Protective Effect of Linggui Zhugan Tang on Bone Injury in ob/ob Mice Based on Gut Microbiota

Li-ping LIU; Ran LI; Nan JIANG; Lan-chuan ZHANG; Song GU

doi:10.13422/j.cnki.syfjx.20191904

您当前的位置：

首页 >

文章列表页 >

Protective Effect of Linggui Zhugan Tang on Bone Injury in ob/ob Mice Based on Gut Microbiota

Classic Prescriptions | 更新时间：2021-02-09

- Protective Effect of Linggui Zhugan Tang on Bone Injury in ob/ob Mice Based on Gut Microbiota
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 20, Pages: 19-24(2019)
- 作者机构：
  
  辽宁中医药大学，沈阳　 110032
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20191904
  CLC： R2-0; R22; R285.5; R289
- Received：24 February 2019，
  
  Published Online：19 June 2019，
  
  Published：20 October 2019
- 稿件说明：
移动端阅览
Li-ping LIU, Ran LI, Nan JIANG, et al. Protective Effect of Linggui Zhugan Tang on Bone Injury in ob/ob Mice Based on Gut Microbiota[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(20): 19-24.
DOI：

Li-ping LIU, Ran LI, Nan JIANG, et al. Protective Effect of Linggui Zhugan Tang on Bone Injury in ob/ob Mice Based on Gut Microbiota[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(20): 19-24. DOI： 10.13422/j.cnki.syfjx.20191904.

摘要

目的：

观察苓桂术甘汤对瘦素缺陷代谢紊乱模型小鼠肠道菌群的影响，探讨其骨保护作用是否与其调节肠道菌群有关。

方法：

采用ob/ob小鼠，实验分为正常组，模型组，苓桂术甘汤组(7.7 g·kg

－1

)，阿托伐他汀组(0.002 6 g·kg

－1

)，每组8只。连续灌胃4周后，采用苏木素-伊红(HE)染色观察股骨和小肠组织形态，蛋白免疫印迹法(Western blot)检测股骨组织碱性磷酸酶(ALP)蛋白表达，免疫荧光法检测小肠法尼醇X受体(FXR)蛋白表达，酶联免疫吸附测定(ELISA)法测定血清白细胞介素-6(IL-6)含量，16S 核糖体RNA(16S rRNA)测序进行小鼠肠道菌群分析。

结果：

与正常组比较，模型组股骨骨小梁变细，骨髓腔脂肪浸润，股骨ALP蛋白下调；小肠上皮损伤，小肠FXR蛋白下调；血清IL-6含量升高；肠道菌群结构发生变化，在门水平，厚壁菌门(Firmicutes)丰度升高(

0.01)，拟杆菌门(Bacteroidetes)丰度降低(

0.01)，在属水平，毛螺菌属(

Lachnospiraceae

)_UCG-001丰度升高(

0.05)，norank_f__拟杆菌属(

Bacteroidales

)_S24-7_group丰度降低(

0.01)；

Lachnospiraceae

_UCG-001与体质量正相关(

＝0.661)，norank_f__

Bacteroidales

_S24-7_group与体质量负相关(

＝－0.622)。与模型组比较，苓桂术甘汤改善股骨和小肠组织形态，上调股骨ALP和小肠FXR表达，降低血清IL-6含量，可以逆转属水平的菌群改变(

0.05)。

结论：

瘦素缺陷代谢紊乱模型小鼠出现骨损伤、肠道菌群紊乱和肠上皮损伤；苓桂术甘汤对瘦素缺陷代谢紊乱模型小鼠的骨损伤具有保护作用，其作用机制可能与其调节肠道菌群有关。

Abstract

Objective:

To observe the effect of Linggui Zhugan Tang on gut microbiota in ob/ob mice

in order to explore whether its bone protective effect is related to its regulatory effect of gut microbiota.

