Isolation and Identification of Chemical Constituents from <italic style="font-style: italic">Cyclosorus parasiticus</italic>

Wan-gui TANG; Jia-zhen TAN

doi:10.13422/j.cnki.syfjx.20191911

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Isolation and Identification of Chemical Constituents from Cyclosorus parasiticus

Resource and Quality Evaluation | 更新时间：2021-02-09

- Isolation and Identification of Chemical Constituents from Cyclosorus parasiticus
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 20, Pages: 131-135(2019)
- 作者机构：
  
  恩施土家族苗族自治州中心医院，湖北　恩施　 445000
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20191911
  CLC： R284.2; R282.6; R22; R243
- Received：03 November 2018，
  
  Published Online：17 June 2019，
  
  Published：20 October 2019
- 稿件说明：
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Wan-gui TANG, Jia-zhen TAN. Isolation and Identification of Chemical Constituents from Cyclosorus parasiticus[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(20): 131-135.
DOI：

Wan-gui TANG, Jia-zhen TAN. Isolation and Identification of Chemical Constituents from Cyclosorus parasiticus[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(20): 131-135. DOI： 10.13422/j.cnki.syfjx.20191911.

摘要

目的：

研究华南毛蕨(

Cyclosorus parasiticus

)全草乙酸乙酯萃取物的化学成分。

方法：

取华南毛蕨全草，甲醇渗滤提取后得到总浸膏，然后将总浸膏用适量蒸馏水分散，依次用石油醚、乙酸乙酯、正丁醇萃取，对乙酸乙酯萃取物采用硅胶柱色谱，LH-20型羟丙基葡聚糖凝胶(Sephadex LH-20)柱色谱，ODS柱色谱和半制备高效液相色谱法进行分离纯化，通过红外、质谱、核磁共振技术(

H和

C-NMR)和相关文献数据鉴定结构。

结果：

从华南毛蕨的乙酸乙酯萃取物中共分离纯化了16个化合物，分别鉴定结构为大黄素甲醚(

)，

ent

-kaur-16-ene-2

，15

-diol(

)，

ent

-3

-hydroxy-kaur-16-ene(

)，5，7，8，4′-四甲氧基黄酮(

)，齐墩果酸(

)，5-羟基-7，4′-二甲氧基黄酮(

)，1，7-dihydroxyxanthone(

)，瘤节毛蕨素B(

)，槲皮素(

)，芹菜素(

)，山柰酚(

)，阿福豆苷(

)，芹菜素-7-

-吡喃葡萄糖苷(

)，山柰酚-7-

-吡喃葡萄糖苷(

)，

-胡萝卜苷(

)，

-谷甾醇(

)。

结论：

化合物

1～7

和

9～15

为首次从该植物中分离得到，该研究为阐明华南毛蕨的药效物质基础和质量控制提供了理论依据。

Abstract

Objective:

To study the chemical constituents from the ethyl acetate extract of

Cyclosorus parasiticus

Method:

The whole plant of

parasiticus

were repeatedly percolated with MeOH. The MeOH residue was dispersed in H

O and successively extracted with petroleum ether

ethyl acetate and butyl alcohol. The chemical constituents from the ethyl acetate fraction were isolated by silica gel

Sephadex LH-20 and ODS columns

and semi-prep high performance liquid chromatography

and their chemical structures were determined on the basis of IR

NMR analysis (

H and

C-NMR) and spectroscopic data from literatures.

Result:

Sixteen compounds were isolated from the ethyl acetate fraction

and their chemical structures were identified as physcion (

)

ent

-kaur-16-ene-2

-diol (

)

ent

-3

-hydroxy-kaur-16-ene (

)

4′-tetramethoxyflavone (

)

oleanolic acid (

)

5-hydroxy-7

4′-dimethoxyflavone (

)

7-dihydroxyxanthone (

)

interruptin B (

)

quercetin (

)

apigenin (

)

kaempferol (

)

afzelin (

)

apigenin-7-

-glucopyranosid (

)

kaempferol-7-

-glucopyranoside (

)

-daucosterol (

) and

-sitosterol (

Conclusion:

Compounds

1-7

and

9-15

were isolated from the

parasiticus

for the first time. These results provide a foundation for studying the pharmacodynamics material basis and quality control of

parasiticus

关键词

Keywords

references

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Related Institution

Drug Physics Research Center，Guizhou University of Traditional Chinese Medicine

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⁰

Isolation and Identification of Chemical Constituents from Cyclosorus parasiticus

DOI：10.13422/j.cnki.syfjx.20191911

摘要

Abstract

关键词

Keywords

references