Effect of Modified Erchentang on Expression of IL-19, IL-20R<sub>1</sub>, IL-20R<sub>2</sub> in Bronchioles of Rats with Chronic Obstructive Pulmonary Disease

Li-li XU; Li-zhi SHANG; Bo-yu WANG; Fan YANG; Yi-hang DU; Di ZHANG; Ping-ping HUANG; Xue FENG; Dong-jie ZHANG; Yi-Hui FENG; Wen-ying XIE

doi:10.13422/j.cnki.syfjx.20192338

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Effect of Modified Erchentang on Expression of IL-19, IL-20R₁, IL-20R₂ in Bronchioles of Rats with Chronic Obstructive Pulmonary Disease

Study on the Effect of Modified Erchentang on Chronic Obstructive Pulmonary Disease | 更新时间：2021-02-09

- Effect of Modified Erchentang on Expression of IL-19, IL-20R₁, IL-20R₂ in Bronchioles of Rats with Chronic Obstructive Pulmonary Disease
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 23, Pages: 41-47(2019)
- 作者机构：
  
  河南中医药大学，郑州　 450046
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20192338
  CLC： R2-0; R285; R289
- Received：06 June 2019，
  
  Published Online：16 August 2019，
  
  Published：05 December 2019
- 稿件说明：
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Li-li XU, Li-zhi SHANG, Bo-yu WANG, et al. Effect of Modified Erchentang on Expression of IL-19, IL-20R₁, IL-20R₂ in Bronchioles of Rats with Chronic Obstructive Pulmonary Disease[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(23): 41-47.
DOI：

Li-li XU, Li-zhi SHANG, Bo-yu WANG, et al. Effect of Modified Erchentang on Expression of IL-19, IL-20R₁, IL-20R₂ in Bronchioles of Rats with Chronic Obstructive Pulmonary Disease[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(23): 41-47. DOI： 10.13422/j.cnki.syfjx.20192338.

摘要

目的：

观察二陈汤加味对慢性阻塞性肺病(COPD)大鼠细支气管中白细胞介素-19(IL-19)，及其受体IL-20R

，IL-20R

表达的影响，探索二陈汤加味对COPD抗炎的机制。

方法：

用香烟烟雾联合脂多糖(LPS)制备COPD大鼠模型，将实验大鼠随机分为6组：正常组，模型组，二陈汤加味高、中、低剂量组，急支糖浆组。正常组与模型组灌生理盐水4 mL·d

－1

，二陈汤加味高、中、低剂量组(20，10，5 g·kg

－1

·d

－1

)，急支糖浆组(12 g·kg

－1

·d

－1

)，各组均连续灌胃14 d，2次/d。观察大鼠一般情况；评价大鼠肺功能；酶联免疫吸附测定(ELISA)检测大鼠血清中IL-10，IL-19和IL-20的含量；光镜观察大鼠细支气管组织病理变化；免疫组化检测大鼠细支气管组织中IL-20R

和IL-20R

的表达情况。

结果：

与正常组比较，模型组的最大呼吸流量(PEF)，1 s用力呼吸容积(FEV

)，用力肺活量(FVC)，FEV

/FVC的值均显著降低(

0.05)，血清中IL-19，IL-20的含量明显升高(

0.05)，IL-10含量明显降低(

0.05)，细支气管组织中IL-20R

，IL-20R

的表达明显升高(

0.05)。与模型组比较，急支糖浆组、二陈汤加味高、中、低剂量组的PEF，FEV

，FVC，FEV

/FVC均明显升高(

0.05)；血清中IL-19，IL-20的含量明显降低(

0.05)，IL-10含量明显升高(

0.05)；细支气管组织中IL-20R

，IL-20R

的表达明显降低(

0.05)。

结论：

二陈汤加味可以改善COPD大鼠的肺功能，保护细支气管的组织结构，其机制可能与二陈汤加味抑制大鼠细支气管中IL-19，IL-20及其受体IL-20R

，IL-20R

的表达有关。

Abstract

Objective:

To observe the effect of modified Erchentang on the expression of interleukin-19 (IL-19)

interleukin-20 (IL-20)and their receptor IL-20R

IL-20R

in bronchioles of rats with chronic obstructive pulmonary disease (COPD)

and to explore the molecular mechanism of modified Erchentang on anti-inflammatory of COPD.

Method:

The model of rat with COPD was established by cigarette smoke combined with lipopolysaccharide (LPS). The experimental rats were randomly divided into 6 groups: normal group

model group

modified Erchentang high

medium and low dose group

and Jizhitangjiang group. Normal group and model group fed with normal saline 4 mL·d

-1

modified Erchentang high

middle

low dosage group(20

5 g·kg

-1

·d

-1

). The dosage of Jizhitangjiang group was 12 g·kg

-1

·d

-1

all groups were given intragastric administration for 14 days

twice a day. To observe the general situation of rats.To evaluate the pulmonary function of rats. To detect the contents of IL-10

IL-19 and IL-20 in serum by enzyme-linked immunosorbent assay (ELISA). To observe the pathological changes of bronchiole tissue by light microscopy.To detect the expression of IL-20R

and IL-20R

in bronchiole tissue by immunohistochemistry.

Result:

Compared with normal group

peak expiratory flow(PEF)

forced expiratory volume in one second(FEV

)

forced vital capacity (FVC)

and FEV

/FVC in model group were significantly increased (

0.05)

the content of IL-19

IL-20 in serum significantly increased(

0.05)

the content of IL-10 in serum was significantly decreased (

0.05)

the expression of IL-20R

and in bronchioles tissue significantly increased (

0.05). Compared with model group

the PEF

FEV

FVC

FEV

/FVC of Jizhitangjiang group

modified Erchentang high

medium and low dosage group were significantly increased(

0.05). The content of IL-19

IL-20 in serum significantly decreased(

0.05)

the content of IL-10 in serum was significantly increased (

0.05)

the expression of IL-20R

and IL-20R

in bronchioles tissue was significantly decreased (

0.05).

Conclusion:

Modified Erchentang can improve the lung function and protect the tissue structure of bronchioles in COPD rats

which may be related to the inhibition of the expression of IL-19

IL-20 and their receptor IL-20R

IL-20R

in bronchioles of rats with modified Erchentang.

关键词

Keywords

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⁰

Effect of Modified Erchentang on Expression of IL-19, IL-20R1, IL-20R2 in Bronchioles of Rats with Chronic Obstructive Pulmonary Disease

DOI：10.13422/j.cnki.syfjx.20192338

摘要

Abstract

关键词

Keywords

references

Effect of Modified Erchentang on Expression of IL-19, IL-20R₁, IL-20R₂ in Bronchioles of Rats with Chronic Obstructive Pulmonary Disease