Spectrum-effect Relationship of Anti-hepatoma Activity from 3 Kinds of Genus <italic style="font-style: italic">Paris</italic> Based on Cluster Analysis and Grey Relational Analysis

Ruo-shi LI; Hui-qiong YUAN; Jiang LIU; Fei-ya ZHAO; Ai-en TAO; Bao-zhong DUAN; Ying WANG; Cong-long XIA

doi:10.13422/j.cnki.syfjx.20192449

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Spectrum-effect Relationship of Anti-hepatoma Activity from 3 Kinds of Genus Paris Based on Cluster Analysis and Grey Relational Analysis

Pharmacy Fundamentals | 更新时间：2021-02-09

- Spectrum-effect Relationship of Anti-hepatoma Activity from 3 Kinds of Genus Paris Based on Cluster Analysis and Grey Relational Analysis
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 26, Issue 8, Pages: 192-198(2020)
- 作者机构：
  
  1.大理大学　药学与化学学院，云南省高校滇西道地药材资源开发重点实验室，云南大理　 671000
  2.宜良县第一人民医院，昆明　 652100
  3.云南大学　旅游文化学院，云南丽江　 674100
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20192449
  CLC： R22; R28; Q2; C37; O657.7
- Received：19 June 2019，
  
  Published Online：04 September 2019，
  
  Published：20 April 2020
- 稿件说明：
移动端阅览
Ruo-shi LI, Hui-qiong YUAN, Jiang LIU, et al. Spectrum-effect Relationship of Anti-hepatoma Activity from 3 Kinds of Genus Paris Based on Cluster Analysis and Grey Relational Analysis[J]. Chinese journal of experimental traditional medical formulae, 2020, 26(8): 192-198.
DOI：

Ruo-shi LI, Hui-qiong YUAN, Jiang LIU, et al. Spectrum-effect Relationship of Anti-hepatoma Activity from 3 Kinds of Genus Paris Based on Cluster Analysis and Grey Relational Analysis[J]. Chinese journal of experimental traditional medical formulae, 2020, 26(8): 192-198. DOI： 10.13422/j.cnki.syfjx.20192449.

摘要

目的：

研究长柱重楼、滇重楼和南重楼的HPLC指纹图谱与其抗肝癌作用的谱效关系，为明确重楼抗肝癌作用的物质基础提供实验依据。

方法：

采用HPLC建立3种重楼提取物的指纹图谱，流动相乙腈(A)-水(B)梯度洗脱(0～10 min，20%A；10～20 min，20%～25%A；20～30 min，25%～30%A；30～40 min，30%～35%A；40～50 min，35%～40%A；50～60 min，40%A；60～75 min，40%～45%A；75～80 min，45%～60%A)，流速0.9 mL·min

-1

，检测波长203 nm；利用噻唑蓝(MTT)比色法测定3种重楼提取物对肝癌HepG2细胞的增殖抑制作用，计算半数抑制浓度(IC

)；运用聚类分析(HCA)和灰色关联度分析(GRA)研究3种重楼指纹图谱和抗肝癌作用的关系，找出对抗肝癌作用贡献较大的成分。

结果：

在3种重楼的HPLC指纹图谱中，确定其中11个色谱峰为共有峰。作用时间72 h时长柱重楼、滇重楼、南重楼的IC

分别为148.33，178.87，208.09 mg·L

-1

，其中长柱重楼的抗肝癌活性最强。灰色关联度结果显示，滇重楼共有峰中关联度较高的为1～10号峰，长柱重楼共有峰关联度较高的为1～7号峰，南重楼共有峰中关联度较高的为1～4，6～10，N1号峰，与IC

关联度均

0.7。各重楼变量的聚类分析结果显示，可与IC

聚为一类的色谱峰的关联度均

0.7。

结论：

建立了3种重楼的HPLC指纹图谱，重复性良好。3种重楼中的1～4，6和7号色谱峰对抗肝癌药效贡献最大。

Abstract

Objective:

To study the spectrum-effect relationship between HPLC fingerprints and anti-hepatoma activity of

Paris polyphylla

var.

yunnanensis

forrestii

and

vietnamensis

and to elucidate its effective substance.

Method:

HPLC was used to establish the fingerprint of three extracts from the plant. The mobile phase was consisted of acetonitrile (A)-water (B) for gradient elution (0-10 min

20%A; 10-20 min

20%-25%A; 20-30 min

25%-30%A; 30-40 min

30%-35%A; 40-50 min

35%-40%A; 50-60 min

40%A; 60-75 min

40%-45%A; 75-80 min

45%-60%A)

and the flow rate was 0.9 mL·min

-1

. The UV detection wavelength was 203 nm. Thiazolyl blue tetrazolium bromide (MTT) array was used to detect the inhibitory effects of three extracts on the proliferation of HepG2 cells

and the half inhibitory concentration (IC

) was calculated. Cluster analysis and grey relational analysis were used to analyze the data of spectrum and efficacy

and to find out the components that contributed a lot to the anti-liver cancer effect.

Result:

A total of 11 common peaks were identified as common peaks among HPLC fingerprints of three kinds of

Paris

. After treated 72 h

forrestii

has the highest inhibitory effect on the HepG2 cells

the IC

forrestii

polyphylla

var.

yunnanensis

and

vietnamensis

were 148.33

178.87

208.09 mg·L

-1

respectively. According to the grey relational analysis

the common peaks 1-10 from

polyphylla

var.

yunnanensis

had great correlation to anti-tumor effect

and the common peaks 1-7 for

forrestii

the common peaks 1-4

6-10

N1 for

vietnamensis

all the correlation degrees with IC

were

0.7.Cluster analysis of variables in each

Paris

showed that peaks with correlation degree

0.7 could cluster with IC

Conclusion:

The established HPLC fingerprint method is reliable with good reproducibility. The peaks 1-4

6 and 7 from three kinds of

Paris

have the greatest contribution to the anti-hepatoma effect.

关键词

Keywords

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⁰

Spectrum-effect Relationship of Anti-hepatoma Activity from 3 Kinds of Genus Paris Based on Cluster Analysis and Grey Relational Analysis

DOI：10.13422/j.cnki.syfjx.20192449

摘要

Abstract

关键词

Keywords

references