Anti-inflammatory Effect of Dendrobii Huoshanense Herba

Meng-meng WANG; Zhao-jie JI; Jiang-hua GAN; Nian-jun YU; Wei-dong CHEN; Dai-yin PENG; Lan HAN

doi:10.13422/j.cnki.syfjx.20192004

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Anti-inflammatory Effect of Dendrobii Huoshanense Herba

Pharmacology | 更新时间：2021-02-09

- Anti-inflammatory Effect of Dendrobii Huoshanense Herba
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 20, Pages: 76-81(2019)
- 作者机构：
  
  安徽中医药大学　药学院，中药复方安徽省重点实验室，安徽道地中药材品质提升协同创新中心，合肥　 230012
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20192004
  CLC： R2-0; R22; R285.5
- Received：12 December 2018，
  
  Published Online：05 July 2019，
  
  Published：20 October 2019
- 稿件说明：
移动端阅览
Meng-meng WANG, Zhao-jie JI, Jiang-hua GAN, et al. Anti-inflammatory Effect of Dendrobii Huoshanense Herba[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(20): 76-81.
DOI：

Meng-meng WANG, Zhao-jie JI, Jiang-hua GAN, et al. Anti-inflammatory Effect of Dendrobii Huoshanense Herba[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(20): 76-81. DOI： 10.13422/j.cnki.syfjx.20192004.

摘要

目的：

观察霍山石斛的抗炎作用。

方法：

60只昆明种小鼠，随机分为正常组、模型组、石斛高、中、低剂量组(4，2，1 g·kg

－1

)，醋酸地塞米松组(醋酸地塞米松，0.01 g·kg

－1

)；连续灌胃给药14 d；末次给药1 h后，于小鼠右耳的耳中部两侧滴二甲苯20 μL建立耳肿胀模型。1 h后断头处死所有小鼠，计算各组小鼠耳肿胀率。健康SD大鼠36只，分别为正常组、模型组、霍山石斛高、中、低(2.8，1.4，0.7 g·kg

－1

)剂量组、醋酸地塞米松组。连续灌胃给药7 d。末次灌胃给药1 h后，于各组大鼠的右后肢足趾皮下注射10%新鲜鸡蛋清建立足肿胀模型。分别于0，0.5，1，2，3，4，5 h时，测量各大鼠的右后足周长，计算大鼠的足肿胀度。取SD大鼠60只，向双侧腹股沟皮下植入无菌干燥棉球，分组同上。第2天灌胃给药，连续14 d。末次给药后，取出棉球，计算肉芽肿抑制率；检测各组大鼠血清中肿瘤坏死因子-

(TNF-

)，白细胞介素-1

(IL-1

)，白细胞介素-2(IL-2)，白细胞介素-6(IL-6)，

-干扰素(IFN-

)含量。

结果：

与正常组比较，模型组耳肿胀率、足肿胀度显著升高(

0.01)；与模型组比较，霍山石斛高、中、低剂量组均可明显降低小鼠耳肿胀率(

0.01)；霍山石斛高、中剂量组足肿胀度明显降低(

0.05，

0.01)；给药各组棉球肉芽干重显著下降(

0.01)，胸腺、脾脏指数没有显著性变化；与正常组比较，模型组大鼠血清中炎症因子含量显著升高(

0.01)；与模型组比较，霍山石斛高、中、低剂量组和醋酸地塞米松组炎症因子含量显著降低(

0.05，

0.01)。

结论：

霍山石斛能够有效抑制急、慢性炎症反应。

Abstract

Objective:

To observe the anti-inflammatory effect of Dendrobii Huoshanense Herba.

Method:

Totally 60 Kunming mice were randomly divided into normal group

model group

high

middle and low-dose Dendrobii Huoshanense Herba groups (DHS-H

DHS-M

DHS-L

1 g·kg

-1

) and dexamethasone acetate group (DXMS

0.01 g·kg

-1

). The rats were intragastrically administered for 14 days. After the last administration for 1 h

a total of 20 μL of xylene was added to both sides of the right ear center of the mice to establish the ear swelling model. All of the mice were decapitated 1 h later

and the ear swelling inhibition rate of each group were calculated. Totally 36 healthy SD rats were divided into normal model

model group

DHS-H

DHS-M

DHS-L groups (2.8

1.4

0.7 g·kg

-1

) and DXMS acetate group. The rats were intragastrically administered for 7 days. One hour after the last intragastric administration

a foot swelling model was established through subcutaneous injection of 10%fresh egg white in the right hind limb toe of each group. The right hind paw circumference of each rat was measured at 0

