Effect of Buyang Huanwu Tang on Synaptic Structural Plasticity After Cerebral Ischemia-reperfusion in Rats

Kai-ling YANG; Ying ZHOU; Fu-man YAN; Le-quan ZHOU; Yu-lian LIU; Wei LIU

doi:10.13422/j.cnki.syfjx.20192201

您当前的位置：

首页 >

文章列表页 >

Effect of Buyang Huanwu Tang on Synaptic Structural Plasticity After Cerebral Ischemia-reperfusion in Rats

Classic Prescriptions | 更新时间：2021-02-09

- Effect of Buyang Huanwu Tang on Synaptic Structural Plasticity After Cerebral Ischemia-reperfusion in Rats
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 26, Issue 1, Pages: 43-49(2020)
- 作者机构：
  
  广州中医药大学　基础医学院，广州　 510006
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20192201
  CLC： R2-0; R22; R285.5; R289
- Received：15 April 2019，
  
  Published Online：07 August 2019，
  
  Published：05 January 2020
- 稿件说明：
移动端阅览
Kai-ling YANG, Ying ZHOU, Fu-man YAN, et al. Effect of Buyang Huanwu Tang on Synaptic Structural Plasticity After Cerebral Ischemia-reperfusion in Rats[J]. Chinese journal of experimental traditional medical formulae, 2020, 26(1): 43-49.
DOI：

Kai-ling YANG, Ying ZHOU, Fu-man YAN, et al. Effect of Buyang Huanwu Tang on Synaptic Structural Plasticity After Cerebral Ischemia-reperfusion in Rats[J]. Chinese journal of experimental traditional medical formulae, 2020, 26(1): 43-49. DOI： 10.13422/j.cnki.syfjx.20192201.

摘要

目的：

探讨补阳还五汤在脑缺血再灌注大鼠恢复期提高突触可塑性的机制研究。

方法：

建立大脑中动脉栓塞(MCAO)大鼠模型，随机分为假手术组、模型组、补阳还五汤组、补阳还五汤联合缝隙连接蛋白43(Cx43)抑制剂(Gap26)组，补阳还五汤每天灌胃2次(16 g·kg

-1

)，Gap26于术后第3天腹腔注射，每天1次(25 μg·kg

-1

)；7 d后取材，采用透射电镜观察缺血侧海马突触和缝隙连接超微结构的改变，运用蛋白免疫印迹法(Western blot)和免疫荧光检测缺血侧海马突触素(SYN)，生长相关蛋白-43(GAP-43)的表达。

结果：

电镜下观察到假手术组突触结构完整、清晰，突触数量多，缝隙连接结构清晰；模型组缺血侧海马突触结构溶解，突触数量减少，缝隙连接消失，存在较大间隙，与假手术组比较，SYN，GAP-43的表达明显增高(

0.05，

0.01)；补阳还五汤组缺血侧海马突触结构较清晰，突触数量增多，缝隙连接结构较完整，与模型组比较，SYN，GAP-43的表达明显增强(

0.05，

0.01)；而联合使用Gap26后缺血侧海马突触数量较补阳还五汤组减少，仅可见少量结构完整的缝隙连接，补阳还五汤增强SYN，GAP-43的作用被明显抑制(

0.05，

0.01)。

结论：

补阳还五汤可提高脑缺血再灌注恢复期缺血侧海马突触可塑性，其机制可能与增加Cx43的表达促进对SYN，GAP-43的干预有关。

Abstract

Objective:

To investigate the mechanism of Buyang Huanwu Tang (BYHWT) in improving synaptic structural plasticity after cerebral ischemia-reperfusion in rats.

Method:

Middle cerebral artery occlusion and reperfusion model was established. SD rats were randomly divided into sham-operated group

model group

BYHWT group

BYHWT+ Gap26(connexin43 inhibitor)groups. BYHWT was given twice a day(16 g·kg

-1

)

Gap26 was intraperitoneally injected once a day since the third day after surgery (25 g·kg

-1

). Brain was taken out at the 7

day. The changes of neuronal synaptic and gap junction ultrastructure were observed by transmission electron microscopy. Synaptophysin (SYN) and growth-associated protein-43 (GAP-43) protein expression were detected by Western blot and immunofluorescence.

Result:

The structure of synapses was integrated

and the gap junctions were clear in sham-operated group. In the hippocampus of model group

the structure was destroyed

and the gap junctions disappeared. Compared with the sham-operated group

model group up-regulated the expressions of SYN and GAP-43 (

0.05

0.01). In the hippocampus of BYHWT group

the structure was close to the normal. Furthermore

BYHWT up-regulated the expressions of SYN and GAP-43 (

0.05

0.01). However

after the combined administration with Cx43 inhibitor (Gap26)

the damage of synaptic structural decreased

only a small number of gap junctions with the structural integrity can be seen

and the effect of BYHWT on SYN and GAP-43 was inhibited (

0.05

0.01).

Conclusion:

BYHWT could improve the hippocampal synaptic structural plasticity obviously after the CIRI. The mechanism may be related to the increase of the expression of Cx43 and the promotion of the intervention of SYN and GAP-43.

关键词

Keywords

references

H J CHEN ， Y C SHEN ， Y J SHIAO ， et al . Multiplex brain proteomic analysis revealed the molecular therapeutic effects of buyang huanwu decoction on cerebral ischemic stroke mice [J]. PLoS One ， 2015 ， 10 ( 10 )： e0140823 .

W W ZHANG ， F XU ， D WANG ， et al . Buyang Huanwu Decoction ameliorates ischemic stroke by modulating multiple targets with multiple components: in vitro evidences [J]. Chin J Nat Med ， 2018 ， 16 ( 3 )： 194 - 202 .