Method:

The ob/ob mice were divided into control group

model group

Linggui Zhugan Tang group

and Atorvastatin group. After administration for 4 weeks

morphological changes in intestine and bone were observed by htoxylin eosin(HE) staining. The protein expression of alkaline phosphatase(ALP) was detected by Western blot in bone. The expression of farniol X receptor(FXR) was detected by in immunofluorescence staining. And the content of interleukin-6(IL-6) in serum was analyzed by the enzyme-linked immunosorbent assay(ELISA) method. Gut microbiota was analyzed by 16S rRNA.

Result:

Compared with control group

the trabecular bone of the femur became thinner

the bone marrow cavity was infiltrated with fat

and the epithelium of the small intestine was damaged in the model group. The protein expressions of ALP and FXR decreased

while the content of IL-6 in serum was increased in the model group. Compared with model group

the protein expressions of ALP and FXR increased

while the content of IL-6 in serum was decreased in the Linggui Zhugan Tang group. At the genus level

compared with control group

the abundance of

Lachnospiraceae

_UCG-001 increased (

0.05)

while that of norank_f__

Bacteroidales

_S24-7_group decreased in model group (

0.01).

Lachnospiraceae

_UCG-001 was positively correlated with the weight of body (

=0.661)

while norank_f__

Bacteroidales

_S24-7_group was negatively correlated with the weight of body (

=-0.622).

Conclusion:

Linggui Zhugan Tang has a protective effect on bone injury in ob/ob mice

and its mechanism may be related to its regulation of gut microbiota.

关键词

Keywords

references

李玲孺，王济，李英帅，等．从痰湿体质角度研究肥胖亚型 [J]．世界中医药， 2013 ， 8 ( 1 )： 18 - 20 ．

黄江荣，李祥华，杜亚明，等．加味苓桂术甘汤对代谢综合征大鼠糖代谢的影响 [J]．中药药理与临床， 2013 ， 29 ( 3 )： 167 - 169 ．

卢文艺，刘莲，黄蔚，等．加味苓桂术甘汤对代谢综合征大鼠抗氧化作用及脂联素表达的影响 [J]．中国实验方剂学杂志， 2016 ， 22 ( 10 )： 97 - 101 ．

马巧灵，张发，刘朝芹，等． 2型糖尿病肠道菌群研究进展 [J]．中国微生态学杂志， 2018 ， 30 ( 11 )： 1361 - 1364 ．

桑婷婷，郭铖洁，郭丹丹，等．中医药通过调节肠道菌群抑制肥胖和炎症相关疾病的进展研究 [J]．中国中药杂志， 2018 ， 43 ( 16 )： 3235 - 3242 ．

柴水琴，李继斌，王宏英，等．罗格列酮通过IRS-1/Akt信号通路改善ob/ob小鼠的认知功能障碍 [J]．中国药理学通报， 2015 ， 31 ( 6 )： 785 - 789 ．

宁辛．乳杆菌T3L改善肥胖小鼠骨量流失作用及肠道免疫机制研究 [D]．哈尔滨：哈尔滨工业大学， 2018 ．

Quach D , Britton R A . Gut microbiota and bone health . [J]. Adv Exp Med Biol , 2017 , 1033 : 47 - 58 .

McCabe I C , Fedorko A , Myers M G , et al . Novel leptin receptor signaling mutants identify location and sex-dependent modulation of bone density, adiposity, and growth [J]. Cell Biochem , 2019 , 120 ( 3 ): 4398 - 4408 .

Michelin R M , Al-Nakkash L , Broderick T L , et al . Genistein treatment increases bone mass in obese, hyperglycemic mice [J]. Diabetes Metab Syndr Obes , 2016 , 9 : 63 - 70 .

Lindenmaier L B , Philbrick K A , Branscum A J , et al . Hypothalamic leptin gene therapy reduces bone marrow adiposity in ob/obmice fed regular and high-fat diets [J]. Front Endocrinol (Lausanne) , 2016 , doi: 10.3389/fendo.2016.00110 http://doi.org/10.3389/fendo.2016.00110 .

