Application of DESI-MSI in Quality Control of Banxia Xiexintang

Bo SUN; Yi-fan ZHAO; Guang-wei ZHU; Dong ZHANG; Yuan-zhang QU; A-qian CHANG; Teng-teng XU; Cui ZHANG

doi:10.13422/j.cnki.syfjx.20192350

您当前的位置：

首页 >

文章列表页 >

Application of DESI-MSI in Quality Control of Banxia Xiexintang

Pharmacy Fundamentals | 更新时间：2021-02-09

- Application of DESI-MSI in Quality Control of Banxia Xiexintang
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 26, Issue 7, Pages: 117-128(2020)
- 作者机构：
  
  1.哈尔滨商业大学，哈尔滨　 150076
  2.中国中医科学院　中药研究所，北京　 100700
  3.山东中医药大学，济南　 250355
  4.北京中医药大学，北京　 100102
  5.江西青峰药业有限公司　创新天然药物与中药注射剂国家重点实验室，江西赣州　 341000
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20192350
  CLC： R22; O657; R28; C37; R914
- Received：21 June 2019，
  
  Published Online：13 August 2019，
  
  Published：05 April 2020
- 稿件说明：
移动端阅览
Bo SUN, Yi-fan ZHAO, Guang-wei ZHU, et al. Application of DESI-MSI in Quality Control of Banxia Xiexintang[J]. Chinese journal of experimental traditional medical formulae, 2020, 26(7): 117-128.
DOI：

Bo SUN, Yi-fan ZHAO, Guang-wei ZHU, et al. Application of DESI-MSI in Quality Control of Banxia Xiexintang[J]. Chinese journal of experimental traditional medical formulae, 2020, 26(7): 117-128. DOI： 10.13422/j.cnki.syfjx.20192350.

摘要

目的：

制备不同产地15批次半夏泻心汤物质基准及对应实物，分别应用超高效液相色谱-二极管阵列检测器检测法(UHPLC-DAD)与解吸电喷雾电离-敞开式离子化质谱成像技术(DESI-MSI技术)对不同产地15批次半夏泻心汤对应实物进行分析，考察DESI-MSI在经典名方质量控制中的优势。

方法：

以半夏泻心汤为研究模型，采用UHPLC-DAD建立半夏泻心汤物质基准及对应实物指纹图谱，并对指标成分含量、出膏率进行考察。同时，以半夏泻心汤对应实物冻干粉为研究载体，将其复溶后以5 μL定量毛细管点样于定性滤纸，并固定于载玻片制成样本，以甲醇-甲酸(1 000∶1)为喷雾溶剂，流量3 μL·min

-1

，扫描范围100～1 200 Da，空间分辨率300 μm，喷雾电压3 kV，采样锥电压±40 V，喷雾器(N

)压力0.5 MPa，喷雾器入射角60度，收集角10度，离子源温度120 ℃，正、负离子全扫描样本。

结果：

DESI-MSI能检测到指标成分甘草苷、黄芩苷、汉黄芩苷，及共有峰芹糖基甘草苷、小檗碱、甘草酸等，并对其半定量分析，分析结果与UHPLC-DAD基本一致。同时，DESI-MSI还能检测到来自甘草、黄连、黄芩、大枣和人参的甘草皂苷G

，巴马汀，黄连碱，芦丁，人参皂苷Rg

等16种成分，并对其相对含量进行直观呈现，定性分析能力远强于UHPLC-DAD。

结论：

DESI-MSI技术无需进行复杂的样品处理过程，且灵敏度高、分析能力强，可以在无对照品情况下进行复杂样品的定性和相对含量分析，可作为经典名方物质基准、对应实物及配方颗粒样品的质控手段。

Abstract

Objective:

To prepare 15 batches of Banxia Xiexintang substance benchmark and lyophilized powder from different places

and the lyophilized powder was analyzed by ultra-high performance liquid chromatography with diode array detection (UHPLC-DAD) and desorption electrospray ionization-mass spectrometry imaging (DESI-MSI) in order to investigate the advantages of DESI-MSI in quality control of famous classical formulas.

Method:

Taking Banxia Xiexintang as the research model

fingerprints of the substance benchmark and lyophilized powder were established by UHPLC-DAD

and the content of index components and the yield of dry extract were also investigated. Meanwhile

as the research carrier

the lyophilized powder corresponding to Banxia Xiexintang was dissolved in methanol and dotted on qualitative filter paper with 5 μL quantitative capillary

and fixed it on the slide to make samples. The samples were analyzed on a DESI-MSI system in positive and negative ion mode with methanol-formic acid (1 000∶1

flow rate of 3 μL·min

-1

) as spray solvent

as spray gas (pressure of 0.5 MPa). The scanning range was 100-1 200 Da

the spatial resolution was 300 μm

the spray voltage was 3 kV

the sampling cone voltage was ±40 V

incidence angle of sprayer was 60 degree

its collection angle was 10 degree

the ion source temperature was 120 ℃.

Result:

DESI-MSI could not only detect the index components of liquiritin

baicalin and wogonoside

as well as the common peaks of liquiritin apioside

berberine and glycyrrhizic acid

but also analyzed them semi-quantitatively

the analysis results were basically consistent with UHPLC-DAD. At the same time

DESI-MSI could detect 16 other components from Glycyrrhizae Radix et Rhizoma

Coptidis Rhizoma

Scutellariae Radix

Jujubae Fructus and Ginseng Radix et Rhizoma

such as licoricesaponin G

palmatine

coptisine

rutin and ginsenoside Rg

and present their relative content visually. The qualitative analysis ability of DESI-MSI was much better than UHPLC-DAD.

