Stoichiometric Analysis in Fingerprint of Aqueous Extract of Citri Reticulatae Pericarpium and Spectrum-effect Relationship with Diabetic Cognitive Dysfunction

Li LI; Yi-chun SUN; Yuan-yuan PAN; Jiao WANG; Qing WU; Wei PAN

doi:10.13422/j.cnki.syfjx.20191919

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Stoichiometric Analysis in Fingerprint of Aqueous Extract of Citri Reticulatae Pericarpium and Spectrum-effect Relationship with Diabetic Cognitive Dysfunction

Resource and Quality Evaluation | 更新时间：2021-02-09

- Stoichiometric Analysis in Fingerprint of Aqueous Extract of Citri Reticulatae Pericarpium and Spectrum-effect Relationship with Diabetic Cognitive Dysfunction
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 26, Issue 1, Pages: 147-154(2020)
- 作者机构：
  
  1.贵州医科大学　医学检验学院，贵阳　 550004
  2.贵州医科大学　基础医学院，贵阳　 550025
  3.国药集团同济堂(贵州)制药有限公司，贵阳　 550018
  4.贵州师范大学　贵州省山地环境信息系统与生态环境保护重点实验室，贵阳　 550001
  5.贵州医科大学　附属医院　贵州省产前诊断中心，贵阳　 550004
  6.贵州医科大学　环境污染与疾病监控教育部重点实验室，贵阳　 550000
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20191919
  CLC： R289; R285; R22; R2-031
- Received：24 March 2019，
  
  Published Online：20 June 2019，
  
  Published：05 January 2020
- 稿件说明：
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Li LI, Yi-chun SUN, Yuan-yuan PAN, et al. Stoichiometric Analysis in Fingerprint of Aqueous Extract of Citri Reticulatae Pericarpium and Spectrum-effect Relationship with Diabetic Cognitive Dysfunction[J]. Chinese journal of experimental traditional medical formulae, 2020, 26(1): 147-154.
DOI：

Li LI, Yi-chun SUN, Yuan-yuan PAN, et al. Stoichiometric Analysis in Fingerprint of Aqueous Extract of Citri Reticulatae Pericarpium and Spectrum-effect Relationship with Diabetic Cognitive Dysfunction[J]. Chinese journal of experimental traditional medical formulae, 2020, 26(1): 147-154. DOI： 10.13422/j.cnki.syfjx.20191919.

摘要

目的：

建立陈皮水提物的HPLC指纹图谱，并研究其与糖尿病大鼠认知功能的谱效关系。

方法：

采用HPLC建立21批陈皮水提物的指纹图谱，流动相乙腈(A)-0.1%的磷酸(B)梯度洗脱，检测波长270 nm；采用指纹图谱软件中的相似度评价，SPSS 24.0二维聚类分析和SIMCA 14.1主成分分析对结果进行分析；采用腹腔注射链脲佐菌素(STZ，50 mg·kg

-1

)复制糖尿病大鼠模型，模型复制成功后给予陈皮水提物灌胃，定期检测空腹血糖值，诱导第12周Morris水迷宫检测实验大鼠的空间学习记忆能力，灰色关联度分析和偏最小二乘回归分析研究谱效关系。

结果：

确定了陈皮水提物中25个共有峰，鉴定出5种物质，并且将21个不同品种、产地的陈皮分成了2类；与模型组比较，灌胃陈皮水提物的各组大鼠空腹血糖下降，认知能力得到改善；灰色关联分析及最小偏二乘回归分析谱效关系表明，19，15，4，17，6号峰与糖尿病大鼠认知功能关联性较大。

结论：

不同品种、产地之间的陈皮水提物存在一定的差异，通过化学计量学可以方便、快捷的对不同产地的陈皮水提取物质进行全面的质量评价，并且陈皮水提物可降低糖尿病大鼠的空腹血糖值及改善认知能力，其发挥作用是多种有效成分共同起效的结果，其中19，15，4，17，6号峰是与糖尿病大鼠认知功能密切相关的成分。

Abstract

Objective:

To establish the HPLC fingerprint of aqueous extract of Citri Reticulatae Pericarpium

and research the spectrum-effect relationship in diabetic cognitive dysfunction rats.

Method:

The HPLC fingerprints of 21 batches of Citri Reticulatae Pericarpium were established

with acetonitrile (A)-0.1%of phosphoric acid (B) as the flow phase for gradient elution. The detection wavelength was 270 nm. Acomparative analysis was performed for the results based on similarity evaluation

SPSS 24.0 two-dimension clustering analysis and SIMCA 14.1 principal component analysis of fingerprint software. Thediabetic rat model wasduplicated by one-time intraperitoneal injection with streptozotocin (STZ) at the dose of 50 mg·kg

-1

. After successful replication of the model

thediabetic rats were given aqueous extract of Citri Reticulatae Pericarpium by gavage. The blood glucose value was determined at regular intervals. The cognitive function was analyzed with Morris water maze experiment at the 12 week. Grey relational analysis (GRA) and partial least squares regression analysis were used to study the spectrum-effect relationship.

Result:

Totally 16 common peaks of water extract of Citri Reticulatae Pericarpium were confirmed

five substances were identified

and 21 species of Citri Reticulatae Pericarpium of different varieties and origins were divided into 2 categories. Compared with the model group

the blood glucose of rats in each group treated with water extract of Citri Reticulatae Pericarpiumwere reduced

and their cognitive function was improved.According to the grey correlation analysis and the least partial square regression analysis

the peaks No. 19

17 and 6 were significantly correlated with the cognitive function of diabetic rats.

