Isolation and Identification of Chemical Constituents from Rhizomes of <italic style="font-style: italic">Dendropanax proteus</italic>

Jian ZHOU; Yu-hua LI; Ying ZHOU; Yan-ni LYU; Hong-wei PENG; Ming-jin JIANG; Song TAO; Jin-hua WEN; Xiao-hua WEI

doi:10.13422/j.cnki.syfjx.20192349

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Isolation and Identification of Chemical Constituents from Rhizomes of Dendropanax proteus

Resource and Quality Evaluation | 更新时间：2021-02-09

- Isolation and Identification of Chemical Constituents from Rhizomes of Dendropanax proteus
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 23, Pages: 128-132(2019)
- 作者机构：
  
  南昌大学　第一附属医院，南昌　 330006
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20192349
  CLC： R22; R284; R282; O657; R931
- Received：25 June 2019，
  
  Published Online：08 August 2019，
  
  Published：05 December 2019
- 稿件说明：
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Jian ZHOU, Yu-hua LI, Ying ZHOU, et al. Isolation and Identification of Chemical Constituents from Rhizomes of Dendropanax proteus[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(23): 128-132.
DOI：

Jian ZHOU, Yu-hua LI, Ying ZHOU, et al. Isolation and Identification of Chemical Constituents from Rhizomes of Dendropanax proteus[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(23): 128-132. DOI： 10.13422/j.cnki.syfjx.20192349.

摘要

研究变叶树参95%乙醇提取物的化学成分及其活性。运用硅胶柱色谱、中压液相制备色谱、制备液相色谱等技术对变叶树参的95%乙醇提取物进行了系统的分离，并结合UV，ESI-MS，

H-NMR，

C-NMR等波谱学手段对所得到的化合物进行结构鉴定；采用Griess法检测一氧化氮(NO)浓度以评价各化合物的抗炎活性。从变叶树参的95%乙醇提取物中分离得到9个化合物，鉴定其分别为(－)-丁香脂素(

)，(＋)-(7

，8

)-1′，4-dihydroxy-3，3′，5′-trimethoxy-7′，8，9′-trinor-8，4′-oxyneoligna-7，9-diol(

)，赤式-愈创木基甘油-

-4′-松柏基醚(

)，苏式-愈创木基甘油-

-4′-松柏基醚(

)，松柏醇(

)，7-

-ethylguaiacylglycerol(

)，香草醛(

)，丁香醛(

)和excoecanol B(

)。化合物

和

可以抑制脂多糖(LPS)诱导的BV2细胞的NO释放，二者的半数抑制浓度(IC

)分别为5.85，7.29 μmol·L

－1

。化合物

1～6，8～9

均为首次从该植物中分离得到，化合物

和

具有一定的抗炎活性。

Abstract

The chemical constituents from 95% ethanol extract of

Dendropanax proteus

rhizomes and their anti-inflammatory activities were investigated. These compounds in 95% ethanol extract of

proteus

rhizomes were isolated and purified by silica gel column chromatography

medium-pressure liquid chromatography

preparative liquid chromatography

etc. Their structures were elucidated based on the spectral data and physicochemical properties. All the compounds were tested for their ability to inhibit lipopolysaccharide (LPS) induced nitric oxide production in the murine microglia BV2 cell line. Nine compounds were isolated from the ethyl acetate fraction of 95% ethanol extract of

proteus

rhizomes

and identified as (-)-syringaresinol (

)

(+ )-(7

)-1′

4-dihydroxy-3

3′

5′-trimethoxy-7′

9′-trinor-8

4′-oxyneoligna-7

9-diol (

)

erythro-guaiacylglycerol-

-4′-coniferyl ether (

)

threo-guaiacylglycerol-

-4′-coniferyl ether (

)

coniferyl alcohol (

)

-ethylguaiacylglycerol (

)

vanillin (

)

syringaldehyde (

)

and excoecanol B (

). Compounds

and

showed neuritis inhibitory activity against microglial inflammation factor

their half inhibitory concentrations (IC

) were 5.85

7.29 μmol·L

-1

respectively. Compounds

1-6

8-9

are isolated from this plant for the first time

compounds

and

exhibit the potent inhibitory activity.

关键词

Keywords

references

中国科学院《中国植物志》编辑委员会．中国植物志 [M]．北京：科学出版社， 1978 ： 58 - 61 ．

Chien S C , Tseng Y H , Hsu W N , et al . Anti-inflammatory and anti-oxidative activities of polyacetylene from Dendropanax dentiger [J]. Nat Prod Commun , 2014 , 9 ( 11 ): 1589 - 1590 .

Chung I M , SONG H K , Kim S J , et al . Anticomplement activity of polyacetylenes from leaves of Dendropanax morbifera Leveille . [J]. Phytother Res , 2011 , 25 ( 5 ): 784 - 786 .

