Correlation Between Effective Components Content and Color Values of Indigo Naturalis Basde on Chromatometry

Juan-ru LIU; Xiao-mei LIU; Yu-shi LIU; Hong-mei LIU; Xiao-ya WANG; Cun-yan ZHANG; Li GUO

doi:10.13422/j.cnki.syfjx.20191811

您当前的位置：

首页 >

文章列表页 >

Correlation Between Effective Components Content and Color Values of Indigo Naturalis Basde on Chromatometry

Resource and Quality Evaluation | 更新时间：2021-02-09

- Correlation Between Effective Components Content and Color Values of Indigo Naturalis Basde on Chromatometry
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 23, Pages: 165-170(2019)
- 作者机构：
  
  成都中医药大学　药学院，西南道地药材协同创新中心，中药资源系统研究与开发利用国家重点实验室，成都　 611137
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20191811
  CLC： R284.2; R289; R22; R2-031
- Received：18 December 2018，
  
  Published Online：12 June 2019，
  
  Published：05 December 2019
- 稿件说明：
移动端阅览
Juan-ru LIU, Xiao-mei LIU, Yu-shi LIU, et al. Correlation Between Effective Components Content and Color Values of Indigo Naturalis Basde on Chromatometry[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(23): 165-170.
DOI：

Juan-ru LIU, Xiao-mei LIU, Yu-shi LIU, et al. Correlation Between Effective Components Content and Color Values of Indigo Naturalis Basde on Chromatometry[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(23): 165-170. DOI： 10.13422/j.cnki.syfjx.20191811.

摘要

目的：

研究青黛粉末外观颜色及其提取液颜色与靛蓝、靛玉红含量的相关性。

方法：

采用HPLC法测定27批青黛中靛蓝、靛玉红的含量，采用分光测色仪测定青黛粉末及提取液的色度值

，

。运用SPSS 20.0软件对青黛有效成分含量与其色度值进行相关性及多元线性回归分析。

结果：

感官评定青黛粉末外观颜色结果表明，随着粉末颜色加深，分光测色仪测定粉末和提取液的明亮度(

)降低、红-绿色度(

)升高。相关性分析表明，靛蓝含量与青黛粉末

呈负相关(

0.01)，相关性系数为－0.520；与

呈正相关(

0.01)，相关性系数为0.567。靛玉红含量与青黛粉末、提取液的

呈负相关(

0.01)，相关性系数分别为－0.635，－0.580；与

呈正相关(

0.01)，相关性系数分别为0.706，0.493。

结论：

青黛粉末外观颜色较深者，其靛蓝、靛玉红含量较高；青黛粉末

，

与靛蓝、靛玉红含量的相关性比提取液的相关性大；青黛粉末颜色与靛蓝、靛玉红含量具有显著相关性，为青黛快速鉴别方法提供参考依据；采用分光测色仪测定青黛色度值的方法具有稳定性、科学性和可靠性。

Abstract

Objective:

To study the correlation between the color of Indigo Naturalis (powder and it's extracting solution) and effective components (indigo and indirubin) content.

Method:

The contents of indigo and indirubin in 27 batches of Indigo Naturalis were determined by HPLC

and the chromaticity values

and

of powder and extracting solution were determined by color colorimeter. SPSS 20.0 software was used to analyze the correlation and multiple linear regression between the content of active components and the chromaticity values of Indigo Naturalis.

Result:

By visual observing the appearance of the powder

we find that

as the powder color deepened

the brightness values (

) of the powder and extract were decreased

while the red-green value (

) were increased. The correlation analysis showed that the contents of indigo were negatively correlated with the brightness value (

) of the powder(

0.01)

and the correlation coefficient was-0.520

while they were positively correlated with the red-green value (

)(

0.01)

and the correlation coefficient was 0.567.The contents of indirubin were negatively correlated with the brightness value (

) of powder and extract (

0.01)

and the correlation coefficients were-0.635 and-0.580

respectively

while they were positive correlation with the red-green value (

) (

0.01)

and the correlation coefficient were 0.706 and 0.493

respectively.

Conclusion:

The darker the powder color of Indigo Naturalis

the higher the content of indigo and indirubin. The

and

values of indigo powder were more correlated with indigo and indirubin content than with the extracting solution. The color of powder was significantly correlated with the content of indigo and indirubin

which provided reference for rapid identification of Indigo Naturalis. The method of measuring the color of Indigo Naturalis by color colorimeter is stable

scientific and reliable.