Conclusion:

DESI-MSI can be used as the quality control method for substance benchmark and lyophilized powder and dispensing granules of famous classical formulas with advantages of high sensitivity

strong analytical ability

no complex sample processing

qualitative and relative content analysis of complex samples without reference substance.

关键词

Keywords

references

张嘉鑫，顾然，郭宇，等．半夏泻心汤之方药考 [J]．长春中医药大学学报， 2018 ， 34 ( 1 ): 182 - 185 .

王惠英，曹圣荣．半夏泻心汤方证探微 [J]．实用中医内科杂志， 2017 ， 31 ( 9 ): 76 - 79 .

童荣生，李晋奇，彭成，等．半夏泻心汤及其苦降药组HPLC指纹图谱的比较研究 [J]．中华中医药学刊， 2011 ， 29 ( 7 ): 1578 - 1581 .

王瑞，王志刚．半夏泻心汤治疗痞证验案举隅 [J]．中国民族民间医药， 2018 ， 27 ( 16 ): 74 - 75 .

邓哲，刘德文，杜杰，等．经典名方研发建议的梳理和探讨 [J]．中国实验方剂学杂志， 2019 ， 25 ( 17 ): 181 - 186 .

唐晓章，林美斯，周菲，等．经典名方芍药甘草汤的物质基准量值传递分析 [J]．中国实验方剂学杂志， 2019 ， 25 ( 14 ): 62 - 69 .

冯红，潘桂湘．高分辨质谱在中药化学成分分析中的应用 [J]．辽宁中医药大学学报， 2012 ， 14 ( 8 ): 40 - 42 .

刘念，刘锋，许彬，等．生物组织质谱成像方法的建立及其在微波辐射后大鼠海马组织的蛋白组分析中的应用 [J]．分析化学， 2008 ， 36 ( 4 ): 421 - 425 .

C P WU , D R IFA , N E MANICKE ， et al . Rapid，direct analysis of cholesterol by charge labeling in reactive desorption electrospray ionization [J]． Anal Chem ， 2009 ， 81 ( 18 ): 7618 - 7624 .

A L DILL , L S EBERLIN , C ZHENG ， et al . Multivariate statistical differentiation of renal cell carcinomas based on lipidomic analysis by ambient ionization imaging mass spectrometry [J]． Anal Bioanal Chem ， 2010 ， 398 ( 7/8 ): 2969 - 2978 .

邓远雄，李晓宇．体内药物分析 [M]．长沙：中南大学出版社， 2016 ： 188 - 192 .

J TILLNER , V WU , E A JONES ， et al . Faster，more reproducible DESI-MS for biological tissue imaging [J]． J Am Soc Mass Spectrom ， 2017 ， 28 ( 10 ): 2090 - 2098 .

Z TAKÁTS , J M WISEMAN , R G COOKS ． Ambient mass spectrometry using desorption electrospray ionization (DESI)：instrumentation，mechanisms and applications in forensics，chemistry，and biology [J]． J Mass Spectrom ， 2005 ， 40 ( 10 ): 1261 - 1275 .

N ABBASSI-GHADI , E A JONES , K A VESELKOV ， et al . Repeatability and reproducibility of desorption electrospray ionization-mass spectrometry (DESI-MS) for the imaging analysis of human cancer tissue：a gateway for clinical applications [J]． Anal Methods ， 2015 ， 7 ( 1 ): 71 - 80 .

Z MIAO , H CHEN ． Direct analysis of liquid samples by desorption electrospray ionization-mass spectrometry (DESI-MS) [J]． J Am Soc Mass Spectrom ， 2009 ， 20 ( 1 ): 10 - 19 .

王中华，何秉淑，孙成龙，等．基于空气动力辅助离子化-超高分辨质谱成像技术的大鼠肾脏组织中多种类代谢物的分布研究 [J]．分析化学， 2018 ， 46 ( 3 ): 406 - 412 .

李冀．方剂学 [M]. 2版．北京：中国中医药出版社， 2016 ： 63 - 65 .

闫利利，史家文，王金芳，等．基于UPLC/Q-TOF-MS E 方法分析半夏泻心汤的化学成分 [J]．药学学报， 2013 ， 48 ( 4 ): 526 - 531 .

惠永正．中药天然产物大全 [M]．上海：上海科学技术出版社， 2011 ： 1818，3204，7783 .

M YE , S H LIU , Z L JIANG ， et al . Liquid chromatography/mass spectrometry analysis of PHY906，a Chinese medicine formulation for cancer therapy [J]． Rapid Commun Mass Spectrom ， 2007 ， 21 ( 22 ): 3593 - 3607 .

吴茵，白万军，魏欣．基于UPLC-Q-TOF-MS技术分析木蝴蝶中化学成分 [J]．中国实验方剂学杂志， 2019 ， 25 ( 2 ): 196 - 200 .

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Establishment of Specific Chromatogram and Thin-layer Identification Method for Kaixinsan

Establishment of Specific Chromatogram and TLC Identification for Qingxin Lianziyin

Establishment of Anti-inflammatory Activity Evaluation Method of Qingjin Huatantang Based on Phosphodiesterase Inhibitory Activity

Analysis of Specific Chromatogram of Wenjingtang Reference Sample

Quality Value Transmitting of Substance Benchmark of Xiebaisan

Related Author

GAO Wenya

FENG Min

GAO Chang

ZHAO Haiyu

ZHOU Yanyan

WANG Hongjie

YU Ruipeng

ZHAO Yipeng

Related Institution

Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences

Sunflower Pharmaceutical Group Co. Ltd.

Beijing Institute for Drug Control

Sunflower Pharmaceutical Group（Jizhou） Co. Ltd.

Institute of Chinese Materia Media， China Academy of Chinese Medical Sciences

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