Study on Mechanism of Dahuang Huanglian Xiexintang in Treatment of Type 2 Diabetes Mellitus Based on Network Pharmacology

Lan-er SHI; Ke-chao NIE; Wen-jing ZHANG; Mei-si ZHENG; Zhi-qin LIN; Zhang-zhi ZHU

doi:10.13422/j.cnki.syfjx.20191840

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Study on Mechanism of Dahuang Huanglian Xiexintang in Treatment of Type 2 Diabetes Mellitus Based on Network Pharmacology

Data Mining | 更新时间：2021-02-09

- Study on Mechanism of Dahuang Huanglian Xiexintang in Treatment of Type 2 Diabetes Mellitus Based on Network Pharmacology
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 18, Pages: 160-166(2019)
- 作者机构：
  
  1.广州中医药大学　第一临床医学院，广州　 510405；
  2.广州中医药大学　第一附属医院，广州　 510405
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20191840
  CLC： R2-0; R285.5; R289
- Received：04 April 2019，
  
  Published Online：04 June 2019，
  
  Published：20 September 2019
- 稿件说明：
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Lan-er SHI, Ke-chao NIE, Wen-jing ZHANG, et al. Study on Mechanism of Dahuang Huanglian Xiexintang in Treatment of Type 2 Diabetes Mellitus Based on Network Pharmacology[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(18): 160-166.
DOI：

Lan-er SHI, Ke-chao NIE, Wen-jing ZHANG, et al. Study on Mechanism of Dahuang Huanglian Xiexintang in Treatment of Type 2 Diabetes Mellitus Based on Network Pharmacology[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(18): 160-166. DOI： 10.13422/j.cnki.syfjx.20191840.

摘要

目的：

基于网络药理学方法探讨大黄黄连泻心汤治疗2型糖尿病的作用机制。

方法：

借助中药系统药理学技术平台(TCMSP)获取大黄黄连泻心汤主要化学成分、对应靶点及靶标基因，借助人类基因数据库(GeneCards)获取2型糖尿病相关靶标基因，将药物靶标基因与疾病靶标基因映射得到大黄黄连泻心汤作用于2型糖尿病的预测靶点。利用Cytoscape3.7.1软件构建中药化合物-靶点网络及蛋白与蛋白相互作用网络(PPI)，利用DAVID 6.8在线工具进行潜在基因的基因本体(GO)分析和京都基因及基因组百科全书(KEGG)通路富集分析。

结果：

大黄黄连泻心汤作用于2型糖尿病的有效成分有17个，相关靶点94个，关键有效成分17个，关键靶点16个。GO分析结果显示，大黄黄连泻心汤治疗2型糖尿病潜在基因的生物功能主要涉及氧化应激、细胞凋亡、蛋白结合、炎症反应等；KEGG通路富集结果显示，大黄黄连泻心汤治疗2型糖尿病潜在基因的通路主要涉及低氧诱导因子(HIF)，肿瘤坏死因子(TNF)，磷脂酰肌醇-3激酶/蛋白激酶B(PI3K/Akt)，核转录因子-

B(NF-кB)，血管内皮生长因子(VEGF)等信号通路。

结论：

大黄黄连泻心汤治疗2型糖尿病是多成分、多靶点、多通路的复杂过程，主要通过参与氧化应激、细胞凋亡、蛋白结合、炎症反应等发挥治疗2型糖尿病的作用。

Abstract

Objective:

To explore the mechanism of Dahuang Huanglian Xiexintang in the treatment of type 2 diabetes mellitus based on network pharmacology.

Method:

Major chemical constituents

corresponding targets and target genes of Dahuang Huanglian Xiexintang were obtained by Traditional Chinese Medicine Systems Pharmacology(TCMSP)

and target genes of type 2 diabetes mellitus were obtained by GeneCards. The target genes of drug and disease were mapped to predict target genes of Dahuang Huanglian Xiexintang for type 2 diabetes mellitus. Cytoscape3.7.1 software was used to construct the compound-target network and protein-protein interaction network (PPI) of traditional Chinese medicine. Gene ontology (GO) analysis of potential genes and enrichment analysis of gene encyclopedia kyoto encyclopedia of genes and genomes (KEGG) pathway were carried out using DAVID 6.8 online tool.

Result:

There were 17 active ingredients

94 related targets

17 key active ingredients and 16 key targets in Dahuang Huanglian Xiexintang on type 2 diabetes mellitus. GO analysis showed that the biological functions of potential genes of Dahuang Huanglian Xiexintang in the treatment of type 2 diabetes were mainly related to oxidative stress

apoptosis

protein binding

inflammatory reaction

et al. KEGG pathway enrichment results showed that the pathways of potential genes of Dahuang Huanglian Xiexintang in the treatment of type 2 diabetes mainly involved hypoxia inducible factor(HIF)

tumor necrosis factor(TNF)

phosphatidylinositol 3 kinase/protein kinase B(PI3K/Akt)

nuclear transcription factor-кB(NF-кB)

and vascular endothelial growth factor(VEGF) signaling pathways.

Conclusion:

Dahuang Huanglian Xiexintang is a complex process of multi-component

multi-target and multi-pathway in the treatment of type 2 diabetes mellitus. It plays an important role in the treatment of type 2 diabetes mellitus by participating in oxidative stress

apoptosis

protein binding and inflammatory reaction.

关键词

Keywords

references

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