Effect of Modified Erchentang on VEGF, VEGFR2, IL-4, ET-1 in Chronic Obstructive Pulmonary Disease Rats

Wen-ying XIE; Jun-yue WANG; Yong-sheng BAO; Li-zhi SHANG; Jin-jing LI; Ke WU; Liang-zhi ZHANG; Liang LI; Xiao-yan WANG; Qian-hong SONG

doi:10.13422/j.cnki.syfjx.20192336

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Effect of Modified Erchentang on VEGF, VEGFR2, IL-4, ET-1 in Chronic Obstructive Pulmonary Disease Rats

Study on the Effect of Modified Erchentang on Chronic Obstructive Pulmonary Disease | 更新时间：2021-02-09

- Effect of Modified Erchentang on VEGF, VEGFR2, IL-4, ET-1 in Chronic Obstructive Pulmonary Disease Rats
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 23, Pages: 48-55(2019)
- 作者机构：
  
  河南中医药大学，郑州　 450046
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20192336
  CLC： R2-0; R285; R289
- Received：15 October 2018，
  
  Published Online：16 August 2019，
  
  Published：05 December 2019
- 稿件说明：
移动端阅览
Wen-ying XIE, Jun-yue WANG, Yong-sheng BAO, et al. Effect of Modified Erchentang on VEGF, VEGFR2, IL-4, ET-1 in Chronic Obstructive Pulmonary Disease Rats[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(23): 48-55.
DOI：

Wen-ying XIE, Jun-yue WANG, Yong-sheng BAO, et al. Effect of Modified Erchentang on VEGF, VEGFR2, IL-4, ET-1 in Chronic Obstructive Pulmonary Disease Rats[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(23): 48-55. DOI： 10.13422/j.cnki.syfjx.20192336.

摘要

目的：

观察二陈汤加味对慢性阻塞性肺疾病(COPD)大鼠血管内皮生长因子(VEGF)及其受体2(VEGFR2)，白细胞介素(IL)-4，内皮素-1(ET-1)及核转录因子-

B(NF-

B)的影响。

方法：

将50只SD大鼠随机分为5组，每组10只，组别为正常组、模型组、二陈汤加味低、中、高剂量组(10，20，40 g·kg

－1

·d

－1

)。以烟熏合并脂多糖(LPS)气管滴注的方法制备COPD大鼠模型。成功造模后，治疗组灌胃给药，正常组及模型组灌胃等体积蒸馏水。光镜观察大鼠肺血管的病理变化，并测定肺血管壁厚度。酶联免疫吸附测定(ELISA)检测大鼠血清、支气管肺泡灌洗液(BALF)和肺组织匀浆液中IL-4的含量，实时荧光定量PCR(Real-time PCR)检测ET-1 mRNA表达；免疫组化检测肺组织VEGF，ET-1及VEGFR2的表达。

结果：

与正常组比较，模型组大鼠血清，BALF及肺组织匀浆液中IL-4含量均显著降低(

0.01)；与模型组比较，二陈汤加味低、中、高剂量组IL-4含量均有程度不等的上升(

0.01)。与正常组比较，模型组肺组织ET-1 mRNA表达量显著升高(

0.01)；与模型组比较，二陈汤加味低、中、高剂量组肺组织ET-1 mRNA表达量均显著降低(

0.01)。与正常组比较，模型组大鼠肺组织中的VEGF，VEGFR2表达增多，ET-1蛋白表达显著上升(

0.01)，与模型组比较，二陈汤加味低、中、高剂量组VEGF，VEGFR2表达降低，ET-1蛋白表达显著降低(

0.01)。

结论：

二陈汤加味可能通过提升IL-4的含量，抑制VEGF，VEGFR2及ET-1的蛋白表达，从而减轻COPD大鼠肺组织炎症及肺血管重构的进程，减缓COPD及其并发症的进展。

Abstract

Objective:

To observe the effects of Erchen on vascular endothelial growth factor (VEGF) and its receptor R2 (VEGFR2)

interleukin (IL)-4 and endothelin-1 (ET-1) in rats with chronic obstructive pulmonary disease (COPD).

Method:

The 50 SD rats were randomly divided into 5 groups

10 rats in each group

which were normal group

model group

Erchentang low

medium and high dose group (10

40 g·kg

-1

·d

-1

). COPD rat model was established by smoking combined with lipopolysaccharide (LPS) intratracheal drip. After successful modeling

the treatment group was given intragastric administration

and the normal group and the model group were given intragastric distilled water of equal volume. The pathological changes of pulmonary vessels in rats were observed by light microscopy

and the thickness of pulmonary vascular wall was measured. The concentration of IL-4 in rat serum

bronchoalveolar lavage fluid (BALF) and lung homogenate was measured by enzyme-linked immunosorbent assay (ELISA). Real-time fluorescence quantitative polymerase chain reaction (Real-time PCR) was used to detect the expression of ET-1 and immunohistochemistry was used to detect the expression of VEGF

VEGFR2 and ET-1 in lung tissue.

Result:

Compared with normal group

the concentration of IL-4 in serum

BALF and lung homogenate of model group rats decreased significantly (

0.05). Compared with model group

the concentration of IL-4 in low

medium and high dose Erchentang group increased in varying degrees (

0.05). Compared with normal group

the expression of ET-1 in lung tissue of model group was significantly increased (

0.05) .C ompared with model group

the expression of ET-1 in lung tissue of low

medium and high dose Erchentang group was significantly decreased (

0.05). Compared with normal group

the expression of VEGF

VEGFR2

ET-1 protein in the lung tissue of model group increased (

0.05). Compared with model group

the expression of VEGF

VEGFR2 and ET-1 protein in the low

medium and high dose Erchentang group decreased (

0.05).

Conclusion:

Modified Erchentang can alleviate the process of pulmonary inflammation and pulmonary vascular remodeling in COPD rats

and slow down the progress of COPD and its complications by increasing the content of IL-4

inhibiting the expression of VEGF

VEGFR2

ET-1.

关键词

Keywords

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