Anti-depressant Mechanism of Qing' ewan in CUMS Rats Based on Estrogen Receptor Pathway

Shan-shan SONG; Hong SUN; Wen JING; Guo-liang DAI; Wen-zheng JU

doi:10.13422/j.cnki.syfjx.20200103

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Anti-depressant Mechanism of Qing' ewan in CUMS Rats Based on Estrogen Receptor Pathway

Classic Prescriptions | 更新时间：2021-02-09

- Anti-depressant Mechanism of Qing' ewan in CUMS Rats Based on Estrogen Receptor Pathway
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 26, Issue 4, Pages: 9-15(2020)
- 作者机构：
  
  南京中医药大学　附属医院，南京　 210029
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20200103
  CLC： R2-0; R22; R285.5; R289
- Received：13 June 2019，
  
  Published Online：11 September 2019，
  
  Published：20 February 2020
- 稿件说明：
移动端阅览
Shan-shan SONG, Hong SUN, Wen JING, et al. Anti-depressant Mechanism of Qing' ewan in CUMS Rats Based on Estrogen Receptor Pathway[J]. Chinese journal of experimental traditional medical formulae, 2020, 26(4): 9-15.
DOI：

Shan-shan SONG, Hong SUN, Wen JING, et al. Anti-depressant Mechanism of Qing' ewan in CUMS Rats Based on Estrogen Receptor Pathway[J]. Chinese journal of experimental traditional medical formulae, 2020, 26(4): 9-15. DOI： 10.13422/j.cnki.syfjx.20200103.

摘要

目的：

研究青娥丸对慢性温和不可预知应激模型(CUMS)大鼠的抗抑郁效果，及其对雌激素受体及相关信号通路的调控作用，探讨青娥丸的抗抑郁机制。

方法：

54只SD大鼠建立CUMS抑郁大鼠模型，实验设为正常组、模型组、草酸依他普伦组(6.3 mg·kg

-1

)及青娥丸低、中、高剂量组(1.71，5.13，15.39 g·kg

-1

)。CUMS造模4周后给予各组相应药物治疗2周，采用行为学[糖水消耗实验(SPT)，强迫游泳实验(FST)，旷场实验(OFT)]评价大鼠抑郁状态；采用蛋白免疫印迹法(Western blot)检测雌激素受体

(ER

)，雌激素受体

(ER

)，脑源性神经营养因子(BDNF)及酪氨酸激酶受体B(TrkB)的蛋白表达水平。

结果：

与正常组比较，模型组大鼠的糖水消耗率及旷场得分均下降(

0.05，

0.01)，游泳不动时间显著延长(

0.01)，同时ER

，ER

，BDNF和TrkB的蛋白表达水平下降(

0.05，

0.01)；与模型组比较，各给药组大鼠的行为学表现得到改善，糖水消耗率及旷场得分增加(

0.05，

0.01)，游泳不动时间减少(

0.05)，同时ER

，ER

，BDNF和TrkB蛋白的表达明显上调(

0.05，

0.01)，其中青娥丸中剂量组的调节作用更为显著。

结论：

青娥丸可改善CUMS模型大鼠的抑郁样行为，其机制可能与上调ER

，ER

的表达，激活雌激素受体介导的ER

/BDNF/TrkB通路起到神经保护作用有关。

Abstract

Objective:

To study the anti-depressive effect of Qing' ewan in treating chronic unpredictable mild stress (CUMS) in rats

and the regulatory effect on estrogen receptor and estrogen receptor-related signaling pathways

in order to explore its anti-depressive mechanism.

Method:

The CUMS model was established. The experiment was divided into normal control group

model group

escitalopram oxalate group (positive control) and Qing' ewan groups (1.71

5.13

15.39 g·kg

-1

). After 4 weeks of modeling

rats were treated with corresponding drugs for 2 weeks. Behavioral evaluation [sucrose preference test (SPT)

forced swimming test (FST)

open field test (OFT)] was conducted to assess if the CUMS model was successful. Western blot was used to analyze the protein expression levels of estrogen receptor

(ER

)

estrogen receptor

(ER

)

brain-derived neurotrophic factor (BDNF) and tyrosine kinase receptor B (TrkB).

Result:

Compared with the normal group

the sucrose consumption rate and the score of OFT in the model group decreased(

0.05

0.01)

the immobility time of FST prolonged significantly(

0.01)

and the protein expression levels of ER

BDNF and TrkB decreased(

0.05

0.01). Compared with the model group

the behavioral performance of the treated group was improved

the sucrose consumption rate and the score of OFT increased(

0.05

0.01)

and the immobility time decreased(

0.05). The protein expressions of ER

BDNF and TrkB in the treated group were significantly up-regulated(

0.05

0.01)

especially the middle-dose Qing' ewan group (5.13 g·kg

-1

Conclusion:

Qing' ewan can improve depression-like behavior in CUMS rats. Its mechanism may be related to the neuroprotective effect by up-regulating the expressions of ER

and ER

and activating estrogen receptor-mediated ER

/BDNF/TrkB pathways.

关键词

Keywords

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