任刚，罗仉平，黄慧莲，等．畲药半边风化学成分研究 [J]．中药材， 2012 ， 35 ( 1 )： 62 - 64 ．

LAI Y C , Lee S S . Chemical constituents from Dendropanax dentiger [J]. Nat Prod Commun , 2013 , 8 ( 3 ): 363 - 365 .

Park B Y , Min B S , Oh S R , et al . Isolation and anticomplement activity of compounds from Dendropanax morbifera [J]. J Ethnopharmacol , 2004 , 90 ( 2/3 ): 403 - 408 .

Richmond J D , Agius B R , Wright B S , et al . Essential oil compositions and cytotoxic activities of Dendropanax capillaris, Oreopanax nubigenus, and Schefflera rodrigueziana from Monteverde, Costa Rica [J]. Nat Prod Commun , 2009 , 4 ( 2 ): 271 - 274 .

Choo G S , Lim D P , Kim S M , et al . Anti-inflammatory effects of Dendropanax morbifera in lipopolysaccharide-stimulated RAW264.7 macrophages and in an animal model of atopic dermatitis [J]. Mol Med Rep , 2019 , 19 ( 3 ): 2087 - 2096 .

Youn J S , Kim Y J , Na H J , et al . Antioxidant activity and contents of leaf extracts obtained from Dendropanax morbifera LEV are dependent on the collecting season and extraction conditions [J]. Food Sci Biotechnol , 2018 , 28 ( 1 ): 201 - 207 .

Hyun T K , Kim M O , Lee H , et al . Evaluation of anti-oxidant and anti-cancer properties of Dendropanax morbifera Léveille [J]. Food Chem , 2013 , 141 ( 3 ): 1947 - 1955 .

SUN S , LI T , JIN L , et al . Dendropanax morbifera prevents cardiomyocyte hypertrophy by inhibiting the Sp1/GATA4 pathway [J]. Am J Chin Med , 2018 , 46 ( 5 ): 1021 - 1044 .

Choi J H , Kim D W , Park S E , et al . Anti-thrombotic effect of rutin isolated from Dendropanax morbifera Leveille . [J]. J Biosci Bioeng , 2015 , 120 ( 2 ): 181 - 186 .

Chung I M , Kim M Y , Park S D , et al . In vitro evaluation of the antiplasmodial activity of Dendropanax morbifera against chloroquine-sensitive strains of Plasmodium falciparum [J]. Phytother Res , 2009 , 23 ( 11 ): 1634 - 1637 .

国家中医药管理局《中华本草》编委会．中华本草 [M]．上海：上海科学技术出版社， 1999 ： 794 ．

YU H J , CHEN C C , Shieh D J . The constituents from the leaves of Magnolia coco [J]. J Chin Chem Soc , 1998 , 45 ( 6 ): 773 - 778 .

XIONG L , ZHU C , LI Y , et al . Lignans and neolignans from Sinocalamus affinis and their absolute configurations [J]. J Nat Prod , 2011 , 74 ( 5 ): 1188 - 1200 .

朱梅，熊亮，王亚男，等．慈竹茹中木脂素类化学成分的研究 [J]．中国中药杂志， 2012 ， 37 ( 13 )： 1968 - 1972 ．

HAN H Y , WANG X H , WANG N L , et al . Lignans isolated from Campylotropis hirtella (Franch.) Schindl. decreased prostate specific antigen and androgen receptor expression in LNCaP cells [J]. J Agric Food Chem , 2008 , 56 ( 16 ): 6928 - 6935 .

仲欢欢，方诗琦，陈亚军，等．野马追化学成分及其抗炎活性 [J]．中成药， 2017 ， 39 ( 2 )： 330 - 333 ．

YANG X W , ZHAO P J , MA Y L , et al . Mixed lignan-neolignans from Tarenna attenuate [J]. J Nat Prod , 2007 , 70 ( 4 ): 521 - 525 .

吕凌岳，张岱州，王芳，等．过山蕨乙醇提取物化学成分的分离鉴定 [J]．中国实验方剂学杂志， 2019 ， 25 ( 14 )： 168 - 172 ．

朱伶俐，艾志福，徐丽，等．桂枝化学成分的分离鉴定 [J]．中国实验方剂学杂志， 2019 ， 25 ( 14 )： 173 - 178 ．

HUANG S Z , LUO H R , MA Q Y , et al . Chemical constituents from the stems of Excoecaria acertiflia [J]. Chem Biodivers , 2014 , 11 ( 9 ): 1406 - 1416 .

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Related Institution

Puyang Center for Food and Drug Inspection and Test

Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College

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Isolation and Identification of Chemical Constituents from Rhizomes of Dendropanax proteus

DOI：10.13422/j.cnki.syfjx.20192349

摘要

Abstract

关键词

Keywords

references