Study on Frosting Quality Markers of Mori Folium Based on Component Characteristic

Yong-sheng QU; Liang WANG; Wei GUO

doi:10.13422/j.cnki.syfjx.20192418

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Study on Frosting Quality Markers of Mori Folium Based on Component Characteristic

Resource and Quality Evaluation | 更新时间：2021-02-09

- Study on Frosting Quality Markers of Mori Folium Based on Component Characteristic
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 23, Pages: 145-150(2019)
- 作者机构：
  
  山东省中医药研究院，济南　 250014
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20192418
  CLC： R284.2; R289; R22; R2-031
- Received：10 June 2019，
  
  Published Online：17 September 2019，
  
  Published：05 December 2019
- 稿件说明：
移动端阅览
Yong-sheng QU, Liang WANG, Wei GUO. Study on Frosting Quality Markers of Mori Folium Based on Component Characteristic[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(23): 145-150.
DOI：

Yong-sheng QU, Liang WANG, Wei GUO. Study on Frosting Quality Markers of Mori Folium Based on Component Characteristic[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(23): 145-150. DOI： 10.13422/j.cnki.syfjx.20192418.

摘要

目的：

分析识别桑叶经霜质量标志物(Q-marker)，对桑叶是否经霜进行鉴别。

方法：

建立桑叶经霜前后HPLC-DAD-MS

指纹图谱，标记共有峰，采用正交偏最小二乘判别分析(OPLS-DA)提取桑叶经霜后的特征成分，以含量变化较大的成分作为标志成分，较为稳定的成分作为内参成分，以标志成分与内参成分峰面积的相对比值为桑叶经霜成分特征，实现对桑叶经霜的鉴别，并通过MS

和对照品进行定性研究。

结果：

建立了桑叶经霜前后的指纹图谱，共标记了33个共有峰，通过OPLS-DA分析，峰1，23，14确定为标志成分，峰12确定为内参成分，通过MS

和对照品分别鉴定为枸橼酸衍生物，桑皮苷F，色氨酸和新绿原酸。桑叶经霜前后枸橼酸衍生物，桑皮苷F，色氨酸与新绿原酸峰面积的比值分别为0.15±0.054，1.0±0.48；0.14±0.073，0.98±0.48；0.13±0.088，0.89±0.49。

结论：

建立的桑叶指纹图谱中，枸橼酸衍生物，桑皮苷F，色氨酸与新绿原酸的峰面积比值作为桑叶经霜的成分特征具有专属性，可作为桑叶经霜Q-marker对桑叶是否经霜进行鉴别。该研究扩展了中药Q-marker专属性的内涵，可为桑叶的质量评价及成药性提供实验数据，并为类似研究提供参考。

Abstract

Objective:

To screen the frosting qualitymarkers (Q-marker) of Mori Foliumand identify Mori Folium after frost.

Method:

The HPLC-DAD-MS

fingerprints of Mori Folium before and after frost were established

the common peaks were markedand the characteristic components were selected by orthogonal partial least squares discrimination analysis (OPLS-DA). The components with a large content change were used as marker components

and the relatively stable component was used as an internal reference component. The ratio of the marker components to the peak area of the internal reference component was used as component characteristic to identify the frost of Mori Folium

andqualitative study of frosting markerswere performed by MS

and authentic standards.

Result:

The fingerprints were established and 33 common peaks were marked. Through OPLS-DA

peaks 1

14 were determined as marker components

peak 12 was determined as internal reference component. The frosting quality markers of Mori Folium were characterized as citric acid derivatives

saponin F

tryptophan and neochlorogenic acid by MS

and standards. The ratios of the peak areas of citric acid derivatives

saponin F

tryptophan and neochlorogenic acid before and after frost were 0.15±0.054

1.0±0.48; 0.14±0.073

0.98±0.48

0.13±0.088

0.89±0.49

respectively.

Conclusion:

In the fingerprints established in this study

the peak area ratios of citric acid derivatives

saponin F

tryptophan to new chlorogenic acid had specificity

which can be used as frosting quality markers for identification of Mori Folium. This study expanded the connotation of the Q-marker specificity of traditional Chinese medicine. The results can provide experimental data for the quality evaluation and drug-forming properties of Mori Folium

and provide reference for similar research.

