Effect and Mechanism of Pilose Antler Different Components on Bone Tissue of Ovariectomized Osteoporosis Model Rats

Wei GONG; Hong-xin ZHENG; Dong-xiang YANG; Feng LI; Lei-ming QU; Wei LIU

doi:10.13422/j.cnki.syfjx.20192003

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Effect and Mechanism of Pilose Antler Different Components on Bone Tissue of Ovariectomized Osteoporosis Model Rats

Pharmacology | 更新时间：2021-02-09

- Effect and Mechanism of Pilose Antler Different Components on Bone Tissue of Ovariectomized Osteoporosis Model Rats
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 20, Pages: 36-42(2019)
- 作者机构：
  
  1.辽宁中医药大学，沈阳　 110032；
  2.辽宁中医药大学　附属医院，沈阳　 110032
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20192003
  CLC： R2-0; R22; R285.5
- Received：25 March 2019，
  
  Published Online：08 July 2019，
  
  Published：20 October 2019
- 稿件说明：
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Wei GONG, Hong-xin ZHENG, Dong-xiang YANG, et al. Effect and Mechanism of Pilose Antler Different Components on Bone Tissue of Ovariectomized Osteoporosis Model Rats[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(20): 36-42.
DOI：

Wei GONG, Hong-xin ZHENG, Dong-xiang YANG, et al. Effect and Mechanism of Pilose Antler Different Components on Bone Tissue of Ovariectomized Osteoporosis Model Rats[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(20): 36-42. DOI： 10.13422/j.cnki.syfjx.20192003.

摘要

目的：

考察鹿茸不同组分对去卵巢所致骨质疏松症大鼠骨组织的影响及作用机制，明确鹿茸健骨作用的主要物质基础，并探讨其健骨作用的机制。

方法：

雌性SD大鼠56只，随机分为7组，分别为正常组，模型组，仙灵骨葆组(468 mg·kg

－1

)，补佳乐组(80 mg·kg

－1

)，鹿茸多糖组(50 mg·kg

－1

)，鹿茸多肽组(175 mg·kg

－1

)，鹿茸多糖多肽组(50 mg·kg

－1

＋175 mg·kg

－1

)。采用切除大鼠双侧卵巢的方法复制骨质疏松症模型，连续给药12周后，采用骨密度仪检测大鼠骨密度、酶联免疫吸附测定(ELISA)检测各组大鼠骨组织中骨源性碱性磷酸酶(BALP)，骨钙素(OT)，骨形成蛋白-2(BMP-2)，Smad1，Smad5，Runt相关转录因子2(RUNX2)等指标活性；采用实时荧光定量聚合酶链式反应(Real-time PCR)及蛋白免疫印迹法(Western blot)检测骨组织中BMP-2，Smad1，Smad5，Runx2 mRNA及蛋白的表达水平。苏木素-伊红(HE)染色观察大鼠骨组织进行形态学变化。

结果：

与正常组比较，模型组大鼠骨密度明显降低(

0.05)，骨组织中BALP，OT，BMP-2，Smad1，Smad5，Runx2活性明显降低(

0.05)，骨组织中BMP-2，Smad1，Smad5，Runx2 mRNA及蛋白表达明显降低(

0.05)，骨组织结构破坏；与模型组比较，鹿茸多糖组、鹿茸多肽组、鹿茸多糖多肽组可明显提高去卵巢大鼠骨密度(

0.05)，促进骨组织中BALP，OT，BMP-2，Smad1，Smad5，Runx2活性(

0.05)；上调骨组织中BMP-2，Smad1，Smad5，Runx2 mRNA及蛋白表达(

0.05)，预防骨组织破坏。

结论：

鹿茸不同组分对去卵巢所致的大鼠骨质疏松症均有治疗作用，其作用可能是与上调BMP-2/Smad1，Smad5/Runx2信号通路有关。

Abstract

Objective:

To explore the effect and mechanism of pilose antler different components on the bone tissue of ovariectomized osteoporosis model rats and ascertain the material basis of pilose antler.

Method:

fifty-six SD rats were divided randomly into seven groups: normal group

model group

Xianling Gubao group(468 mg·kg

-1

)

Bujiale group(80 mg·kg

-1

)

polysaccharide group(50 mg·kg

-1

)

polypeptides group(175 mg·kg

-1

)

polysaccharide and polypeptide mixture group(50 mg·kg

-1

+ 175 mg·kg

-1

). Osteoporosis mode was established through ovary resection of female rats

meanwhile

the rats were given different components of pilose antler for consecutively 12 weeks. Subsequently

using absorptiometry to measure the rats' bone mass density. The activities of bone alkaline phosphatase(BALP)

osteocalcin (OT)

bone morphogenetic protein2(BMP-2)

Smad1

Smad5

Runt-related transcription factor 2 (RUNX2) were detected by enzyme-linked immuno sorbent assay (ELISA). The expression of BMP-2

Smad1

Smad5

Runx2 protein was examined by Western blot and Real-time polymerase chain reaction (Real-time PCR). Morphological assay for bone tissue were detected by htoxylin eosin(HE) staining.

Result:

After 12 weeks

Compared with the normal group

the osteoporosis model group showed significantly decrease in bone mineral density(

0.05)

and bone BALP

BMP-2

Smad1

Smad5

Runx2 content(

0.05)

the mRNA and protein expression of BMP-2

Smad1

Smad5

Runx2 in bone were significantly decrease(

0.05). Bone tissue was destroyed. Compared with the osteoporosis model group

different components of pilose antler could significantly improve the activities of BALP

BMP-2

Smad1

Smad5

Runx2 and bone mineral density in bone

and up-regulate the mRNA and protein expression of BMP-2

Smad1

Smad5

Runx2.Bone structure is repaired.

Conclusion:

Pilose antler different components has therapeutic effect on ovariectomized osteoporosis model rats.The mechanism may be related to up-regulat the expression of BMP-2/Smad1

Smad5/Runx2 signal pathways.

关键词

Keywords

references

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