Plasma Lipidomics of Coronary Heart Disease Patient with Syndrome of Intermingling of Phlegm and Static Blood Based on UPLC-Q/TOF-MS

Li-xia ZHU; Yuan-shi WEI; Xing-xing HUANG; Ying-feng ZHANG

doi:10.13422/j.cnki.syfjx.20192046

您当前的位置：

首页 >

文章列表页 >

Plasma Lipidomics of Coronary Heart Disease Patient with Syndrome of Intermingling of Phlegm and Static Blood Based on UPLC-Q/TOF-MS

Drug Metabolism | 更新时间：2021-02-09

- Plasma Lipidomics of Coronary Heart Disease Patient with Syndrome of Intermingling of Phlegm and Static Blood Based on UPLC-Q/TOF-MS
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 26, Issue 1, Pages: 110-117(2020)
- 作者机构：
  
  1.南方医科大学　珠江医院，广州　 510282
  2.广州中医药大学　中药学院，广州　 510006
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20192046
  CLC： R22; R24; R28; C37; R96
- Received：22 April 2019，
  
  Published Online：03 July 2019，
  
  Published：05 January 2020
- 稿件说明：
移动端阅览
Li-xia ZHU, Yuan-shi WEI, Xing-xing HUANG, et al. Plasma Lipidomics of Coronary Heart Disease Patient with Syndrome of Intermingling of Phlegm and Static Blood Based on UPLC-Q/TOF-MS[J]. Chinese journal of experimental traditional medical formulae, 2020, 26(1): 110-117.
DOI：

Li-xia ZHU, Yuan-shi WEI, Xing-xing HUANG, et al. Plasma Lipidomics of Coronary Heart Disease Patient with Syndrome of Intermingling of Phlegm and Static Blood Based on UPLC-Q/TOF-MS[J]. Chinese journal of experimental traditional medical formulae, 2020, 26(1): 110-117. DOI： 10.13422/j.cnki.syfjx.20192046.

摘要

目的：

研究痰瘀互结型冠心病患者的血浆脂质组学特征，寻找其与健康志愿者之间的差异脂质代谢物。

方法：

采集痰瘀互结型冠心病患者及同年龄段健康志愿者血浆，采用UPLC-Q/TOF-MS进行血浆脂质组学研究。血浆样本在正、负离子模式下分别进行检测，同时采集一级和二级质谱(

检测范围分别为100～2 000，50～2 000)，采用正交偏最小二乘法-判别分析(OPLS-DA)建立脂质组学模型，基于多元统计发现并鉴定差异脂质代谢物。

结果：

OPLS-DA模型可明显区分痰瘀互结型冠心病患者及健康志愿者，发现并鉴定了15个差异脂质代谢物，分别为C16 sphinganine，植物鞘氨醇，

，

-dimethyl-safingol，2-hydroxyphytanic acid，orotinichalcone，PC[18∶2(2

，4

)/0∶0]，PC(0∶0/16∶0)，epitestosterone sulfate，etiocholanolone sulfate，PS[22∶1(11

)/0∶0]，PC[16∶0/20∶4(5

，8

，11

，14

)]，PC[19∶1(9

)/17∶2(9

，12

)]，PC(16∶0/0∶0)，PC(18∶0/0∶0)，PS[15∶1(9

)/22∶1(11

)]。

结论：

痰瘀互结型冠心病患者及健康志愿者的血浆脂质组学特征存在显著差异，血浆差异脂质代谢物有助于痰瘀互结型冠心病的的准确辨证。

Abstract

Objective:

To study on the plasma lipidomics characteristics of coronary heart disease (CHD) patients with syndrome of of intermingling of phlegm and static blood

and to find differential lipid metabolites between them and healthy volunteers.

Method:

The plasma samples from CHD patients with syndrome of intermingling of phlegm and static blood and healthy volunteers of the same age were collected. The plasma lipidomics was carried out by UPLC-Q/TOF-MS. The plasma samples were detected under positive and negative ion modes

and the primary and secondary mass spectrometry datas were collected simultaneously

and the

ranges were 100-2 000 and 50-2 000

respectively. The lipidomics model was established by orthogonal partial least squares discriminant analysis (OPLS-DA). Differential lipid metabolites were identified based on multivariate statistics.

Result:

OPLS-DA model could obviously distinguish CHD patients with syndrome of intermingling of phlegm and static blood and healthy volunteers. A total of 15 plasma differential lipid metabolites were identified

such as C16 sphinganine

phytosphingosine

-dimethyl-safingol

2-hydroxyphytanic acid

orotinichalcone

PC[18∶2(2

)/0∶0]

PC(0∶0/16∶0)

epitestosterone sulfate

etiocholanolone sulfate

PS[22∶1(11

)/0∶0]

PC[16∶0/20∶4(5

)]

PC[19∶1(9

)/17∶2(9

)]

PC(16∶0/0∶0)

PC(18∶0/0∶0)

PS[15∶1(9

)/22∶1(11

)].

Conclusion:

There are significant differences in plasma lipid characteristics between CHD patients with syndrome of intermingling of phlegm and static blood and healthy volunteers. The plasma differential lipid metabolites are helpful for the accurate differentiation of CHD patients with syndrome of intermingling of phlegm and static blood.

关键词

Keywords

references

闫丹丹，边育红，于春泉．冠心病痰瘀互结病机及生物学基础研究概述 [J]．天津中医药大学学报， 2018 ， 37 ( 1 )： 1 - 5 .