张澜川，李然，刘立萍．补肾方与补肾健脾方对快速老化模型小鼠肠和骨碱性磷酸酶蛋白的影响 [J]．中国老年学杂志， 2018 ， 38 ( 14 )： 3442 - 3445 ．

Rios-Arce N D , Collins F L , Schepper J D , et al . Epithelial barrier function in gut-bone signaling [J]. Adv Exp Med Biol , 2017 , 1033 : 151 - 183 .

Sadagurski M , Norquay L , Farhang J , et al . Human IL6 enhances leptin action in mice [J]. Diabetologia , 2010 , 53 ( 3 ): 525 - 535 .

李婷，朱向东，杨意，等．痛泻要方对溃疡性结肠炎大鼠下丘脑中IL-6，IL-6R表达的影响 [J]．中国实验方剂学杂志， 2017 ， 23 ( 11 )： 103 - 108 ．

Id Boufker H , Lagneaux L , Fayyad-Kazan H , et al . Role of farnesoid X receptor (FXR) in the process of differentiation of bone marrow stromal cells into osteoblasts [J]. Bone , 2011 , 49 ( 6 ): 1219 - 1231 .

ZHENG T , KANG J H , Sim J S , et al . The farnesoid X receptor negatively regulates osteoclastogenesis in bone remodeling and pathological bone loss [J]. Oncotarget , 2017 , 8 ( 44 ): 76558 - 76573 .

刘立萍，李然，张立德，等．苓桂术甘汤对瘦素基因缺陷ob/ob小鼠肝FXR/FGF15/SHP途径的影响 [J]．中国实验方剂学杂志， 2018 ， 24 ( 22 )： 107 - 111 ．

齐艳荣，曹海龙，高建新，等．法尼酯X受体与肠道疾病的关系 [J]．中国临床保健杂志， 2017 ， 20 ( 4 )： 466 - 469 ．

朱杰．基于肠道菌群理论：姜黄石膏制剂对糖尿病小鼠血糖干预的研究 [D]．南京：南京中医药大学， 2018 ．

朱宏斌．宏基因组研究高脂饮食诱导小鼠的肥胖易感性与肠道菌群的关系 [D]．重庆：第三军医大学， 2017 ．

Ley R E , Backhed F , Turnbaugh P , et al . Obesity alters gut microbial ecology [J]. Proc Natl Acad Sci USA , 2005 , 102 ( 31 ): 11070 - 11075 .

刘洋，李可峰，董贵俊，等．肠道菌群在运动改善肥胖性骨关节炎中的作用 [J]．体育科学， 2018 ， 38 ( 4 )： 85-91，97 ．

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Action Mechanism of Resolving Dampness and Phlegm of Pinelliae Rhizoma Praeparatum Based on Interconnection Between Lung and Large Intestine

Effect of Gynostemma pentaphyllum Alcohol Extract on Glucose and Lipid Metabolism Disorders in db/db Mice Based on Transcriptomics and Gut Microbiota

Effect of Zishen Tongguan Formula on "Gut-prostate" Axis of Rats with Chronic Non-bacterial Prostatitis Based on 16S rDNA Sequencing

Effect of Berberine-Baicalin Combination on Fecal Microbiota Transplantation-induced Type 2 Diabetes Mellitus Due to Internal Accumulation of Dampness-heat in Mice from Perspectives of Gut Microbiota and Metabolomics

Fufang Kangjiaolv Capsules Treat Anxiety in Rat Model of Chronic Restraint Stress via Microbiota-gut-brain Axis

Related Author

TAO Xingbao

ZHAO Chentao

ZHU Xiaofu

WU Hao

HE Jun

CAO Weiguo

ZHU Yifei

DING Lei

Related Institution

School of Chinese Medicine，Chongqing University of Chinese Medicine

School of Pharmacy，Nanjing University of Chinese Medicine

School of Pharmacy，Dali University，Dali

Post-Doctoral Research Center，Chongqing TCM Hospital

School of Traditional Chinese Medicine（TCM），Chongqing University of Chinese Medicine

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