Investigation of Adsorption Properties of Five Macroporous Resins for Total Flavonoids in Epimedii Folium

Ru YANG; Cong-yan LIU; Ting XU; Rui-yun LI; Yan CHEN

doi:10.13422/j.cnki.syfjx.20200146

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Investigation of Adsorption Properties of Five Macroporous Resins for Total Flavonoids in Epimedii Folium

Pharmacy Fundamentals | 更新时间：2021-02-09

- Investigation of Adsorption Properties of Five Macroporous Resins for Total Flavonoids in Epimedii Folium
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 26, Issue 6, Pages: 113-120(2020)
- 作者机构：
  
  1.南京中医药大学　附属中西医结合医院，南京　 210028
  2.江苏省中医药研究院　中药组分与微生态研究中心，南京　 210028
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20200146
  CLC： R22; R28; O657.7; R94; O643
- Received：16 July 2019，
  
  Published Online：10 September 2019，
  
  Published：20 March 2020
- 稿件说明：
移动端阅览
Ru YANG, Cong-yan LIU, Ting XU, et al. Investigation of Adsorption Properties of Five Macroporous Resins for Total Flavonoids in Epimedii Folium[J]. Chinese journal of experimental traditional medical formulae, 2020, 26(6): 113-120.
DOI：

Ru YANG, Cong-yan LIU, Ting XU, et al. Investigation of Adsorption Properties of Five Macroporous Resins for Total Flavonoids in Epimedii Folium[J]. Chinese journal of experimental traditional medical formulae, 2020, 26(6): 113-120. DOI： 10.13422/j.cnki.syfjx.20200146.

摘要

目的：

比较不同大孔树脂对淫羊藿总黄酮的吸附性能，阐明其吸附机制，优选适合纯化淫羊藿总黄酮的大孔树脂。

方法：

分别以淫羊藿总黄酮和5种代表性成分(朝藿定A，朝藿定B，朝藿定C，淫羊藿苷，宝霍苷Ⅰ)的吸附率、比吸附量、洗脱率、比洗脱量为指标，采用静态吸附和动态吸附相结合的方法，比较HPD100，HPD600，AB-8，X-5，D101共5种型号大孔树脂的吸附特性；利用伪一阶、伪二阶动力学模型和颗粒内扩散模型对优选树脂的吸附动力学进行研究，运用Langmuir和Freundlich等温吸附模型对优选树脂的热力学过程进行分析，从物理化学角度探究树脂的吸附机制。

结果：

HPD100型大孔吸附树脂对淫羊藿总黄酮的吸附和洗脱性能均较好，显著优于其他树脂；其对淫羊藿总黄酮及5种代表性黄酮的吸附动力学过程均符合伪二阶动力学模型，对淫羊藿总黄酮的吸附热力学过程符合Freundlich模型，对5种黄酮类成分之和的吸附热力学过程符合Langmuir模型；HPD100型大孔树脂对淫羊藿总黄酮的吸附过程为物理吸附占主导的放热过程，最适吸附温度25 ℃。

结论：

HPD100型大孔树脂吸附容量大、洗脱顺畅，吸附机制明确，适用于淫羊藿总黄酮的分离与纯化。

Abstract

Objective:

To compare the adsorption characteristics of different macroporous adsorption resins for the total flavonoids in Epimedii Folium

clarify the adsorption mechanism

and screen the optimal resin for the purification of total flavonoids in Epimedii Folium.

Method:

Taking the adsorption and desorption capacities of the total flavonoids in Epimedii Folium and five representative flavonoids (epimedin A

epimendin B

epimendin C

icariin

baohuoside Ⅰ) as indexes

static adsorption and dynamic adsorption experiments were conducted to compare the adsorption characteristics of five macroporous including HPD100

HPD600

AB-8

X-5 and D101.The adsorption kinetics of the selected resin was studied by using the pseudo-first-order

pseudo-second-order kinetic models and intraparticle diffusion model

and the thermodynamic process was analyzed by using the Langmuir and Freundlich isothermal adsorption models

which explored the adsorption mechanism of resin from the perspective of physical chemistry.

Result:

HPD100 macroporous resin had a better adsorption and desorption properties than the others. The adsorption process of HPD100 macroporous resin for total flavonoids in Epimedii Folium and five representative flavonoids conformed to the pseudo-second-order kinetic model. The adsorption thermodynamic process of HPD100 resin for total flavonoids of Epimedii Folium conformed to the Freundlich model

and for the sum of five representative flavonoids conformed to the Langmuir model. The adsorption process of HPD100 resin for total flavonoids in Epimedii Folium was the exothermic process dominated by physical adsorption

and the optimal adsorption temperature was 25 ℃.

Conclusion:

HPD100 macroporous resin has large adsorption capacity

easy desorption and clear adsorption mechanism

it is suitable for isolation and purification of total flavonoids in Epimedii Folium.

关键词

Keywords

references

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Related Institution

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