Evaluation of Different Effect of Pith-nodecayed and Pith-decayed Products of Scutellariae Radix on Large Intestine Damp-heat Syndrome Based on Pharmacological Indexes

Fang-lei ZHANG; Jia-wen ZHAO; Jiang-hui HU; Kun-yu XIAO; Ying XIE; Lin LEI; Yan-ju LIU

doi:10.13422/j.cnki.syfjx.20192154

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Evaluation of Different Effect of Pith-nodecayed and Pith-decayed Products of Scutellariae Radix on Large Intestine Damp-heat Syndrome Based on Pharmacological Indexes

Pharmacology | 更新时间：2021-02-09

- Evaluation of Different Effect of Pith-nodecayed and Pith-decayed Products of Scutellariae Radix on Large Intestine Damp-heat Syndrome Based on Pharmacological Indexes
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 26, Issue 7, Pages: 57-64(2020)
- 作者机构：
  
  湖北中医药大学　药学院，湖北省中药炮制技术工程研究中心，武汉　 430065
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20192154
  CLC： R22; R24; R28; C37; TS193; R96
- Received：29 April 2019，
  
  Published Online：19 July 2019，
  
  Published：05 April 2020
- 稿件说明：
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Fang-lei ZHANG, Jia-wen ZHAO, Jiang-hui HU, et al. Evaluation of Different Effect of Pith-nodecayed and Pith-decayed Products of Scutellariae Radix on Large Intestine Damp-heat Syndrome Based on Pharmacological Indexes[J]. Chinese journal of experimental traditional medical formulae, 2020, 26(7): 57-64.
DOI：

Fang-lei ZHANG, Jia-wen ZHAO, Jiang-hui HU, et al. Evaluation of Different Effect of Pith-nodecayed and Pith-decayed Products of Scutellariae Radix on Large Intestine Damp-heat Syndrome Based on Pharmacological Indexes[J]. Chinese journal of experimental traditional medical formulae, 2020, 26(7): 57-64. DOI： 10.13422/j.cnki.syfjx.20192154.

摘要

目的：

比较子芩与枯芩对大鼠大肠湿热证的药效差异，通过现代药理实验验证将黄芩分为子芩与枯芩分别入药的科学性。

方法：

将大鼠随机分为空白组，模型组，子芩低、高剂量组(0.9，3.6 g·kg

-1

)，黄芩组(0.9 g·kg

-1

)，枯芩低、高剂量组(0.9，3.6 g·kg

-1

)，复方黄连素片组(0.045 g·kg

-1

)，每组8只。选择大肠湿热证大鼠模型，选择体温、胸腺指数、脾脏指数、回肠病理切片、结肠病理切片、炎症因子以及分泌型免疫球蛋白(SIg)A含量为指标评价子芩与枯芩对大肠湿热证的治疗作用，综合评价子芩与枯芩的药效差异。采用偏最小二乘法-判别分析(PLS-DA)对2种饮片抗大肠湿热证药理指标进行统计学分析。

结果：

不同剂量的子芩和枯芩均能降低大肠湿热证模型大鼠的体温、胸腺指数和脾脏指数、血清炎症因子白细胞介素(IL)-2，IL-6，IL-1

和肠黏膜SIgA含量，且大部分具有显著性差异(

0.05，

0.01)；与等剂量枯芩组比较，相应剂量子芩组效果更好，且大部分具有显著性差异(

0.05，

0.01)。PLS-DA结果显示子芩与枯芩各聚类于一侧。

结论：

子芩与枯芩对大肠湿热证均具有治疗作用，但两者的作用强度有明显差异，子芩优于枯芩，验证了古方中用子芩专泻大小肠下焦之火的科学性。

Abstract

Objective:

To compare the pharmacodynamic effects of pith-nodecayed and pith-decayed products of Scutellariae Radix on rats with large intestine damp-heat syndrome

and to demonstrate the scientificness of dividing Scutellariae Radix into pith-nodecayed and pith-decayed products as medicines by modern pharmacological test.

Method:

Rats were randomly divided into blank group

model group

low-and high-dose group of pith-nodecayed products (0.9

3.6 g·kg

-1

)

low-and high-dose group of pith-decayed products (0.9

3.6 g·kg

-1

)

Scutellariae Radix group (0.9 g·kg

-1

)

compound berberine tablets group (positive drug group

0.045 g·kg

-1

)

and 8 rats in each group. Taking model rats with large intestine damp-heat syndrome

the body temperature

thymus index

spleen index

pathological sections of colon and ileum

inflammatory factors and Secretory immunoglobulin (SIg) A content were selected as indexes to evaluate the therapeutic effect of pith-nodecayed and pith-decayed products on large intestine damp-heat syndrome

and make comprehensive evaluation of the difference in efficacy between them. Partial least squares-discriminant analysis (PLS-DA) was employed to analyze the pharmacological indexes of these two products against large intestine damp-heat syndrome.

Result:

Pith-nodecayed and pith-decayed products of Scutellariae Radix with different doses could reduce the body temperature

thymus index

spleen index

contents of interleukin (IL)-2

IL-6

IL-1

in serum and SIgA content in intestinal mucosa

and most of them had significant differences (

0.05

0.01). Compared with the isodose group of pith-decayed products

the effect of corresponding dose group of pith-nodecayed products was better

and most of them had significant differences (

0.05

0.01). PLS-DA results indicated that there were significant differences in the pharmacological effects of pith-nodecayed and pith-decayed products

and they were clustered on one side

respectively.

Conclusion:

Both of pith-nodecayed and pith-decayed products of Scutellariae Radix have therapeutic effect on large intestine damp-heat syndrome with distinctly different strength of action

and pith-nodecayed products is superior to pith-decayed products

which verify the scientific nature of pith-nodecayed products was specializedly used to treat bowel disease in ancient times.

关键词

Keywords

references

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