Molecular Mechanism of Belamcandae Rhizoma and Ephedrae Herba Couplet Medicines in Treating Asthma Based on Network Pharmacology

Wen-jiang ZHENG; Fu-qi XIE; Hui-ting HUANG; Zi-jing PENG; Yu HONG; Hui-li LIAO; Xiao-hong LIU

doi:10.13422/j.cnki.syfjx.20191803

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Molecular Mechanism of Belamcandae Rhizoma and Ephedrae Herba Couplet Medicines in Treating Asthma Based on Network Pharmacology

Data Mining | 更新时间：2021-02-09

- Molecular Mechanism of Belamcandae Rhizoma and Ephedrae Herba Couplet Medicines in Treating Asthma Based on Network Pharmacology
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 19, Pages: 182-194(2019)
- 作者机构：
  
  1.广州中医药大学　第一临床医学院，广州　 510405；
  2.广州中医药大学　第一附属医院，广州　 510405
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20191803
  CLC： R2-0; R22; R285.5
- Received：02 March 2019，
  
  Published Online：10 June 2019，
  
  Published：05 October 2019
- 稿件说明：
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Wen-jiang ZHENG, Fu-qi XIE, Hui-ting HUANG, et al. Molecular Mechanism of Belamcandae Rhizoma and Ephedrae Herba Couplet Medicines in Treating Asthma Based on Network Pharmacology[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(19): 182-194.
DOI：

Wen-jiang ZHENG, Fu-qi XIE, Hui-ting HUANG, et al. Molecular Mechanism of Belamcandae Rhizoma and Ephedrae Herba Couplet Medicines in Treating Asthma Based on Network Pharmacology[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(19): 182-194. DOI： 10.13422/j.cnki.syfjx.20191803.

摘要

目的：

基于网络药理学分析射干-麻黄药对治疗支气管哮喘的有效活性成分，预测其作用靶点，并探讨其潜在作用机制。

方法：

用中药系统药理学数据库(TCMSP)平台收集射干-麻黄药对的活性成分及预测相应的作用靶点；采用STRING数据库与Cytoscape软件构建“药物-活性成分-靶点”网络和蛋白互作网络，通过ClusterProfiler和ClueGO对核心靶点的生物功能和代谢通路进行富集。最后把候选作用靶点映射在京都基因与基因组百科全书(KEGG)通路图上进行分析。

结果：

通过口服生物利用度(OB)和类药性(DL)两个参数筛选得到38个有效活性成分，包括木犀草素(luteolin)，豆甾醇(stigmasterol)，香木叶素(diosmetin)，柚皮素(naringenin)，槲皮素(quercetin)，鸢尾甲黄素A(iristectorigenin A)，异鼠李素(isorhamnetin)等，与疾病相关的候选作用靶点共214个，该文首要分析核心靶点55个，包括RAC-

丝氨酸/苏氨酸蛋白激酶(Akt1)，肿瘤坏死因子(TNF)，分裂原活化蛋白激酶1(MAPK1)，血管内皮生长因子A(VEGFA)，白细胞介素-10(IL-10)，核转录因子-

B抑制因子

(NFKBIA)等，并富集得到多条相关的基因本体(GO)功能和KEGG通路。

结论：

射干-麻黄药对可能是通过调控Th1和Th2，Th17等细胞分化，Asthma，IL-17，磷脂酰肌醇3-激酶(PI3K)/Akt，MAPK，NF-

B，VEGF等信号通路，干预细胞代谢的多个过程，抑制促炎因子的基因表达，发挥治疗支气管哮喘的作用。

Abstract

Objective:

To analyze the effective active ingredients of Belamcandae Rhizoma and Ephedrae Herba couplet medicines(BREH)in the treatment of bronchial asthma based on network pharmacology

in order to predict their potential targets and explore the mechanism.

Method:

Active ingredients and predict their targets were collected from traditional Chinese medicine system pharmacology(TCMSP) database. Drugs-components-targets network and Proteins interations network were built by STRING database and Cytoscape software. ClusterProfiler and ClueGO was used to enrich the biological function and metabolic pathway of core targets. Finally

candidate targets were mapped onto the pictures of correlative pathways.

Result:

The 38 effectively active ingredients were screened out

including luteolin

stigmasterol

diosmetin

naringenin

quercetin

iristectorigenin A

isorhamnetin. There were 214 candidate targets relating to bronchial asthma

and 55 core ones were selected to be mainly studied

including RAC-alpha serine/threonine-protein kinase (Akt1)

tumor necrosis factor (TNF)

mitogen-activated protein kinase 1 (MAPK1)

vascular endothelial growth factor A (VEGFA)

interleukin-10 (IL-10)

NF-kappa-B inhibitor alpha (NFKBIA)

and a number of relevant gene ontology(GO) functions and Kyoto Encyclopedin of Genes and Genomes(KEGG) pathways were enriched.

Conclusion:

BREH may regulate the Th1

Th2 and Th17 cell differentiations

Asthma

IL-17

phosphatidylinositol-3-kinases(PI3K)/Akt

MAPK

NF-

VEGF signaling pathways

so as to interfere the process of cell metabolism

and inhibit gene expression of proinflammatory factor in the treatment of bronchial asthma.

关键词

Keywords

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