关键词

Keywords

references

国家药典委员会．中华人民共和国药典．一部 [M]．北京：中国医药科技出版社， 2015 ： 297 - 298 ．

孙星衍，孙冯翼．神农本草经 [M]．太原：山西科学技术出版社， 1991 ．

李时珍．本草纲目 [M]．北京：华夏出版社， 2008 ．

陶弘景．本草经集注 [M]．上海：群联出版社， 1955 ．

寥希雍．神农本草经疏 [M]．北京：中医古籍出版社， 2002 ．

Yen G C , WU S C , Duh P D . Extraction and identification of antioxidant components from the leaves of mulberry (Morus alba L.) [J]. J Agric Food Chem , 1996 , 44 ( 7 ): 1687 - 1690 .

Yadav A V , Kawale L A , Nade V S . Effect of Morus alba L. (mulberry) leaves on anxiety in mice [J]. Indian J Pharmacol , 2008 , 40 ( 1 ): 32 - 36 .

季涛，宿树兰，郭盛，等．基于α-葡萄糖苷酶抑制活性评价桑叶多组分药效相互作用研究 [J]．中国中药杂志， 2016 ， 41 ( 11 )： 1999 - 2006 ．

刘颖慧，牟新，周迪夷，等．基于JNK信号通路探讨桑叶有效成分改善胰岛素抵抗的机制研究 [J]．中国中药杂志， 2019 ， 44 ( 5 )： 1019 - 1025 ．

王婷婷，马天宇，李琪，等．桑叶化学成分及生物活性研究进展 [J]．食品与药品， 2018 ， 20 ( 5 )： 390 - 393 ．

白华．《神农本草经》桑叶考证 [J]．内蒙古中医药， 2016 ， 35 ( 1 )： 102 - 103 ．

苏顷．本草图经 [M]．北京：人民卫生出版社， 2011 ．

黄宫绣．本草求真 [M]．北京：人民卫生出版社， 1987 ．

陈嘉谟．本草蒙筌 [M]．北京：人民卫生出版社， 1988 ．

游元元，万德光．川产桑叶“经霜为上”合理性的LC-MS法验证 [J]．时珍国医国药， 2011 ， 22 ( 11 )： 2596 - 2598 ．

张魏琬麒，欧阳臻，赵明，等．桑叶经霜前后次生代谢产物表达差异分析 [J]．食品科学， 2015 ， 36 ( 8 )： 109 - 114 ．

俞燕芳，黄金枝，王军文，等．霜后桑叶总酚、黄酮和总糖含量变化研究 [J]．蚕桑茶叶通讯， 2017 ( 6 )： 1 - 4 ．

张铁军，王杰，陈常青，等．基于中药属性和作用特点的中药质量标志物研究与质量评价路径 [J]．中草药， 2017 ， 48 ( 6 )： 1051 - 1060 ．

杨岩涛，李森，刘金玲，等．中药质量标志物与“网通虹势”代谢规律 [J]．中国中药杂志， 2017 ， 42 ( 12 )： 2420 - 2424 ．

王亮，窦立雯，郭威，等．基于中药传统用法的毒性Q-Marker发现：以吴茱萸为例 [J]．中草药， 2017 ， 48 ( 6 )： 1159 - 1166 ．

Spínola V , Pinto J , Castilho P C , et al . Identification and quantification of phenolic compounds of selected fruits from Madeira island by HPLC-DAD-ESI-MSn and screening for their antioxidant activity [J]. Food Chem , 2015 , 173 : 14 - 30 .

Lee S H , Choi S Y , Kim H , et al . Mulberroside F isolated from the leaves of Morus alba inhibits melanin biosynthesis [J]. Biol Pharm Bull , 2002 , 25 ( 8 ): 1045 - 1048 .

田欢，翟俊乐，李孟秋，等． LC-MS/MS法测定小鼠脑组织中色氨酸及其3种代谢物 [J]．食品工业科技， 2016 ， 37 ( 1 )： 315-319，330 ．

梅辉．基于LC-MS/MS法的色氨酸合成代谢组学分析平台的构建 [D]．无锡：江南大学， 2010 ．

张阿琴，李迩娜，张仓，等． LC-MS分析消癌平注射液中有机酸类成分 [J]．中国实验方剂学杂志， 2012 ， 18 ( 4 )： 88 - 90 ．

刘昌孝，陈士林，肖小河，等．中药质量标志物(Q-Marker)：中药产品质量控制的新概念 [J]．中草药， 2016 ， 47 ( 9 )： 1443 - 1457 ．

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