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Identification of
Citrus medica var.sarcodactylis Infected with Citrus Huanglongbing- Chinese Journal of Experimental Traditional Medical Formulae Vol. 26, Issue 5, Pages: 117-122(2020)
- 作者机构：
广东药科大学 中药学院，国家中医药管理局 岭南药材生产与开发重点研究室，国家中药材产业技术体系 广州综合试验站，广东省南药规范化种植与综合开发工程技术研究中心，广州 510006
- 作者简介：
- 基金信息：
DOI：10.13422/j.cnki.syfjx.20192413
CLC： R289; R284.1; R22; R2-031Received：22 February 2019，
Published Online：04 September 2019，
Published：05 March 2020
- 稿件说明：
移动端阅览
Meng YUAN, Li-ming PAN, Liang-yun ZHOU, et al. Identification of
Citrus medica var.sarcodactylis Infected with Citrus Huanglongbing[J]. Chinese journal of experimental traditional medical formulae, 2020, 26(5): 117-122.Meng YUAN, Li-ming PAN, Liang-yun ZHOU, et al. Identification of
Citrus medica var.sarcodactylis Infected with Citrus Huanglongbing[J]. Chinese journal of experimental traditional medical formulae, 2020, 26(5): 117-122. DOI： 10.13422/j.cnki.syfjx.20192413.
摘要
目的：
2
该文从多方面进行分析、比较，及时、准确地鉴别广佛手黄龙病，以便于及时确定病害、防控病情。
方法：
2
通过性状分析，逆转录-聚合酶链式反应(RT-PCR)，酶切反应及实时荧光定量PCR(Real-time PCR)检测多种手段相结合，对广佛手黄龙病进行鉴定。
结果：
2
从性状上来看，感病广佛手有典型的叶片斑驳型黄化、果相对较小，甚至畸形的特征，但并没有发现“红鼻子果”现象，这些均可以作为田间初步判别广佛手黄龙病的依据；RT-PCR检测及酶切反应结果表明，引物为OI1/OI2c时，广佛手病株有特异性条带1 160 bp，且能被Xba I酶切成520 bp和640 bp，这与其他被黄龙病侵染的柑橘属植物的检测结果一致；Real-time PCR检测结果显示，被黄龙病侵染的广佛手叶片有扩增曲线和熔解曲线，熔链温度为82 ℃，且
C
t
值在24.6～28.2，而广佛手正常株没有扩增现象出现。
结论：
2
性状分析可以在田间初步判别黄龙病，但是具有一定的主观性，RT-PCR及Real-time PCR检测能进一步确定广佛手黄龙病，且qPCR检测更加灵敏，还可以定量。通过性状分析以及分子鉴定的结合，能够更加及时、准确地确定广佛手黄龙病。
Abstract
Objective:
2
To analyze and compare different samples in many aspects to identify
Citrus medica
var.
sarcodactylis
infected with Huanglongbing(HLB) timely and accurately
in order to prevent and control the disease in time.
Method:
2
HLB was identified through character analysis
reverse transcription-polymerase chain reaction(RT-PCR)
enzyme digestion reaction and Real-time PCR.
Result:
2
In terms of characters
there were typically variegated yellow leaves and relatively small fruit
even with deformity but without " red nose fruit" among
C
.
medica
var.
sarcodactylis
infected with HLB. All of these can be used as the basis for the preliminary identification of HLB in the fields. According to the RT-PCR test results and enzyme digestion reaction
when the primer was OI1/OI2c
there was specific band of 1 160 bp
which could be cut into 520 bp and 640 bp by Xba I enzyme. These results were consistent with the characters of other citrus plants infected with HLB. According to the Real-time PCR detection results
C
.
medica
var.
sarcodactylis
infected with HLB had amplification curves and dissolved peaks
with the melting temperature was 82 ℃ and
C
t
between 24.6 to 28.2
while the normal plants were not amplified.
Conclusion:
2
Character analysis can be used to roughly distinguish HLB in the fields
but with a certain subjectivity. RT-PCR or Real-time PCR can be used to identify
C
.
medica
var.
sarcodactylis
infected with HLB in a timely and accurate manner
and qPCR detection is more sensitive and quantitative. Through the combination of character analysis and molecular identification
C
.
medica
var.
sarcodactylis
infected with HLB can be determined more timely and accurately.
关键词
Keywords
references
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