0.5

5 h

and the paw swelling of the rats was calculated. Totally 60 SD rats were implanted subcutaneously with sterile dry cotton balls in the bilateral groin and grouped as above. All of the rats were intragastrically administered for 14 days since the next day. After the last administration

cotton balls were taken out

and the inhibition rate of granuloma was calculated. And the contents of tumor necrosis factor-

(TNF-

)

interleukin-1

(IL-1

)

interleukin-2 (IL-2)

interleukin-6 (IL-6)

interferon-

(IFN-

) in the serum of each group were detected.

Result:

Compared with the normal group

the ear swelling rate

the foot swelling degree were significantly increased in the model group(

0.01). Compared with the model group

the swelling rate of mouse ears in high

middle and low-dose Dendrobii Huoshanense Herba groups reduced significantly (

0.01). The swelling degrees of the high and middle-dose groups were significantly decreased (

0.05

0.01). The dry weight of cotton buds in each group was significantly decreased (

0.01)

with no significant change in thymus and spleen index. Compared with the normal group

the serum levels of TNF-

IL-1

IL-2

IL-6

and IFN-

in the model group were higher than those in the normal group (

0.01). And compared with model group

the levels of TNF-

IL-1

IL-2

IL-6

and IFN-

in Dendrobii Huoshanense Herba group and positive drug group were decreased significantly (

0.05

0.01).

Conclusion:

Dendrobii Huoshanense Herba could effectively inhibit acute and chronic inflammatory reactions.

关键词

Keywords

references

丁鸽，张代臻，丁小余．石斛资源分子水平研究进展 [J]．中国实验方剂学杂志， 2018 ， 24 ( 4 )： 208 - 215 ．

罗傲雪，宋关斌，淳泽，等．迭鞘石斛抗肿瘤作用研究 [J]．应用与环境生物学报， 2007 ， 13 ( 2 )： 184 - 187 ．

淳泽．药用石斛的资源危机与保护对策 [J]．资源开发与市场， 2005 ， 21 ( 2 )： 139 - 140 ．

刘守金．霍山石斛的本草考证 [J]．中药材， 1996 ， 19 ( 7 )： 373 - 375 ．

NIU Z , PAN J , XUE Q , et al . Plastome-wide comparison reveals new SNV resources for the authentication of and its corresponding medicinal slice (Huoshan Fengdou) [J]. Acta Pharm Sin B , 2018 , 8 : 466 - 477 .

WANG H , CHEN N F , ZHENG J Y , et al . Isolation and characterization of eleven polymorphic microsatellite loci for the valuable medicinal plant Dendrobii Huoshanense Herba and cross-species amplification [J]. Int J Mol Sci , 2012 , 13 : 16779 - 16784 .

谢冬．蓝莓与霍山石斛复方口服液的制备方法 [J]．园艺与种苗， 2018 ， 38 ( 10 )： 16 - 17 ．

HAO J W , CHEN N D , CHEN C W , et al . Rapid quantification of polysaccharide and the main onosaccharides in Dendrobii Huoshanense Herba by near-infrared attenuated total reflectance spectroscopy [J]. J Pharm Biomed Anal , 2018 , 151 : 331 - 338 .

SI H Y , CHEN N F , CHEN N D , et al . Structural characterisation of a water-soluble polysaccharide from tissue-cultured Dendrobii Huoshanense Herba C. Z.Tang et S. J.Cheng [J]. Nat Prod Res , 2018 , 32 : 252 - 260 .

