Analysis of Chemical Composition in Sancao Baogan Decoction Based on UPLC-ESI-HRMS<sup>n</sup>

Xiang-yi LIU; Rui ZENG; Chang-chuan YANG; Jing-lan PENG; Heng-xiu YAN

doi:10.13422/j.cnki.syfjx.20192448

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Analysis of Chemical Composition in Sancao Baogan Decoction Based on UPLC-ESI-HRMSⁿ

Pharmacy Fundamentals | 更新时间：2021-02-09

- Analysis of Chemical Composition in Sancao Baogan Decoction Based on UPLC-ESI-HRMSⁿ
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 26, Issue 7, Pages: 141-147(2020)
- 作者机构：
  
  西南民族大学　生命科学与技术学院，药学院，成都　 610041
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20192448
  CLC： R22; R28; C37; O657; R91
- Received：09 July 2019，
  
  Published Online：09 September 2019，
  
  Published：05 April 2020
- 稿件说明：
移动端阅览
Xiang-yi LIU, Rui ZENG, Chang-chuan YANG, et al. Analysis of Chemical Composition in Sancao Baogan Decoction Based on UPLC-ESI-HRMSⁿ[J]. Chinese journal of experimental traditional medical formulae, 2020, 26(7): 141-147.
DOI：

Xiang-yi LIU, Rui ZENG, Chang-chuan YANG, et al. Analysis of Chemical Composition in Sancao Baogan Decoction Based on UPLC-ESI-HRMSⁿ[J]. Chinese journal of experimental traditional medical formulae, 2020, 26(7): 141-147. DOI： 10.13422/j.cnki.syfjx.20192448.

摘要

目的：

利用UPLC-ESI-HRMS

技术对三草保肝汤中化学成分进行分析。

方法：

采用ACQUITY UPLC BEH C

色谱柱(2.1 mm×100 mm，1.7 μm)，以甲醇(A)-0.1%甲酸水溶液(B)为流动相进行梯度洗脱(0～2 min，5%A；2～20 min，5%～12%A；20～35 min，12%～40%A；35～38 min，40%A；38～48 min，40%～80%A；48～50 min，80%A)，流速0.3 mL·min

-1

，柱温30 ℃，进样量10 μL；采用电喷雾离子源，在正、负离子模式下采集数据。运用Xcalibur 3.0软件，根据化合物的相对保留时间、准分子离子峰和碎片离子峰等信息，结合人类代谢组数据库(HMDB)，参考相关文献以及对照品比对，鉴定三草保肝汤的主要化学成分。

结果：

从三草保肝汤中鉴定出了40个化学成分，包括酚酸类16个、黄酮类19个、蒽醌类2个、三萜类1个、甾醇类1个、单萜类1个，其中有6个化学成分(

-蒎烯、丹参素、表没食子儿茶素、龙胆酸、柚皮素、大黄素)首次从该复方中分离得到。

结论：

UPLC-ESI-HRMS

技术能建立快速、准确分析三草保肝汤中化学成分的方法，对三草保肝汤化学成分进行了较为全面的定性分析，为该复方的药效物质基础研究及质量控制奠定了基础。

Abstract

Objective:

To analysis the chemical constituents in Sancao Baogan decoction by ultra-high performance liquid chromatography coupled with hybrid quadrupole-orbitrap mass spectrometry (UPLC-ESI-HRMS

Method:

The separation was performed on an ACQUITY UPLC BEH C

column (2.1 mm×100 mm

1.7 μm) by a gradient elution of methanol (A)-0.1% formic acid solution (B) (0-2 min

5%A; 2-20 min

5%-12%A; 20-35 min

12%-40%A; 35-38 min

40%A; 38-48 min

40%-80%A; 48-50 min

80%A). The flow rate was 0.3 mL·min

-1

and the column temperature was set at 30 ℃

the injection volume was 10 μL. Electrospray ionization was applied and the data were collected via positive and negative ion modes. By using Xcalibur 3.0 software

the chemical constituents were analyzed based on the relative retention time

excimer ion peak and fragment ion peak of the compounds

as well as comparison with human metabolome database (HMDB)

reference substances and literature data.

Result:

A total of 40 chemical components were identified from Sancao Baogan decoction

including 16 phenolic acids

19 flavonoids

2 anthraquinones

1 triterpenoid

1 sterol

and 1 monoterpenoid. Six compounds (dansensu

-pinene

epigallocatechin

5-dihydroxybenzoic acid

naringenin and emodin) were reported for the first time from Sancao Baogan decoction.

Conclusion:

The established UPLC-ESI-HRMS

can quickly

accurately and comprehensively analyze the chemical constituents of Sancao Baogan decoction

which lays a foundation for the basic research of pharmacodynamic substances and quality control of this formula.

关键词

Keywords

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⁰

Analysis of Chemical Composition in Sancao Baogan Decoction Based on UPLC-ESI-HRMSn

DOI：10.13422/j.cnki.syfjx.20192448

摘要

Abstract

关键词

Keywords

references

Analysis of Chemical Composition in Sancao Baogan Decoction Based on UPLC-ESI-HRMSⁿ