Suanzaoren Tang Exerts Neuroprotective Effect by Inhibiting Hippocampal Neuroinflammation in APP/PS1 Double Transgenic Mice

Qing-hua LONG; Li DING; Bin-bin ZHAO; Bo XU; Ai-hua TAN; Ping WANG

doi:10.13422/j.cnki.syfjx.20191936

您当前的位置：

首页 >

文章列表页 >

Suanzaoren Tang Exerts Neuroprotective Effect by Inhibiting Hippocampal Neuroinflammation in APP/PS1 Double Transgenic Mice

Classic Prescriptions | 更新时间：2021-02-09

- Suanzaoren Tang Exerts Neuroprotective Effect by Inhibiting Hippocampal Neuroinflammation in APP/PS1 Double Transgenic Mice
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 20, Pages: 1-6(2019)
- 作者机构：
  
  湖北中医药大学　老年医学研究所，武汉　 430065
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20191936
  CLC： R2-0; R285.5; R289
- Received：25 April 2018，
  
  Published Online：19 June 2019，
  
  Published：20 October 2019
- 稿件说明：
移动端阅览
Qing-hua LONG, Li DING, Bin-bin ZHAO, et al. Suanzaoren Tang Exerts Neuroprotective Effect by Inhibiting Hippocampal Neuroinflammation in APP/PS1 Double Transgenic Mice[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(20): 1-6.
DOI：

Qing-hua LONG, Li DING, Bin-bin ZHAO, et al. Suanzaoren Tang Exerts Neuroprotective Effect by Inhibiting Hippocampal Neuroinflammation in APP/PS1 Double Transgenic Mice[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(20): 1-6. DOI： 10.13422/j.cnki.syfjx.20191936.

摘要

目的：

观察酸枣仁汤对APP/PS1小鼠海马神经炎症的影响，并探讨其神经保护的可能机制。

方法：

将小鼠随机分为空白组，模型组，多奈哌齐组(0.92 mg·kg

－1

)，酸枣仁汤低、高(12.96，25.92 g·kg

－1

)剂量组。各组连续给药30 d后，采用尼氏染色观察各组小鼠海马齿状回(DG)区病理形态的变化，采用酶联免疫吸附测定(ELISA)检测各组小鼠血清中肿瘤坏死因子-

(TNF-

)和白细胞介素-1

(IL-1

)表达水平，采用实时荧光定量聚合酶链式反应(Real-time PCR)检测各组小鼠海马中TNF-

，IL-1

的mRNA表达水平，采用免疫组化(IHC)和蛋白免疫印迹法(Western blot)检测各组小鼠海马中胶质纤维酸性蛋白(GFAP)和离子钙结合蛋白(IBA1)蛋白表达水平。

结果：

与空白组比较，模型组小鼠海马DG区颗粒细胞排列不均匀，细胞丢失明显，部分神经元胞内尼氏体消失或固缩，血清中TNF-

，IL-1

含量显著升高(

0.01)，小鼠海马中TNF-

，IL-1

mRNA表达显著升高(

0.01)，海马DG区中GFAP，IBA1蛋白表达显著上调(

0.01)；与模型组比较，多奈哌齐组、酸枣仁汤低、高剂量组小鼠海马DG区颗粒细胞排列稍整齐，神经元丢失减轻，且胞内尼氏体消失或固缩情况好转，小鼠血清中TNF-

，IL-1

含量明显降低(

0.05，

0.01)，小鼠海马中TNF-

，IL-1

mRNA表达明显降低(

0.05，

0.01)，小鼠海马DG区中GFAP，IBA1蛋白表达明显降低(

0.05，

0.01)。

结论：

酸枣仁汤能改善APP/PS1双转基因小鼠神经元丢失，其机制可能与其调节小鼠海马神经炎症有关。

Abstract

Objective:

To observe the effect of Suanzaoren Tang on hippocampal neuroinflammation in APP/PS1 mice and to explore its possible mechanism of neuroprotection.

