Effect of Icariin on Damaged Neurons Based on Endoplasmic Reticulum Stress

Cheng-jie WU; Yong MA; Yang GUO; Lei WANG; Peng-cheng TU; Ya-lan PAN; Su-yang ZHENG; Gui-cheng HUANG

doi:10.13422/j.cnki.syfjx.20192402

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Effect of Icariin on Damaged Neurons Based on Endoplasmic Reticulum Stress

Pharmacology | 更新时间：2021-04-16

- Effect of Icariin on Damaged Neurons Based on Endoplasmic Reticulum Stress
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 26, Issue 2, Pages: 59-65(2020)
- 作者机构：
  
  1.南京中医药大学　骨伤研究所，南京　 210023
  2.南京中医药大学　附属医院，南京　 210029
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20192402
  CLC： R2-0; R22; R285.5; R289
- Received：20 May 2019，
  
  Published Online：04 September 2019，
  
  Published：20 January 2020
- 稿件说明：
移动端阅览
Cheng-jie WU, Yong MA, Yang GUO, et al. Effect of Icariin on Damaged Neurons Based on Endoplasmic Reticulum Stress[J]. Chinese journal of experimental traditional medical formulae, 2020, 26(2): 59-65.
DOI：

Cheng-jie WU, Yong MA, Yang GUO, et al. Effect of Icariin on Damaged Neurons Based on Endoplasmic Reticulum Stress[J]. Chinese journal of experimental traditional medical formulae, 2020, 26(2): 59-65. DOI： 10.13422/j.cnki.syfjx.20192402.

摘要

目的：

从内质网应激的角度观察淫羊藿苷对受损神经元的影响，并探究其修复受损神经元的部分机制。

方法：

采用神经生长因子(NGF)将PC12细胞诱导分化为神经元，并使用流式细胞术测定微管相关蛋白2(MAP2)和神经元特异性烯醇化酶(NSE)表达阳性率进行鉴定。实验分为4组，空白组为PC12诱导分化的神经元细胞；溶剂组为PC12诱导分化的神经元＋0.1%二甲基亚砜(DMSO)；毒胡萝卜素组为PC12诱导分化的神经元＋2 μmol·L

-1

毒胡萝卜素；淫羊藿苷组为PC12诱导分化的神经元＋2 μmol·L

-1

毒胡萝卜素＋0.1 μmol·L

-1

淫羊藿苷，使用细胞增殖活性检测试剂盒(CCK-8)法检测细胞的增殖，流式细胞术检测细胞的凋亡，蛋白免疫印迹法(Western blot)检测CCAAT/增强子结合蛋白同源蛋白(CHOP)，葡萄糖调节蛋白78(Grp78)的蛋白表达，实时荧光定量聚合酶链式反应(Real-time PCR)检测CHOP，Grp78 mRNA表达。

结果：

与溶剂组比较，毒胡萝卜素组可抑制经NGF诱导的神经元样PC12细胞的增殖活性，可促使细胞凋亡，并能够上调CHOP，Grp78的表达(

0.05，

0.01)；与毒胡萝卜素组比较，淫羊藿苷组可缓解毒胡萝卜素对神经元增殖活性的抑制，减少神经元凋亡，并能够下调CHOP，Grp78的表达(

0.05，

0.01)。

结论：

淫羊藿苷可通过下调CHOP和Grp78的表达抑制内质网应激，促进受损神经元的修复。

Abstract

Objective:

To observe the effect of icariin on damaged neurons from the perspective of endoplasmic reticulum stress

in order to explore some mechanisms for repairing damaged neurons.

Method:

PC12 cells were induced by nerve growth factor (NGF) to differentiate into neurons

and the positive rate of microtubule associated protein-2 (MAP2) and neuron-specific enolase (NSE) expressions was determined by flow cytometry. The experiment was divided into 4 groups

blank control group: PC12 induced differentiation into neuronal cells

solvent control group: PC12 induced differentiation into neurons+ 0.1% dimethyl sulfoxide (DMSO)

thapsigargin group: PC12 induced differentiation into nerves Yuan+ 2 μmol·L

-1

thapsigargin

and icariin group: PC12 induced differentiation into neurons+ 2 μmol·L

-1

thapsigargin+ 0.1 μmol·L

-1

icariin. The proliferation of the cells was detected by using cell counting kit-8(CCK-8) method

the apoptosis of the cells was detected by flow cytometry

the protein expressions of CCAAT/enhace-binding protein homologous protein(CHOP) and glucoseregulated protein 78(Grp78) were detected by Western blot

and the mRNA expressions of CHOP and Grp78 were detected by real-time quantitative PCR (Real-time PCR).

Result:

Compared with the solvent control group

the thapsigargin group inhibited the proliferation of neuron-like PC12 cells induced by NGF

promoted apoptosis

and up-regulated the expressions of CHOP and Grp78 (

0.05

0.01). Compared with the thapsigargin group

the icariin group can alleviate the inhibition of neurotrophic activity by thapsigargin

reduce neuronal apoptosis

and down-regulate the expressions of CHOP and Grp78 (

0.05

0.01).

Conclusion:

Icariin can inhibit endoplasmic reticulum stress by down-regulating the expressions of CHOP and Grp78 and promote the repair of damaged neurons.

关键词

Keywords

references

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