李先涛．对冠心病痰瘀互结证(胸痹心痛)临床辨证的认识 [J]. 辽宁中医杂志， 2016 ， 43 ( 1 )： 10 - 12 .

A Shevchenko ， K Simons ． Lipidomics：coming to grips with lipid diversity [J]． Nat Rev Mol Cell Biol ， 2010 ， 11 ( 8 )： 593 - 598 .

C HU ， R van der Heijden ， M WANG ， et al . Analytical strategies in lipidomics and applications in disease biomarker discovery [J]． J Chromatogr B Analyt Technol Biomed Life Sci ， 2009 ， 877 ( 26 )： 2836 - 2846 .

X LIU ， J LI ， P ZHENG ， et al . Plasma lipidomics reveals potential lipid markers of major depressive disorder [J]． Anal Bioanal Chem ， 2016 ， 408 ( 23 )： 6497 - 6507 .

X HAN ， R W Gross ． Global analyses of cellular lipidomes directly from crude extracts of biological samples by electrospray ionization mass spectrometry：a bridge to lipidomics [J]． J Lipid Res ， 2003 ， 44 ( 6 )： 1071 - 1079 .

杜丽娜，单进军，谢彤，等．呼吸系统疾病相关脂类及脂质组学研究进展 [J]．中国实验方剂学杂志， 2015 ， 21 ( 4 )： 219 - 223 .

赵铁，张浩军，殷婷婷，等．柴黄益肾颗粒对糖尿病肾病大鼠保护作用的脂质组学研究 [J]．中国实验方剂学杂志， 2012 ， 18 ( 15 )： 155 - 162 .

E G Bligh ， W J Dyer ． A rapid method of total lipid extraction and purification [J]． Can J Biochem Physiol ， 1959 ， 37 ( 8 )： 911 - 917 .

K Nagano ， H Kano ， H Arito ， et al . Enhancement of renal carcinogenicity by combined inhalation and oral exposures to chloroform in male rats [J]． J Toxicol Environ Health A ， 2006 ， 69 ( 20 )： 1827 - 1842 .

S K Byeon ， J Y Lee ， M H Moon ． Optimized extraction of phospholipids and lysophospholipids for nanoflow liquid chromatography-electrospray ionization-tandem mass spectrometry [J]． Analyst ， 2012 ， 137 ( 2 )： 451 - 458 .

H M Myeong ． Phospholipid analysis by nanoflow liquid chromatography-tandem mass spectrometry [J]． Mass Spectrom Lett ， 2014 ， 5 ( 1 )： 1 - 11 .

Z WANG , E Klipfell , B J Bennett ， et al . Gut flora metabolism of phosphatidylcholine promotes cardiovascular disease [J]． Nature ， 2011 ， 472 ( 7341 )： 57 - 63 .

魏晓东，李莉，白晶．复方丹参对冠心病患者红细胞膜磷脂成分的影响 [J]．中国中西医结合杂志， 1997 ， 17 ( 6 )： 336 - 338 .

A Ganna ， S Salihovic ， J Sundström ， et al . Large-scale metabolomic profiling identifies novel biomarkers for incident coronary heart disease [J]． PLoS Genet ， 2014 ， 10 ( 12 )： e1004801 .

Y T LIU ， H M JIA ， X CHANG ， et al . Metabolic pathways involved in Xin-Ke-Shu protecting against myocardial infarction in rats using ultra high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry [J]． J Pharm Biomed Anal ， 2014 ， 90 ： 35 - 44 .

A J Greenberg ， S R Hackett ， L G Harshman ， et al . Environmental and genetic perturbations reveal different networks of metabolic regulation [J]． Mol Syst Biol ， 2011 ， 7 ： 563 .

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Establishment and Evaluation of Rat Model of Myocardial Ischemia-reperfusion Injury with Phlegm and Blood Stasis Blocking Collaterals Syndrome Based on Metabolomics

Establishment and Evaluation of Rat Model of Acute Myocardial Infarction in Coronary Heart Disease with Qi and Yin Deficiency Syndrome Based on Sleep Deprivation Combined with Coronary Artery Ligation

Mechanism of Xuefu Zhuyutang in Intervening in Ferroptosis in Rats with Coronary Heart Disease with Blood Stasis Syndrome Based on ACSL4 Signalling Pathway

Exploring Therapeutic Effect of Yuejuwan on Depressed Mice Based on Lipidomics

Identification of Pharmacodynamic Material Basis of Ruyi Zhenbaowan by Multidimensional Correlation Model of "Pharmacodynamic-target-component-pharmacokinetic"

Related Author

HU Longxiao

GAO Jiabei

MA Weihao

LU Jieming

GAO Yunxiao

YUAN Yue

ZHANG Qiuyan

CHEN Xiaoxiao

Related Institution

School of Clinical Medicine，Beijing University of Chinese Medicine

Beijing Key Laboratory of Chinese Materia Pharmacology，National Clinical Research Center of Traditional Chinese Medicine for Cardiovascular Diseases，Institute of Basic Medical Sciences， Xiyuan Hospital，China Academy of Chinese Medical Sciences

Natural History Museum of China

Institute of Basic Medical Sciences，Xiyuan Hospital，China Academy of Chinese Medical Sciences

Hunan University of Chinese Medicine

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