吴玉芙，刘晓红，郭伟成，等．补阳还五汤对气虚血瘀型脑梗死患者脑血管储备功能的影响 [J]．中国实验方剂学杂志， 2017 ， 23 ( 12 )： 162 - 167 .

P YU ， L GUAN ， L ZHOU ， et al . Upregulation of glutamate metabolism by BYHWT in cultured astrocytes following oxygen-glucose deprivation/reoxygenation in part depends on the activation of p38 MAPK [J]. Exp Ther Med ， 2017 ， 13 ( 6 )： 3089 - 3096 .

B LIU ， G CAI ， J YI . Buyang Huanwu Decoction regulates neural stem cell behavior in ischemic brain [J]. Neural Regen Res ， 2013 ， 8 ( 25 )： 2336 - 2342 .

丘敏，曾维勇，刘微，等．补阳还五汤对脑缺血再灌注后星形胶质细胞connexin43影响 [J]．辽宁中医药大学学报， 2015 ， 17 ( 5 )： 36 - 38 .

刘微，郭建超，苏宁，等．补阳还五汤通过缝隙连接蛋白43抗脑缺血损伤 [J]．中华中医药学刊， 2018 ， 36 ( 9 )： 2068 - 2070 .

E Z Longa ， P R Weinstein ， S Carlson . Reversible middle cerebral artery occlusion without craniectomy in rats [J]． Stroke ， 1989 ， 20 ( 1 )： 84 - 91 .

李晓静 . 抑制缝隙连接蛋白43促进新生大鼠脑缺血损伤后神经功能的恢复 [D]．苏州：苏州大学， 2015 .

N Rouach ， A Koulakoff ， V Abudara ， et al . Astroglial metabolic networks sustain hippocampal synaptic transmission [J]. Science ， 2008 ， 322 ( 5907 )： 1551 - 1555 .

D V Krysko ， L Leybaert ， P Vandenabeele . Gap junctions and the propagation of cell survival and cell death signals [J]. Apoptosis ， 2005 ， 10 ( 3 )： 459 - 469 .

朱丽萍 . Cx43及Cx43-hemichannel在大鼠全脑缺血再灌注损伤中的作用 [D]．济南：山东大学， 2012 .

朱原，蔡俊，徐愉林，等．补阳还五汤对脑缺血再灌注损伤大鼠PTEN mRNA的影响 [J]．中国实验方剂学杂志， 2015 ， 21 ( 23 )： 135 - 138 .

朱长良 . 大鼠蛛网膜下腔出血后脑血管痉挛后缝隙连接形态学检测 [D]．南昌：南昌大学， 2013 .

S Hoang ， J Liauw ， M Choi ， et al . Netrin-4 enhances angiogenesis and neurologic outcome after cerebral ischemia [J]. Cereb Blood Flow Metab ， 2008 ， 29 ( 2 )： 385 - 397 .

罗燕 . 针刺对脑缺血模型大鼠突触重建促进作用的研究 [D]．广州：广州中医药大学， 2009 .

杨丽，徐利满，朱晓丹，等．金芪降糖片对糖尿病引发的认知功能障碍的影响及机制 [J]．中国实验方剂学杂志， 2017 ， 23 ( 1 )： 146 - 152 .

白鑫宇，刘萍，杨帆，等．补阴益智汤配方颗粒对东莨菪碱所致小鼠学习记忆损伤的保护作用 [J]．中国实验方剂学杂志， 2017 ， 23 ( 12 )： 138 - 144 .

郭敏，李刚．突触可塑性相关蛋白的研究进展 [J]．神经药理学报， 2013 ， 3 ( 6 )： 57 - 64 .

S E Smith ， S A Figley ， D J Schreyer . Protein-energy malnutrition developing after global brain ischemia induces an atypical acute-phase response and hinders expression of GAP-43 [J]． PLoS One ， 2014 ， 9 ( 9 )： e107570 .

T WANG ， D R YU ， J HUANG ， et al . Multimodal rehabilitation program promotes motor function recovery of rats after ischemic stroke by upregulating expressions of GAP-43, SYN, HSP70, and C-MYC [J]． Stroke Cerebrovasc Dis ， 2018 ， 27 ： 2829 - 2839 .

孙晓彩，李力，张敏，等 . Wistar大鼠海马CA1区、CA3区和齿状回区的解剖分割 [J]．中国应用生理学杂志， 2012 ， 28 ( 2 )： 189 - 192 .

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Systematic Review and Sequential Analysis on Efficacy of Modified Buyang Huanwu Tang in Treatment of Chronic Heart Failure

Effect of Buyang Huanwu Tang on Axonal Regeneration of Rats After Ischemic Stroke Injury

Naoxintong Inhibits LPS Induced BV2 Cell Pyroptosis by NLRP3/Caspase-1 Signaling Pathway

Effect of Modified Buyang Huanwu Tang on Patients with Lumbar Disc Herniation After Percutaneous Foramen Endoscopy Based on Collateral Disease Theory

Clinical Efficacy of Modified Buyang Huanwu Tang Combined with Sanrentang in Treatment of Spleen and Kidney Deficiency and Damp-heat and Blood Stasis Syndrome in Early Diabetic Nephropathy

Related Author

Xiao-bo WU

Yu-qing TAN

Pan-pan TIAN

Heng-wen CHEN

Jun LI

Ai-ming YU

Xiang-li YAN

Hao-zhen ZHENG

Related Institution

Beijing University of Chinese Medicine

Guang'anmen Hospital， China Academy of Chinese Medical Sciences

School of Pharmaceutical Sciences， Guangzhou University of Chinese Medicine

Guizhou University of Traditional Chinese Medicine

Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