Method:

The mice were randomly divided into blank group

model group

donepezil group(0.92 mg·kg

-1

)

Suanzaoren Tang low and high-dose groups(12.96

25.92 g·kg

-1

). After 30 days of continuous administration in each group

pathological changes of dentate gyrus (DG) in hippocampus of mice in each group were observed by Nissl staining.The levels of tumor necrosis factor-

(TNF-

) and interleukin-1

(IL-1

) in serum of each group were detected by enzyme-linked immunosorbent assay (ELISA). Real-time quantitative polymerase chain reaction (Real-time PCR) was used to detect the mRNA expression levels of TNF-

and IL-1

in hippocampus of each group. The expression levels of glial fibrillary acidic protein (GFAP) and ionic calcium binding protein 1 (IBA1) in hippocampus of each group were detected by immunohistochemical staining (ICH) and Western blot.

Result:

Compared with blank group

the granule cells in DG region were unevenly arranged in model group

with obvious cell loss

and the nissl bodies in some neurons disappeared or condensed

serum TNF-

and IL-1

content significantly increased (

0.01)

mice hippocampus of TNF-

and IL-1

mRNA expression quantity significantly increased (

0.01)

levels of GFAP and IBA1 in the hippocampal DG region were significantly up-regulated (

0.01). Compared with model group

the granule cells in the hippocampal DG region were slightly aligned in donepezil group

Suanzaoren Tang low and high-dose groups

neuron loss of ease

and intracellular austenite disappear or pyknotic conditions improve

the expressions levels of TNF-

and IL-1

in the serum were decreased(

0.05

0.01)

the expression levels of TNF-

and IL-1

mRNA in the hippocampus were decreased(

0.05

0.01)

protein expression levels of GFAP and IBA1 in the hippocampal DG region were decreased(

0.05

0.01).

Conclusion:

Suanzaoren Tang can improve neuronal loss in APP/PS1 double transgenic mice

and its mechanism may be related to the regulation of hippocampal neuroinflammation in mice.

关键词

Keywords

references

Nirzhor S S R , Khan R I , Neelotpol S . The biology of glial cells and their complex roles in Alzheimer' s disease: new Opportunities in therapy [J]. Biomolecules , 2018 , 8 ( 3 ): 1 - 14 .

郑易林，谢琼，肖立，等．抗阿尔茨海默病药物研发进展 [J]．药学进展， 2019 ， 43 ( 2 )： 100 - 110 ．

Morello M , Landel V , Lacassagne E , et al . Vitamin D improves neurogenesis and cognition in a mouse model of Alzheimer' s disease [J]. Mol Neurobiol , 2018 , 55 ( 8 ): 6463 - 6479 .

蒋宁，张林，徐玫，等．阿尔茨海默病患者来源的诱导性多能干细胞的建立及其在发病机制及新药研究中的初步应用 [J]．中国药理学与毒理学杂志， 2018 ， 32 ( 9 )： 737 - 738 ．

Baazaoui N , Iqbal K . A Novel Therapeutic approach to treat Alzheimer' s disease by neurotrophic support during the period of synaptic compensation [J]. J Alzheimers Dis , 2018 , 62 ( 3 ): 1211 - 1218 .

郝元元，刚宝芝．阿尔茨海默病与睡眠障碍 [J]．中国神经免疫学和神经病学杂志， 2018 ， 25 ( 2 )： 134 - 137 ．

FANG Y , GAO T , ZHNAG B , et al . Recent advances: decoding Alzheimer' s disease with stem cells [J]. Front Aging Neurosci , 2018 , 10 ( 1 ): 3114 - 3119 .

Tzeng T T , CHEN C C , CHEN C C , et al . The cyanthin diterpenoid and sesterterpene constituents of hericium erinaceus mycelium ameliorate Alzheimer' s disease-related pathologies in APP/PS1 transgenic mice [J]. Int J Mol Sci , 2018 , 19 ( 2 ): 1 - 14 .

