Effect of Menthol on Proliferation, Migration and Expressions of IL-8, CXCL-12 and VEGF in Hepatoma HepG2 Cells

Xing-kui TAO; Xing-tao ZHANG; Hai-chao WANG; Hong DUAN; Jin CHENG

doi:10.13422/j.cnki.syfjx.20192124

您当前的位置：

首页 >

文章列表页 >

Effect of Menthol on Proliferation, Migration and Expressions of IL-8, CXCL-12 and VEGF in Hepatoma HepG2 Cells

Pharmacology | 更新时间：2021-02-09

- Effect of Menthol on Proliferation, Migration and Expressions of IL-8, CXCL-12 and VEGF in Hepatoma HepG2 Cells
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 21, Pages: 60-65(2019)
- 作者机构：
  
  宿州学院　生物与食品工程学院，宿州市天然产物与功能性食品工程技术研究中心，安徽　宿州　 234000
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20192124
  CLC： R22; R242; R2-031;R285.5
- Received：31 May 2019，
  
  Published Online：17 July 2019，
  
  Published：05 November 2019
- 稿件说明：
移动端阅览
Xing-kui TAO, Xing-tao ZHANG, Hai-chao WANG, et al. Effect of Menthol on Proliferation, Migration and Expressions of IL-8, CXCL-12 and VEGF in Hepatoma HepG2 Cells[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(21): 60-65.
DOI：

Xing-kui TAO, Xing-tao ZHANG, Hai-chao WANG, et al. Effect of Menthol on Proliferation, Migration and Expressions of IL-8, CXCL-12 and VEGF in Hepatoma HepG2 Cells[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(21): 60-65. DOI： 10.13422/j.cnki.syfjx.20192124.

摘要

目的：

体外实验观察不同剂量薄荷醇对肝癌HepG2细胞增殖、迁移及白细胞介素-8(IL-8)，趋化因子-12(CXCL-12)和血管内皮生长因子(VEGF)表达的影响，阐释薄荷醇抗肝癌的作用及其相关机制。

方法：

设立薄荷醇(25，50，100，200 μmol·L

－1

)组以及空白组作用于肝癌HepG2细胞，利用细胞增殖与毒性检测(CCK-8)法和5-乙炔基-2′脱氧尿嘧啶核苷(EDU)检测方法检测薄荷醇对HepG2细胞增殖的影响，利用迁移实验(Transwell)检测薄荷醇对HepG2细胞迁移能力的影响，利用实时荧光定量聚合酶链式反应检测薄荷醇处理HepG2细胞炎症和趋化因子IL-8，CXCL-12 mRNA表达水平，通过蛋白免疫印迹法(Western blot)检测薄荷醇处理对HepG2细胞中VEGF蛋白的表达水平。

结果：

与空白组比较，薄荷醇(25，50，100，200 μmol·L

－1

)在体外对HepG2细胞增殖和迁移均有抑制作用。薄荷醇100 μmol·L

－1

时抑制作用明显(

0.05)；薄荷醇(50，100，200 μmol·L

－1

)对HepG2细胞迁移能力有明显抑制作用(

0.05)，呈剂量依赖性。与空白组比较，薄荷醇(100，200 μmol·L

－1

)均能显著抑制HepG2细胞中的IL-8，CXCL-12 mRNA和VEGF蛋白的表达水平(

0.05)，进一步证实薄荷醇通过抑制炎症趋化因子起到抗癌作用。

结论：

薄荷醇在体外对肝癌HepG2细胞增殖、迁移具有明显抑制作用，潜在机制可能与其抑制细胞IL-8，CXCL-12，VEGF的表达有关。此结论为薄荷醇抗肝癌作用的阐释提供了实验依据。

Abstract

Objective:

To study the effect of menthol on proliferation

migration and expressions of interleukin-8(IL-8)

C-X-C motif chemokine-12(CXCL-12) and vascular endothelial growth factor(VEGF) in hepatoma HepG2 cells

in vitro

in order to elucidate the anti-liver cancer activity of menthol and relevant mechanisms.

Method:

Different concentrations of menthol (25

100

200 μmol·L

-1

) and blank group were applied to Hepatoma HepG2 cells. Cell counting kit-8(CCK-8) and EDU were used to detect the proliferation effect of menthol on HepG2 cell. Transwell experiment was used to detect the migration effect of menthol on HepG2 cell. Real-time fluorescent quantitative polymerase chain reaction technique was used to detect the inflammatory chemokines IL-8 and CXCL-12 mRNA expression levels in HepG2 cell. Western blot was used to detect the expression level of VEGF in HepG2 cells treated with menthol.

