Mechanism of Prunellae Spica on Non-small Cell Lung Cancer Based on Network Pharmacology

Yu-qing LIU; Ai-shuai WANG; Shan JIANG; Ze WANG; Xin ZHENG

doi:10.13422/j.cnki.syfjx.20192024

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Mechanism of Prunellae Spica on Non-small Cell Lung Cancer Based on Network Pharmacology

Data Mining | 更新时间：2021-02-09

- Mechanism of Prunellae Spica on Non-small Cell Lung Cancer Based on Network Pharmacology
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 24, Pages: 159-165(2019)
- 作者机构：
  
  山东中医药大学，济南　 250355
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20192024
  CLC： R22; R242; R2-031; R285.5
- Received：27 February 2019，
  
  Published Online：27 June 2019，
  
  Published：20 December 2019
- 稿件说明：
移动端阅览
Yu-qing LIU, Ai-shuai WANG, Shan JIANG, et al. Mechanism of Prunellae Spica on Non-small Cell Lung Cancer Based on Network Pharmacology[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(24): 159-165.
DOI：

Yu-qing LIU, Ai-shuai WANG, Shan JIANG, et al. Mechanism of Prunellae Spica on Non-small Cell Lung Cancer Based on Network Pharmacology[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(24): 159-165. DOI： 10.13422/j.cnki.syfjx.20192024.

摘要

目的：

基于网络药理学方法，探讨夏枯草治疗非小细胞肺癌的作用机制，阐述其科学内涵。

方法：

检索中草药系统药理学平台(TCMSP)获取夏枯草有效活性成分与作用靶点；检索疾病-基因组合数据库(DisGeNET)获取非小细胞肺癌相关疾病靶点；构建“药物-成分-疾病-靶点”网络，分析其作用机制；构建关键靶点蛋白质相互作用(PPI)网络，对关键靶点进行基因本体(GO)分类富集分析与京都基因与基因组百科全书(KEGG) 通路富集分析。

结果：

从TCMSP中获取有效活性成分11种，靶点178个；从DisGeNET数据库获取非小细胞肺癌相关基因2 019个；构建“药物-成分-疾病-靶点”网络，获得夏枯草治疗非小细胞肺癌的关键靶点114个；构建其PPI网络，包含节点114个，边1 952条。分析PPI网络，度值较高即夏枯草治疗非小细胞肺癌的关键靶点包括蛋白激酶B1(Akt1)，血管内皮生长因子(VEGFA)，半胱天冬酶-3(Caspase-3)，白细胞介素(IL)-6，JUN，丝裂原活化蛋白激酶(MAPK)8，原癌基因蛋白(MYC)，表皮生长因子受体(EGFR)等。对其进行生物学过程富集分析，显示涉及152种生物学过程，其中细胞增殖，细胞分裂，细胞凋亡等多种与癌症相关；KEGG富集涉及147条信号通路，其中磷脂酰肌醇-3激酶(PI3K)/Akt通路，核转录因子(NF)-

B通路，低氧诱导因子-1(HIF-1)通路，p53信号通路，EGFR信号通路等对非小细胞肺癌的发生、发展、侵袭、转移有着关键性作用是公认的。

结论：

本研究初步验证了夏枯草治疗NSCLC的基本药理学作用与机制，为进一步探究其作用机制奠定基础。

Abstract

Objective:

To explore the mechanism of Prunellae Spice in the treatment of non-small cell lung cancer (NSCLC) based on the network pharmacological method

in order to elaborate its scientific connotation.

Method:

The active ingredients and targets of Prunellae Spice were obtained through retrieval of the Traditional Chinese Medicine Integrated Database (TCMSP)

the disease-genome association database (DisGeNET) was searched to obtain the disease targets relating to non-small cell lung cancer

and the drug-component-disease-target network was constructed. The mechanism of action was analyzed. The key target protein-protein interaction (PPI) network was constructed. The gene ontology (GO) classification and enrichment analysis of key targets and the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis were carried out.

Result:

Totally 11 active ingredients and 178 targets were obtained from TCMSP

2019 genes relating to non-small cell lung cancer were obtained from DisGeNET database

114 key targets for Prunellae Spice to treat non-small cell lung cancer were obtained from drug-component-disease-target network

and 114 PPI networks were constructed

including 114 nodes and 1 952 edges. According to the analysis of PPI network

the key targets of Prunella vulgaris in the treatment of non-small cell lung cancer included protein kinase B (Akt)1

vascular endothelial growth factor(VEGFA)

Caspase-3

interleukin(IL)-6

JUN

mitogen-activated protein kinases(MAPK)8

MYC

epithelial growth factor receptor(EGFR). The enrichment of KEGG involved 152 biological processes

including cell proliferation

cell division

cell apoptosis. KEGG enrichment involved 147 signaling pathways. phosphoinositide 3-kinase (PI3K)/Akt pathway

NF-kappa B pathway

hypoxia inducible factor-1 (HIF-1) pathway

p53 signaling pathway and EGFR signaling pathway played key roles in the occurrence

development

invasion and metastasis of non-small cell lung cancer.

Conclusion:

This study preliminarily verified the basic pharmacological effect and mechanisms of Prunellae Spice on NSCLC

and laid a foundation for further exploring its mechanisms.

关键词

Keywords

references

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