王雅文，梁芷韵，谢镇山，等．铁皮石斛与霍山石斛中甘露糖、葡萄糖及柚皮素的含量比较 [J]．中国实验方剂学杂志， 2018 ， 24 ( 24 )： 1 - 8 ．

郝经文，王荣花，徐文冬，等．柱前衍生化HPLC同时测定霍山石斛中13种游离氨基酸含量 [J]．天然产物研究与开发， 2018 ， 30 ( 11 )： 1849 - 1857 ．

徐海军，方予，汪俊涛，等．霍山石斛多糖对小鼠的双向免疫调节作用 [J]．免疫学杂志， 2018 ， 34 ( 8 )： 731 - 736 ．

GE J C , ZHA X Q , NIE C Y , et al . Polysaccharides from Dendrobii Huoshanense Herba stems alleviates lung inflammation in cigarette smoke-induced mice [J]. Carbohydr Polym , 2018 , 189 : 289 - 295 .

谷仿丽，陈乃富，余茂耘，等．霍山石斛多糖对肥胖小鼠氧化应激的影响 [J]．皖西学院学报， 2016 ， 32 ( 5 )： 4 - 7 ．

ZHA X Q , DENG Y Y , LI X L , et al . The core structure of a Dendrobii Huoshanense Herba polysaccharide required for the inhibition of human lens epithelial cell apoptosis [J]. Carbohydr Polym , 2017 , 155 : 252 - 260 .

汪保英，王星，白明，等．不同产区连翘对小鼠急性炎症模型影响的研究 [J]．中医学报， 2011 ， 26 ( 9 )： 1070 - 1071 ．

袁岸，罗林，龚小红，等．连翘提取物对角叉菜胶和鸡蛋清所致大鼠足肿胀的影响研究 [J]．辽宁中医杂志， 2016 ， 43 ( 10 )： 2200 - 2203 ．

蔡博文．黑参糖浆的抗炎消肿实验研究 [J]．山西中医学院学报， 2015 ， 16 ( 5 )： 18 - 19 ．

苗光新，刘沛，毛晓霞，等．三黄颗粒与素代三黄颗粒对足跖肿胀和棉球肉芽肿模型的抗炎作用 [J]．承德医学院学报， 2015 ， 32 ( 1 )： 10 - 12 ．

坤舒．康颗粒抗大鼠棉球肉芽肿形成及其作用机制 [J]．中国医药导报， 2017 ， 14 ( 2 )： 12 - 15 ．

庞莹，魏俭铭，穆宏，等．降藓方治疗糜烂型口腔扁平苔藓阴虚内热证的疗效及对患者免疫功能、炎症因子的影响 [J]．河北中医， 2018 ， 40 ( 10 )： 1487 - 1490 ．

Hwang S Y , Kim J Y , Kim K W , et al . IL-17 induces production of IL-6 and IL-8 in rheumatoid arthritis synovial fibroblasts via NF-kappaB and PI3-kinase/Akt-dependent pathways [J]. Arthritis Res Ther , 2004 , 6 : R120 - R128 .

袁岸，罗林，龚小红，等．连翘提取物对角叉菜胶和鸡蛋清所致大鼠足肿胀的影响研究 [J]．辽宁中医杂志， 2016 ， 43 ( 10 )： 2200 - 2203 ．

包婷雯，左明丽，王敏，等．藏药麻花秦艽不同部位醇提物的抗炎作用研究 [J]．中国药房， 2018 ， 29 ( 22 )： 3114 - 3118 ．

Lee C T , KUO H C , CHEN Y H , et al . Current advances in the biological activity of polysaccharides in dendrobium with intriguing therapeutic potential [J]. Curr Med Chem , 2018 , 25 : 1663 - 1681 .

PAN L H , LI X F , WANG M N , et al . Comparison of hypoglycemic and antioxidative effects of polysaccharides from four different Dendrobium species [J]. Int J Biol Macromol , 2014 , 64 : 420 - 427 .

XIE S Z , HAO R , ZHA X Q , et al . Polysaccharide of Dendrobii Huoshanense Herba activates macrophages via toll-like receptor 4-mediated signaling pathways [J]. Carbohydr Polym , 2016 , 146 : 292 - 300 .

XIE S Z , LIU B , YE H Y , et al . Dendrobii Huoshanense Herba polysaccharide regionally regulates intestinal mucosal barrier function and intestinal microbiota in mice [J]. Carbohydr Polym , 2019 , 206 : 149 - 162 .

LI Q M , JIANG H , ZHA X Q , et al . Anti-inflammatory bibenzyls from the stems of Dendrobii Huoshanense Herba via bioassay guided isolation [J]. Nat Prod Res , 2018 , 16 : 1 - 4 .

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