Result:

Compare with the blank group

menthol (25

100

200 μmol·L

-1

) significantly inhibited proliferation and migration of HepG2 cells

in vitro

. When the concentration of menthol was 100 μmol·L

-1

microns

the inhibitory effect was significant (

0.05). Menthol significantly inhibited the migration of HepG2 cells. The higher the concentration of menthol was

the less the number of cell migration was. Compared with the blank group

menthol (100

200 μmol·L

-1

) significantly inhibited the expression levels of IL-8

CXCL-12 mRNA and VEGF protein in HepG2 cells (

0.05)

indicating that menthol is an anticancer agent by inhibiting inflammation and chemokines.

Conclusion:

Menthol has an inhibitory effect on the proliferation and migration of hepatoma HepG2 cells

in vitro

and the potential anti-liver cancer mechanism might be related to the inhibition of IL-8

CXCL-12 and VEGF expressions in the cells. This conclusion provides the experimental basis for elucidating the effect of menthol against liver cancer.

关键词

Keywords

references

陈万青，李霓，石菊芳，等．中国城市癌症早诊早治项目进展 [J]．中国肿瘤， 2019 ， 28 ( 1 )： 23 - 25 ．

靳秀丽，李异玲． lncRNA与肝癌相关性研究进展 [J]．胃肠病学和肝病学杂志， 2019 ， 28 ( 3 )： 345 - 349 ．

黄硕，叶娜妮，张依静，等．何若苹辨治原发性肝癌经验 [J]．中医杂志， 2019 ， 60 ( 9 )： 737 - 740 ．

朱丽娜，谢宝杰，殷风华，等． DynaCT对肝转移瘤介入治疗具有指导意义 [J]．基因组学与应用生物学， 2019 ， 38 ( 5 )： 2265 - 2269 ．

万思哲，朱萱． RhoA对肝癌进展的调节作用机制 [J]．中国生物化学与分子生物学报， 2019 ， 35 ( 5 )： 480 - 485 ．

张晶晶，金小宝，李小波，等．过表达Glypican-3对高转移性肝癌细胞HCCLM3生物学行为的影响 [J]．生物技术通报， 2017 ， 33 ( 12 )： 176 - 184 ．

于清跃，朱新宝．薄荷种植与薄荷精油提取研究进展 [J]．安徽农业科学， 2012 ， 40 ( 13 )： 7911 - 7913 ．

Oz M , El Nebrisi E G , Yang K H S , et al . Cellular and molecular targets of menthol actions [J]. Front Pharmacol , 2017 , 8 : 472 .

Nanda Prasad S V , Reddy A Y , Nonitha S . Effects of menthol-flavored substances at the cellular level on oral mucosal sites [J]. Dent Res J , 2019 , 16 ( 1 ): 7 - 11 .

Yee N . Roles of TRPM8 ion channels in cancer: proliferation, survival, and invasion [J]. Cancers , 2015 , 7 ( 4 ): 2134 - 2146 .

LIU Z , SHEN C , TAO Y , et al . Chemopreventive efficacy of menthol on carcinogen-induced cutaneous carcinoma through inhibition of inflammation and oxidative stress in mice [J]. Food Chem Toxicol , 2015 , 82 : 12 - 18 .

陈晨，刘兆国，汪思亮，等．薄荷醇及其受体TRPM8与肿瘤关系研究进展 [J]．中国药理学通报， 2015 ， 31 ( 3 )： 312 - 314 ．

苗延青，梁静，刘漫．黄芩苷协调大黄素薄荷醇对胃癌SGC-7901细胞增殖抑制作用的研究 [J]．山西医药杂志， 2019 ， 48 ( 8 )： 888 - 890 ．

于文建，王磊，韩利平，等．雄激素非依赖性前列腺癌DU145细胞中TRPM8和TRPA1的表达及活性研究 [J]．癌症进展， 2017 ， 15 ( 7 )： 754 - 758 ．

肖潺潺，陈茂剑，梅凡彪，等．辣椒碱对肝癌SMMC-7721细胞迁移和侵袭的影响及机制 [J]．中国实验方剂学杂志， 2018 ， 24 ( 18 )： 124 - 129 ．

刘婷，丁艳，杨婷婷，等．鲜枸杞子提取物通过p38 MAPK信号通路抑制人肝癌细胞HepG2诱导小鼠恶病质的作用及机制 [J]．中国实验方剂学杂志， 2019 ， 25 ( 9 )： 89 - 94 ．

