Cloning and Bioinformatic Analysis of ARPI Gene from Glycyrrhiza glabra

Shao-kai TIAN; Jia-ming HOU; Zhi-qiang GAO; Lin YANG; Zhi-xin ZHANG; Ying LIU

doi:10.13422/j.cnki.syfjx.20191951

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Cloning and Bioinformatic Analysis of ARPI Gene from Glycyrrhiza glabra

Resource and Quality Evaluation | 更新时间：2021-02-09

- Cloning and Bioinformatic Analysis of ARPI Gene from Glycyrrhiza glabra
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 26, Issue 4, Pages: 185-190(2020)
- 作者机构：
  
  北京中医药大学　生命科学学院，北京　 102488
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20191951
  CLC： R22; R28; R931; Q78; R318
- Received：29 April 2019，
  
  Published Online：19 June 2019，
  
  Published：20 February 2020
- 稿件说明：
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Shao-kai TIAN, Jia-ming HOU, Zhi-qiang GAO, et al. Cloning and Bioinformatic Analysis of ARPI Gene from Glycyrrhiza glabra[J]. Chinese journal of experimental traditional medical formulae, 2020, 26(4): 185-190.
DOI：

Shao-kai TIAN, Jia-ming HOU, Zhi-qiang GAO, et al. Cloning and Bioinformatic Analysis of ARPI Gene from Glycyrrhiza glabra[J]. Chinese journal of experimental traditional medical formulae, 2020, 26(4): 185-190. DOI： 10.13422/j.cnki.syfjx.20191951.

摘要

目的：

克隆光果甘草生长素/吲哚乙酸蛋白(Aux/IAA)(GgARPI)基因并进行生物信息学分析。

方法：

取光果甘草新鲜主根，提取RNA，通过逆转录-聚合酶链式反应(RT-PCR)克隆

GgARPI

基因互补脱氧核糖核酸(cDNA)序列，测序并对其进行生物信息学分析。

结果：

克隆得到长度为686 bp的

GgARPI

基因cDNA序列，包含1个585 bp的完整开放阅读框(ORF)，编码194个氨基酸残基。生物信息学分析表明

GgARPI

编码蛋白为稳定亲水蛋白，相对分子质量21.95 kDa，等电点6.85，不含信号肽，无跨膜区，二级结构以无规卷曲为主，包含1个Aux/IAA蛋白超家族结构域。同源性分析表明

GgARPI

基因与豆科植物的亲缘关系较近，与单子叶植物谷子

Setaria italica

的进化关系最远。

结论：

首次克隆得到

GgARPI

cDNA序列，为进一步研究

GgARPI

基因的功能及其对甘草酸生物合成的分子调控机制奠定了基础。

Abstract

Objective:

To clone the complementary deoxyribonucleic acid (cDNA) of auxin/indole acetic acid protein (Aux/IAA) from

Glycyrrhiza glabra

(GgARPI) and analyze its sequence by bioinformatics.

Method:

RNA was extracted from fresh root of

glabra

the cDNA sequence of

GgARPI

gene was cloned by reverse transcription polymerase chain reaction (RT-PCR)

then sequencing and bioinformatic analysis were performed.

Result:

The

GgARPI

cDNA sequence with the full length of 686 bp was obtained from

glabra

. The full open reading frame (ORF) was 585 bp

encoding 194 amino acid residues. The bioinformatic analysis showed that the protein coded by

GgARPI

was a stable hydrophilic protein

with a relative molecular weight of 21.95 kDa and isoelectric point of 6.85.It contained no signal peptides or transmembrane domain. Its secondary structure mainly consisted of random coil. An Aux/IAA superfamily was included in the conversed domain. Homology analysis indicated that it had a close evolutionary relationship with leguminous plants

and a distant evolutionary relationship with monocotyledon

such as

Setaria italica

Conclusion:

GgARPI

cDNA sequence is successfully cloned from

glabra

for the first time

which will lay a foundation for studying the function of

GgARPI

and explaining the molecular regulatory mechanism of biosynthesis of glycyrrhizic acid in

glabra

关键词

Keywords

references

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