关键词

Keywords

references

LI Z H , ZHU C F , AN B P , et al . Indirubin inhibits cell proliferation, migration, invasion and angiogenesis in tumor-derived endothelial cells [J]. Onco Targets Therapy , 2018 , 11 : 2937 - 2944 .

XIE X J , DI T T , WANG Y , et al . Indirubin ameliorates imiquimod-induced psoriasis-like skin lesions in mice by inhibiting inflammatory responses mediated by IL-17A-producing gamma delta T cells [J]. Mol Immunol , 2018 , 101 : 386 - 395 .

Tokuyasu N , Shomori K , Amano K , et al . Indirubin, a constituent of the Chinese Herbal medicine Qing-Dai, attenuates dextran sulfate sodium-induced murine colitis [J]. Yonago Acta Med , 2018 , 61 ( 2 ): 128 - 136 .

刘泽玉．青黛炮制过程中原理与工艺的优化研究 [D]．成都：成都中医药大学， 2011 ．

何晓凤，王露露，张晶．基于色度学原理的颜色分析方法在药物研究领域的应用概况 [J]．药物分析杂志， 2018 ， 38 ( 9 )： 1471 - 1475 ．

裴建国，刘婧，付小梅，等．不同采收期栀子颜色与其3类有效成分相关性研究 [J]．中药材， 2017 ， 40 ( 10 )： 2280 - 2285 ．

马婷婷，龚慕辛，王智民，等．甘草色泽与有效成分含量的相关性研究 [J]．中国中药杂志， 2017 ， 42 ( 19 )： 3776 - 3785 ．

徐曼菲，吴志生，刘晓娜，等．从辨色论质谈中药质量评价方法 [J]．中国中药杂志， 2016 ， 41 ( 2 )： 177 - 181 ．

姚志昂．青黛的质量考察与掺伪研究 [D]．成都：成都中医药大学， 2011 ．

国家药典委员会．中华人民共和国药典．一部 [M]．北京：中国医药科技社， 2015 ： 199 ．

王艳．青黛的化学成分及其结构特征的研究 [D]．成都：四川大学， 2004 ．

王光宁，何瑞瑜，陈天娇，等．广陈皮的外观颜色客观量化及其陈化年份判别模型的建立 [J]．中国实验方剂学杂志， 2018 ， 24 ( 22 )： 22 - 27 ．

王智磊，伍清芳，刘素娟，等．基于色度分析原理的广陈皮贮藏年限判别 [J]．天然产物研究与开发， 2017 ， 29 ( 8 )： 1355 - 1361 ．

徐红霞，吴沂芸，裴瑾，等．红花黄酮类成分与其色度值相关性研究 [J]．中药材， 2018 ， 41 ( 1 )： 49 - 54 ．

胡静，唐小慧，饶桦静，等．西红花色泽与化学成分含量的相关性分析 [J]．中药材， 2018 ， 41 ( 8 )： 1918 - 1922 ．

杨丽，陈鸿平，李雪莲，等．不同“变色”程度枸杞子外观颜色表征与内在色素类成分变化相关性 [J]．中国实验方剂学杂志， 2015 ， 21 ( 8 )： 47 - 50 ．

王海，严铸云，沈昱翔，等．丹参药材的颜色特征与有效成分的相关性研究 [J]．中药新药与临床药理， 2014 ， 25 ( 3 )： 333 - 338 ．

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Content Difference of Effective Components of Cynomorii Herba Between Different Producing Areas and Its Response to Environmental Factors

New Progress in Mechanism of Salviae Miltiorrhizae Radix et Rhizoma in Treatment of Diabetes and Its Complications

Analysis of Effect of Realgar with Different Compatibility Proportions on Transitional Constituents of Indigo Naturalis in Rat Serum Based on UPLC-Q-TOF-MS/MS

HPLC Simultaneous Determination of Four Effective Components in Different Parts of Seeds of

Study on Quality Standards of Shengxue Xiaoban Tablets

Related Author

Bin GE

Xiao-wen MAO

Xin LYU

Mei QI

Yan GUO

Zhi-rong GU

Rui ZHANG

Xin-hui YAO

Related Institution

College of Pharmacy，Gansu University of Chinese Medicine

Gansu Provincial People's Hospital

Heilongjiang University of Chinese Medicine

Anhui Province Key Laboratory of Chinese Medicinal Formula, School of Pharmacy, Anhui University of Chinese Medicine

School of Pharmacy,Shenyang Pharmaceutical University

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