Conclusion:

There were some differences in aqueous extract of Citri Reticulatae Pericarpium between different varieties and origins. It is convenient and fast to make a comprehensive quality evaluation of aqueous extract of Citri Reticulatae Pericarpium of different varieties and producing areas by stoichiometry

and aqueous extract of Citri Reticulatae Pericarpium can reduce the blood glucose level and improve the cognitive function of diabetic cognitive dysfunction rats

its mechanisms of action are resultedfrom the joint effects of multiple effective components

and peaks No. 19

17 and 6 are components closely related to the cognitive function of diabetic rats.

关键词

Keywords

references

国家药典委员会．中华人民共和国药典：一部 [M]．北京：中国医药科技出社， 2015 ： 191 .

童红梅 . 陈皮中黄酮类化合物药理作用研究进展 [J]．山西中医学院学报， 2010 ， 11 ( 3 )： 75 - 76 .

T Akachi ， Y Shiina ， Y Ohishi ， et al . Hepatoprotective effects of flavonoids from shekwasha ( Citrus depressa ) against D -galactosamine-induced liver injury in rats [J]. J Nutr Sci Vitaminol (Tokyo) ， 2010 ， 56 ( 1 )： 60 - 67 .

X M CHEN ， A R Tait ， D D Kitts . Flavonoid composition of orange peel and its association with antioxidant and anti-inflammatory activities [J]. Food Chem ， 2017 ， 218 ： 15 - 21 .

L O Erukainure ， A T O Ebuehi ， I M Choudhary ， et al . Orange peel extracts：chemical characterization，antioxidant，antioxidative burst，and phytotoxic activities [J]. J Diet Suppl ， 2016 ， 13 ( 5 )： 585 - 594 .

S C Ho ， C T KUO . Hesperidin，nobiletin，and tangeretin are collectively responsible for the anti-neuroinflammatory capacity of tangerine peel ( Citri reticulatae pericarpium ) [J]. Food Chem Toxicol ， 2014 ， 71 ： 176 - 182 .

G H XU ， J C CHEN ， D H LIU ， et al . Minerals，phenolic compounds，and antioxidant capacity of citrus peel extract by hot water [J]. J Food Sci ， 2008 ， 73 ( 1 )： C11 - 8 .

马森 . 瓯柑橘皮的体内抗氧化作用研究 [J]．武夷学院学报， 2010 ， 29 ( 5 )： 27 - 29 .

马森 . 瓯柑橘皮和陈皮降血糖作用研究 [J]．武夷学院学报， 2010 ， 29 ( 2 )： 18 - 20 .

马森 . 瓯柑橘皮和陈皮类黄酮体外抗氧化作用 [J]．畜牧兽医杂志， 2010 ， 29 ( 3 )： 9 - 10＋13 .

傅曼琴，肖更生，吴继军，等 . 广陈皮促消化功能物质基础的研究 [J]．中国食品学报， 2018 ， 18 ( 1 )： 56 - 64 .

罗琥捷，罗美霞，杨宜婷，等 . 不同产地广陈皮水提物的祛痰、理气功效比较研究 [J]．湖北中医药大学学报， 2018 ， 20 ( 5 )： 48 - 50 .

贺燕林，杨中林 . 陈皮不同提取物及橙皮苷部位的抗炎活性比较研究 [J]．亚太传统医药， 2014 ， 10 ( 13 )： 23 - 25 .

陈琼，付远飞，刘惠婷，等 . 陈皮总黄酮干预血管平滑肌细胞糖胺聚糖代谢的机制研究 [J]．中药新药与临床药理， 2019 ， 30 ( 2 )： 179 - 183 .

A Bhusal ， M H Rahman ， I K Lee ， et al . Role of hippocampal lipocalin-2 in experimental diabetic encephalopathy [J]． Front Endocrinol (Lausanne) ， 2019 ， 10 ： 25 .

A Kuhad ， K Chopra . Curcumin attenuates diabetic encephalopathy in rats：behavioral and biochemical evidences [J]． Eur J Pharmacol ， 2007 ， 576 ( 1/3 )： 34 - 42 .

K Alagiakrishnan ， S Sankaralingam ， M Ghosh ， et al . Antidiabetic drugs and their potential role in treating mild cognitive impairment and Alzheimer' s disease [J]． Discov Med ， 2013 ， 16 ( 90 )： 277 - 286 .

杜宇，付剑亮 . 糖尿病脑病危险因素及发病机制研究进展 [J]．国际神经病学神经外科学杂志， 2016 ， 43 ( 4 )： 358 - 362 .

张梅 . 中西医药物治疗糖尿病脑病的研究进展 [J]．中医药导报， 2014 ， 20 ( 1 )： 109 - 111 .

张红蕊，王耕银，崔昌萌，等 . 川陈皮素对糖尿病大鼠认知功能障碍的影响 [J]．中国老年学杂志， 2018 ， 38 ( 18 )： 4516 - 4519 .

于萍，李克 . 使用Microsoft Excel进行数据的灰关联分析 [J]．微型电脑应用， 2011 ， 27 ( 3 )： 29 - 30 .

白关亚，何盼，李媛媛，等 . 青翘不同极性部位抗炎作用的谱效关系分析 [J]．中国实验方剂学杂志， 2017 ， 23 ( 11 )： 1 - 6 .

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