王新，冯诗瑶，吴昊，等．张仲景治虚烦不眠方之证治概要与方药分析 [J]．中国民族民间医药， 2019 ， 28 ( 3 )： 59 - 62 ．

薄文集．痴呆与失眠相关性的中医理论探讨及酸枣仁汤对APP/PS1双转基因小鼠的干预作用 [D]．武汉：湖北中医药大学， 2018 ．

房蕴歌，商利娜，田雷，等．酸枣仁汤研究进展 [J]．医学研究与教育， 2017 ， 34 ( 3 )： 61 - 67 ．

杨楠，叶晓川．酸枣仁汤的神经药理作用及分子机制研究进展 [J]．湖北中医药大学学报， 2017 ， 19 ( 3 )： 114 - 117 ．

XU X F , WANG Y C , ZONG L , et al . Elevating integrin-linked kinase expression has rescued hippocampal neurogenesis and memory deficits in an AD animal model [J]. Brain Res , 2018 , 95 ( 16 ): 65 - 77 .

姜霞．空间学习对AD模型鼠海马突触可塑性及神经发生的影响 [D]．武汉：华中科技大学， 2012 ．

Minett T , Classey J , Matthews F E , et al . Microglial immunophenotype in dementia with Alzheimer' s pathology [J]. J Neuroinflamm , 2016 , 13 ( 1 ): 135 .

彭也，朱天碧，张钊，等．阿尔茨海默病的免疫调控研究进展 [J]．中国药理学通报， 2019 ， 35 ( 4 )： 445 - 451 ．

尹超，游秋云，张美娅，等．生慧汤对慢性睡眠剥夺小鼠学习记忆及海马IL-6，TNF-α，COX-2基因表达的影响 [J]．中国实验方剂学杂志， 2019 ， 25 ( 1 )： 96 - 100 ．

Bachstetter A D , Van Eldik L J , Schmitt F A , et al . Disease-related microglia heterogeneity in the hippocampus of Alzheimer' s disease, dementia with Lewy bodies, and hippocampal sclerosis of aging [J]. Acta Neuropathol Commun , 2015 , doi: 10.11861S40478-015-0209-z http://doi.org/10.11861S40478-015-0209-z .

刘文．多奈哌齐药理及临床应用及其进展 [J]．天津药学， 2014 ， 26 ( 3 )： 64 - 67 ．

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Mechanism of Huanglian Jiedutang in Improving Pyroptosis， Neuroinflammation， and Learning and Cognitive Functions in APP/PS1 Mice Based on NLRP3/Caspase-1/GSDMD Pathway

Protective Effect and Mechanism of Anmeidan against Neuronal Damage in Rat Model of Sleep Deprivation Based on Hippocampal Neuroinflammation

Mechanism of Congrong Zonggan Capsules in Improving Neuroinflammation and Cognitive Impairment in 5×FAD Mice Based on NF-κB/NLRP3 Signaling Pathway

Effect of Hei Xiaoyaosan on Neuroinflammation and NLRP3/Caspase-1/GSDMD Signaling Pathway in APP/PS1 Mice

Pharmacological Effect of Berberine on Alzheimer's Disease： A Review

Related Author

CHENG Wei

YANG Shuo

HE Zhangxin

CHEN Wei

TAN Aihua

XIE Guangjing

XU Zixuan

ZHANG Junlu

Related Institution

Medical School，Hubei Minzu University

Central Laboratory，Huanggang Hospital of Traditional Chinese Medicine Affiliated to Hubei University of Chinese Medicine

Hubei Key Laboratory for Germplasm Improvement and Utilization of Dabie Shan Dao-di Herbs，Huanggang Normal University

Hubei University of Chinese Medicine

Hubei Shizhen Laboratory

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