 2020, 26(4): 126-132. DOI： 10.13422/j.cnki.syfjx.20191747.

摘要

目的：

采用近红外光谱技术检测复方大黄汤浓缩过程中浓缩液密度、含固量、大黄酸和甘草酸质量浓度4个指标，建立该复方浓缩过程中多项指标的快速定量分析方法。

方法：

运用近红外光纤透射光谱法对复方大黄汤浓缩液进行快速测定，通过高效液相色谱法(HPLC)测定大黄酸和甘草酸的含量，选择51个样品进行内部交叉验证，采用偏最小二乘法回归分别建立近红外光谱与密度、含固量和指标成分含量之间的校正模型，应用采集的10个未知浓缩液样品进行外部验证预测。

结果：

复方大黄汤浓缩液近红外光谱与密度、含固量、大黄酸及甘草酸质量浓度的外部验证复相关系数(

)分别为0.995 9，0.999 6，0.997 0和0.992 2，预测均方根误差(RMSEP)分别为2.50×10

-3

，0.17，7.57，67.10。

结论：

近红外光谱技术适用于复方大黄汤浓缩液的评价指标测定，且具有分析快速、简便、稳定可靠的特点。

Abstract

Objective:

Near infrared spectroscopy was used to detect the concentration density (25 ℃)

solid-containing content

rhein content and glycyrrhizic acid content of compound Dahuang decoction.

Method:

The concentrated liquid of compound Dahuang decoction was determined by near infrared optical fiber transmission spectrometry. The contents of rhein and glycyrrhizic acid were determined by HPLC. Fifty-one samples were used for internal cross-validation

and partial least square regression was used to establish correction models between near-infrared spectrum and density

solid-containing content

rhein content and glycyrrhizic acid content

respectively. Ten unknown concentrated liquid samples were collected for external validation and prediction.

Result:

The external validation complex correlation coefficients between near-infrared spectra and density

solid-containing content

rhein content and glycyrrhizic acid content of the concentrated liquid of compound Dahuang decoction were 0.995 9

0.999 6

0.997 0 and 0.992 2

and the root mean square error of prediction (RMSEP) values were 2.50×10

-3

0.17

7.57 and 67.10

respectively.

Conclusion:

The near infrared spectroscopy is suitable for the determination of evaluation indexes of the concentrated liquid index of compound Dahuang decoction

and has the characteristics of rapid

simple

stable and reliable.

关键词

Keywords

references

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