Effect of Serum Containing Yanghetang on Apoptosis of MCF-7 Cells in Breast Cancer Through p38/STAT3 Signaling Pathway

Shuo YANG; Kang-le LI; Zhong-bo ZHU; Qian HUANG; Jia YAO; Yuan ZHI; Jian-wei DOU

doi:10.13422/j.cnki.syfjx.20192222

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Effect of Serum Containing Yanghetang on Apoptosis of MCF-7 Cells in Breast Cancer Through p38/STAT3 Signaling Pathway

Classic Prescriptions | 更新时间：2021-02-09

- Effect of Serum Containing Yanghetang on Apoptosis of MCF-7 Cells in Breast Cancer Through p38/STAT3 Signaling Pathway
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 26, Issue 5, Pages: 6-10(2020)
- 作者机构：
  
  1.甘肃中医药大学　基础医学院，兰州　 730000
  2.西安交通大学　药学院，西安　 710061
  3.西安市中医医院，西安　 710021
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20192222
  CLC： R22; R242; R2-031; R285.5
- Received：16 May 2019，
  
  Published Online：02 August 2019，
  
  Published：05 March 2020
- 稿件说明：
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Shuo YANG, Kang-le LI, Zhong-bo ZHU, et al. Effect of Serum Containing Yanghetang on Apoptosis of MCF-7 Cells in Breast Cancer Through p38/STAT3 Signaling Pathway[J]. Chinese journal of experimental traditional medical formulae, 2020, 26(5): 6-10.
DOI：

Shuo YANG, Kang-le LI, Zhong-bo ZHU, et al. Effect of Serum Containing Yanghetang on Apoptosis of MCF-7 Cells in Breast Cancer Through p38/STAT3 Signaling Pathway[J]. Chinese journal of experimental traditional medical formulae, 2020, 26(5): 6-10. DOI： 10.13422/j.cnki.syfjx.20192222.

摘要

目的：

以有丝分裂原激活蛋白激酶(p38)/信号传导与转录激活因子3(STAT3)信号通路为基础，探究阳和汤含药血清对乳腺癌MCF-7细胞凋亡的影响。

方法：

制备含生药1 g·mL

-1

的阳和汤药液，将SD大鼠随机分为空白组(蒸馏水)，阳和汤高、中、低剂量组(24，12，6 g·kg

-1

)，灌胃后制备阳和汤含药血清，用10%含药血清干预MCF-7细胞，通过细胞增殖及细胞毒性实验(CCK-8)检测阳和汤含药血清对MCF-7细胞增殖的影响；通过流式细胞术检测MCF-7细胞的凋亡情况；通过蛋白免疫印迹法(Western blot)检测p38和STAT3蛋白的表达情况；通过实时荧光定量聚合酶链式反应(Real-time PCR)检测B细胞淋巴瘤/白血病-xl(Bcl-xl)及生存素(Survivin) mRNA表达的情况。

结果：

CCK-8实验显示，与空白组比较，阳和汤含药血清抑制MCF-7细胞增殖，且呈时间与剂量依赖性。其中高剂量组抑制作用最为明显，不同时间抑制率分别达到38%，45%，54%(

0.01)；流式细胞术实验表明，与空白组比较，阳和汤含药血清中、高剂量组细胞凋亡率明显增加，且呈剂量依赖性，凋亡率分别达11.6%，16.5%(

0.05，

0.01)；Western blot表明，与空白组比较，阳和汤中、高剂量含药血清组p38，STAT3蛋白表达减少(

0.01)，且呈剂量依赖性；Real-time PCR实验表明，与空白组比较，阳和汤中、高剂量含药血清组Bcl-xl，Survivin mRNA表达减少(

0.05，

0.01)，且呈剂量依赖性。

结论：

阳和汤含药血清可以促进乳腺癌MCF-7细胞的凋亡，可能与p38/ STAT3信号通路有关。

Abstract

Objective:

To investigate the effect of serum containing Yanghetang (YHT) on the apoptosis of MCF-7 cells in breast cancer based on the mitogen-activated protein kinase (p38)/signal transduction and transcriptional activator 3 (STAT3) signal pathway.

Method:

YHT liquid with crude drug 1 g·mL

-1

was prepared. Female SD rats were randomly divided into control group (distilled water)

and high

medium and low-dose YHT groups (24

6 g·kg

-1

). YHT-medicated serum was prepared

and 10%medicated serum was used to intervene MCF-7 cells. Cell proliferation and cytotoxicity assay (CCK-8) was used to detect the effect of serum containing YHT on MCF-7 cell proliferation

apoptosis of MCF-7 cells was detected by flow cytometry protein expressions of p38 and STAT3 were detected by Western blot

Quantitative Real-time PCR (Real-time PCR) was used to detect the expressions of B-cell lymphoma/leukemia-xl(Bcl-xl) and Survivin mRNA.

Result:

CCK-8 assay showed that YHT serum inhibited the proliferation of MCF-7 cells in a time and dose-dependent manner compared with the blank group. The inhibitory effect was most obvious in the high-dose group

with the inhibition rates of 38%

45%and 54%at different time points (

0.01). Flow cytometry showed that

compared with the blank group

the apoptosis rate in the medium and high-dose groups increased significantly in a dose-dependent manner

with the apoptosis rates at 11.6%and 16.5%respectively (

0.05

0.01). Western blot analysis showed that

compared with the blank group

the expressions of p38 and STAT3 protein was decreased in high

medium-dose YHT groups (

0.01) in a dose-dependent manner. Compared with the blank group

the expressions of Bcl-xl and Survivin mRNA were decreased in high

medium-dose YHT groups (

0.05

0.01) in a dose-dependent manner.

Conclusion:

YHT serum can promote the apoptosis of MCF-7 cells in breast cancer

which may be related to the p38/ STAT3 signaling pathway.

关键词

Keywords

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