郑雪莹，曹宁宁，裴帅，等．人参皂苷Rh2抗肝癌作用机制的研究进展 [J]．中国实验方剂学杂志， 2019 ， doi: 10.13422/j.cnki.syfjx.20191329 http://doi.org/10.13422/j.cnki.syfjx.20191329 ．

黎勤．薄荷醇通过TRPM8通道诱导人膀胱癌T24细胞株死亡的试验研究 [D]．广州：南方医科大学， 2010 ．

刘雷，杨立业． EdU在检测细胞增殖中的应用 [J]．医学综述， 2010 ， 16 ( 19 )： 2901 - 2904 ．

郭苇，谭为，王昌俊． miRNA 与肝癌的关系 [J]．广州中医药大学学报， 2015 ， 32 ( 3 )： 185 - 188 ．

LIU Z G , SHEN X S , TAO Y , et al . Chemopreventive efficacy of menthol on carcinogen-induced cutaneous carcinoma through inhibition of inflammation and oxidative stress in mice [J]. Food Chem Toxicol , 2015 , 82 : 12 - 18 .

LIU B , FAN L , Balakrishna S , et al . TRPM8 is the principal mediator of menthol-induced analgesia of acute and inflammatory pain [J]. Pain , 2013 , 154 ( 10 ): 2169 - 2177 .

Domanska U M , Kruizinga R C , Nagenagast W B , et al . A review on CXCR4/CXCL12 axis in oncology: no place to hide [J]. Eur J Cancer , 2013 , 49 : 219 - 230 .

李思睿，刘阳，李果，等． CXCR4/CXCL12轴与肿瘤发生发展中的作用研究进展 [J]．生理科学进展， 2018 ， 49 ( 5 )： 375 - 378 ．

杨敏，傅海涛，杨再兴，等． IL-8及其受体CXCR1、CXCR2与肝癌关系的研究进展 [J]．临床检验杂志， 2013 ， 31 ( 12 )： 926 - 928 ．

Akiba J , Yano H , Ogasawara S , et al . Expression and function of interleukin-8 in human hepatocellular carcinoma [J]. Int J Oncol , 2001 , 18 ( 2 ): 257 - 264 .

Gentilini A , Rombouts K , Galastri S , et al . Role of the stromal-derived factor-1 CXCR4 axis in the interaction between hepatic stellate cells and cholangiocarcinoma [J]. J Hepatol , 2012 , 57 ( 4 ): 813 - 820 .

施国英，施德金，吕伟标，等．基质细胞衍化生长因子-1及其受体CXCR4在肝细胞癌和肝硬化中的表达 [J]．中华肝脏病杂志， 2007 ， 15 ( 4 )： 276 - 278 ．

Mohamed A , Chenna A , Abdelfatah M , et al . Microvessel density analysis in patients with viral hepatitis-related hepatocellular carcinoma [J]. J Gastrointest Cancer , 2015 , 46 ( 2 ): 104 - 108 .

Kasprzak A , Surdacka A , Tomczak M , et al . Expression of ngiogenesis-stimulating factors(VEGF, CD31, CD105) and angiogenetic index in gingivae of patients with chronic periodontitis [J]. Folia Histochem Cytobiol , 2012 , 50 ( 4 ): 554 - 564 .

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Effect of Oxymatrine in Inhibiting Proliferation and Migration of HT-29 Cells Induced by Insulin

Effect of Sijunzi Tang in Promoting Migration and Proliferation of Small Intestinal Epithelial Cells by Up-Regulating c-Myc Expression

Molecular Mechanism of Gypenoside L in Anti-Ovarian Cancer by Affecting GCK-Mediated Glycolytic Pathway

Traditional Chinese Medicine Regulates JAK/STAT Signaling Pathway to Treat Colorectal Cancer： A Review

Effect of Mahuang Xixin Fuzitang on Migration of Dendritic Cells in Mice by Regulating Rho/ROCK Signaling Pathway

Related Author

Di PAN

Wen ZHANG

Rong ZHANG

Shi-quan GAN

Yan CHEN

Nen-ling ZHANG

Yi-ni XU

Xiang-chun SHEN

Related Institution

Key Laboratory of Optimal Utilization of Natural Medicine Resources, Guizhou Medical University

Guizhou Province Natural Medicinal Resources High-Efficacy Application Engineering Center

Liaoning Provincial Hospital of TCM

Liaoning University of Traditional Chinese Medicine（TCM）

Lu'an Hospital of Traditional Chinese Medicine Affiliated to Anhui University of Chinese Medicine

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