Effect of Bushen Zhuangdu Recipe on Mitochondrial Apoptotic Pathway in Degenerative Intervertebral Disc Cells in Rats

Wen-ming ZHOU; Yi-feng LIN; Zhen ZHANG; Rong-fei BAI

doi:10.13422/j.cnki.syfjx.20191937

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Effect of Bushen Zhuangdu Recipe on Mitochondrial Apoptotic Pathway in Degenerative Intervertebral Disc Cells in Rats

Pharmacology | 更新时间：2021-02-09

- Effect of Bushen Zhuangdu Recipe on Mitochondrial Apoptotic Pathway in Degenerative Intervertebral Disc Cells in Rats
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 19, Pages: 25-31(2019)
- 作者机构：
  
  1.广州中医药大学，广州　 510000；
  2.广州中医药大学　附属骨伤科医院，广州　 510240；
  3.深圳市沙井人民医院，广东　深圳　 518104；
  4.广东省中医院，广州　 510030
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20191937
  CLC： R2-0; R285.5; R289
- Received：13 April 2019，
  
  Published Online：18 June 2019，
  
  Published：05 October 2019
- 稿件说明：
移动端阅览
Wen-ming ZHOU, Yi-feng LIN, Zhen ZHANG, et al. Effect of Bushen Zhuangdu Recipe on Mitochondrial Apoptotic Pathway in Degenerative Intervertebral Disc Cells in Rats[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(19): 25-31.
DOI：

Wen-ming ZHOU, Yi-feng LIN, Zhen ZHANG, et al. Effect of Bushen Zhuangdu Recipe on Mitochondrial Apoptotic Pathway in Degenerative Intervertebral Disc Cells in Rats[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(19): 25-31. DOI： 10.13422/j.cnki.syfjx.20191937.

摘要

目的：

观察补肾壮督方对大鼠退变椎间盘细胞凋亡的影响，探讨其改善椎间盘退变的机制。

方法：

100只SD雄性大鼠，采用纤维环全层针刺造模，随机分为空白组，模型组，补肾壮督方低、中、高剂量组(0.38，0.77，1.53 g·kg

－1

)，连续中药灌胃4周后，苏木素-伊红(HE)染色观察大鼠椎间盘组织病理学变化；原位末端转移酶标记法(TUNEL)检测退变椎间盘中髓核细胞凋亡率，蛋白免疫印迹法(Western blot)检测椎间盘组织中活化半胱氨酸蛋白酶-3(active Caspase-3)，B淋巴细胞瘤-2(Bcl-2)，细胞色素C(cytC)及Bcl-2相关X蛋白(Bax)蛋白水平。

结果：

与空白组比较，模型组大鼠椎间盘组织病理学评分显著增加，髓核细胞凋亡率明显增加(

0.05)，active Caspase-3，cytC和Bax表达量均明显增加(

0.05)，Bcl-2表达量明显下降(

0.05)。与模型组比较，补肾壮督方低、中、高剂量组组织病理学评分明显降低(

0.05)，髓核细胞凋亡率明显减少(

0.05)，active Caspase-3，cytC及Bax表达量均明显下降(

0.05)，Bcl-2表达量明显增加(

0.05)。

结论：

补肾壮督方可能通过减少active Caspase-3，cytC和Bax蛋白表达，增加Bcl-2蛋白表达，抑制线粒体凋亡通路，并存在一定剂量依赖性，从而改善椎间盘退变。

Abstract

Objective:

To explore the mechanism of mitochondrial apoptotic pathway in rat degenerative intervertebral disc cells in improving intervertebral disc degeneration under the action of Bushen Zhuangdu recipe.

Method:

The 100 SD male rats were randomly divided into blank group

model group

low

medium and high dose Bushen Zhuangdu recipe group (0.38

0.77

1.53 g·kg

-1

). Histopathological changes of rat intervertebral discs were observed by hematoxylin-eosin(HE) staining after 4 weeks of continuous administration of Chinese medicine. The apoptotic rate of nucleus pulposus cells in degenerative intervertebral discs was detected by TUNEL(terminal deoxynucleotidyltransferase-mediated dUTP-biotin nick end labeling)

and the levels of active Cysteinyl aspartate-specific proteinase-3(active Caspase-3)

B cell leukemia-2(Bcl-2)

cytochrome C (cytC) and Bcl-2-associated X protein(Bax) protein in intervertebral discs were detected by Western blot.

Result:

Compared with blank group

the histopathological score of intervertebral disc in the model group was significantly increased

the apoptosis rate of nucleus pulposus was significantly increased (

0.05)

the expression levels of active Caspase-3

cytC and Bax were significantly increased (

0.05)

and the expression levels of Bcl-2 were significantly decreased (

0.05). After the intervention of Bushen Zhuangdu recipe.Compared with model group

the histopathological score of Bushen Zhuangdu recipe low

medium and high dose groups were significantly reduced(

0.05)

the expression levels of active Caspase-3

cytC and Bax were significantly decreased (

0.05)

and the expression levels of Bcl-2 were significantly increased (

0.05).

Conclusion:

Bushen Zhuangdu recipe may improve the degeneration of intervertebral disc by reducing the expression of active Caspase-3

cytC and Bax

increasing the expression of Bcl-2 and inhibiting the apoptotic pathway of mitochondria in a dose-dependent manner.

关键词

Keywords

references

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