Network Pharmacology Research of Wuzi Yanzongwan in Treatment of Male Infertility

Jin DING; Yao-sheng ZHANG; Jian-wei SHANG

doi:10.13422/j.cnki.syfjx.20191940

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Network Pharmacology Research of Wuzi Yanzongwan in Treatment of Male Infertility

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- Network Pharmacology Research of Wuzi Yanzongwan in Treatment of Male Infertility
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 20, Pages: 25-35(2019)
- 作者机构：
  
  1.广州中医药大学　附属深圳市宝安中医院(集团)，广东　深圳　 518133；
  2.北京中医药大学　东直门医院，北京　 100700
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20191940
  CLC： R2-0; R285.5; R289
- Received：30 May 2019，
  
  Published Online：18 June 2019，
  
  Published：20 October 2019
- 稿件说明：
移动端阅览
Jin DING, Yao-sheng ZHANG, Jian-wei SHANG. Network Pharmacology Research of Wuzi Yanzongwan in Treatment of Male Infertility[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(20): 25-35.
DOI：

Jin DING, Yao-sheng ZHANG, Jian-wei SHANG. Network Pharmacology Research of Wuzi Yanzongwan in Treatment of Male Infertility[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(20): 25-35. DOI： 10.13422/j.cnki.syfjx.20191940.

摘要

目的：

基于网络药理学探讨五子衍宗丸治疗男性不育症的物质基础及作用机制。

方法：

应用中药系统药理学分析平台(TCMSP) 筛选五子衍宗丸中的活性成分和作用靶点；应用GeneCards，OMIM，PharmGkb数据库筛选男性不育症的疾病靶点；应用R语言软件筛选药物与疾病共同靶点，应用Cytoscape软件构建药物-活性成分-疾病-靶点互作网络；应用STRING平台构建共同靶点蛋白互作网络(PPI)；应用ClueGo插件对共同靶点进行基因本体(GO)分析，并应用R语言软件对共同靶点进行京都基因与基因组百科全书(KEGG)通路富集。

结果：

从五子衍宗丸中共获得72个有效活性成分，得到35个五子衍宗丸治疗男性不育症的可能靶点，这些靶点主要涉及氧化与抗氧化活性等生物学过程，并主要富集在磷脂酰肌醇3-激酶/蛋白激酶B(PI3K/Akt)，缺氧诱导因子-1(HIF-1)等信号通路上。

结论：

通过网络药理学证实了五子衍宗丸多成分、多靶点、多途径的作用特点，预测了五子衍宗丸治疗男性不育症的可能作用机制，为进一步研究其活性成分和作用机制提供理论依据。

Abstract

Objective:

Exploring the material basis and mechanism of Wuzi Yanzongwan in the treatment of male infertility based on network pharmacology.

Method:

Traditional Chinese medicine systems pharmacology database and analysis platform(TCMSP) was used to screen the active ingredients and targets in Wuzi Yanzongwan. GeneCards

OMIM and PharmGkb databases were used to screen the targets of male infertility. R language software was used to screen common targets of drugs and diseases

Pharmaceutical active ingredients-disease target interaction network was constructed by using Cytoscape software. The common target protein interaction network (PPI) was constructed by STRING platform

the gene ontology(GO) analysis of common target was analyzed by ClueGo plug-in

and Kyoto encyclopedia of genes and genomes(KEGG) pathway was enriched by R language software.

Result:

A total of 72 active ingredients were obtained from Wuzi Yanzongwan

and 35 possible targets for the treatment of male infertility were obtained. These targets are mainly involved in biological processes such as oxidation and antioxidant activity

and are mainly concentrated in phosphatidylinositol 3-kinase/protein kinase B(PI3K/Akt) and hypoxia inducible factor-1(HIF-1) signaling pathways.

Conclusion:

The network pharmacology confirmed the multi-component

multi-target and multi-pathway action characteristics of Wuzi Yanzongwan

predicted the possible mechanism of Wuzi Yanzongwan in the treatment of male infertility

and provided theoretical basis for further study of its active ingredients and mechanism.

关键词

Keywords

references

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