Analysis of Grading Evaluation Standard of Ophiopogonis Radix from Sichuan Province

Hui WANG; Kun CHANG; Peng GAO; Song-bo QU; Yuan ZHANG; Ming-bo ZHAO; Chuan-kun SHEN; Zhi-yong LE; Yong JIANG; Peng-fei TU; Xiao-yu GUO

doi:10.13422/j.cnki.syfjx.20200147

您当前的位置：

首页 >

文章列表页 >

Analysis of Grading Evaluation Standard of Ophiopogonis Radix from Sichuan Province

Pharmacy Fundamentals | 更新时间：2021-02-09

- Analysis of Grading Evaluation Standard of Ophiopogonis Radix from Sichuan Province
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 26, Issue 9, Pages: 194-201(2020)
- 作者机构：
  
  1.北京大学　药学院，天然药物及仿生药物国家重点实验室，北京　 100191
  2.北京中医药大学　中药学院，北京　 102488
  3.四川代代为本农业科技有限公司，四川绵阳　 621100
  4.康美药业股份有限公司，广东普宁　 515300
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20200147
  CLC： R22; R28; C37; R931; O657.7
- Received：03 September 2019，
  
  Published Online：09 October 2019，
  
  Published：05 May 2020
- 稿件说明：
移动端阅览
Hui WANG, Kun CHANG, Peng GAO, et al. Analysis of Grading Evaluation Standard of Ophiopogonis Radix from Sichuan Province[J]. Chinese journal of experimental traditional medical formulae, 2020, 26(9): 194-201.
DOI：

Hui WANG, Kun CHANG, Peng GAO, et al. Analysis of Grading Evaluation Standard of Ophiopogonis Radix from Sichuan Province[J]. Chinese journal of experimental traditional medical formulae, 2020, 26(9): 194-201. DOI： 10.13422/j.cnki.syfjx.20200147.

摘要

目的：

通过对川麦冬药材多种质控指标的检测和分析，探索该药材性状与内在质量之间的关联性，为川麦冬药材等级标准的制定提供参考。

方法：

对28批不同等级川麦冬药材(一等品、二等品、三等品和统货)开展性状检查、显微和薄层鉴别、水分、灰分、酸不溶性灰分、二氧化硫残留量、重金属和有害元素残留量、多效唑残留量、水溶性浸出物、总皂苷含量及3种主要成分(麦冬皂苷D，甲基麦冬黄烷酮A，甲基麦冬黄烷酮B)含量测定，分析各项指标与川麦冬药材等级之间的相关性。

结果：

川麦冬药材在性状、显微特征和薄层色谱方面均具有专属性特征，川麦冬药材的杂质、水分、灰分、酸不溶性灰分和水溶性浸出物在不同等级之间存在一定差异性。川麦冬一等品每50 g药材含80～120粒，水分11.1%～14.9%，灰分1.6%～2.1%，酸不溶性灰分0.03%～0.14%，水溶性浸出物77.0%～86.5%；二等品每50 g药材含120～160粒，水分13.1%～14.2%，灰分1.3%～2.2%，酸不溶性灰分0.06%～0.22%，水溶性浸出物75.9%～83.3%；三等品每50 g药材含160～300粒，杂质0.2%～8.4%，水分12.6%～14.0%，灰分1.2%～1.5%，酸不溶性灰分0.06%～0.22%，水溶性浸出物74.0%～86.5%；统货每50 g药材含80～300粒，杂质1.2%～22.6%，水分13.0%～15.4%，灰分1.4%～2.0%，酸不溶性灰分0.03%～0.15%，水溶性浸出物79.8%～85.2%。

结论：

川麦冬药材以每50 g所含粒数等外观性状并结合杂质、浸出物等内在指标进行等级划分具有一定合理性和可行性，可作为川麦冬药材等级标准的参考。

Abstract

Objective:

To investigate the correlation between the characteristics and internal quality of Ophiopogonis Radix from Sichuan province through various quality evaluation

and provide reference for the formulation of grading standard of this herb.

Method:

For 28 batches of Ophiopogonis Radix

the characters

microscopic characteristics

thin-layer chromatography (TLC)

the content of moisture

ash content

acid insoluble ash content

residues of sulfur dioxide

heavy metals

hazardous elements and paclobutrazol

water-soluble extract

contents of total saponins and three major components [ophiopogonin D (OPD)

methylophiopogonanone A (MPOA) and methylophiopogonanone B (MPOB)] were determined.The relationships between various indicators and the grade of Ophiopogonis Radix were analyzed.

Result:

Ophiopogonis Radix exhibited specific properties in characters

microscopic characteristics and TLC.The contents of impurity

moisture

total ash

acid-insoluble ash and water-soluble extract existed differences in four grades of Ophiopogonis Radix from Sichuan province.For grade Ⅰ

grain number was 80-120 grains per 50 g

the moisture content was 11.1%-14.9%

total ash content was 1.6%-2.1%

acid-insoluble ash content was 0.03%-0.14%

water-soluble extract content was 77.0%-86.5%.For grade Ⅱ

grain number was 120-160 grains per 50 g

the moisture content was 13.1%-14.2%

total ash content was 1.3%-2.2%

acid-insoluble ash content was 0.06%-0.22%

water-soluble extract content was 75.9%-83.3%.For grade Ⅲ

grain number was 160-300 grains per 50 g

the impurity was 0.2%-8.4%

the moisture content was 12.6%-14.0%

total ash content was 1.2%-1.5%

acid-insoluble ash content was 0.06%-0.22%

water-soluble extract content was 74.0%-86.5%.For grade Ⅳ

grain number was 80-300 grains per 50 g

impurity was 1.2%-22.6%

the moisture content was 13.0%-15.4%

the total ash content was 1.4%-2.0%

acid-insoluble ash content was 0.03%-0.15%

water-soluble extract content was 79.8%-85.2%.

Conclusion:

It is reasonable and feasible to classify the grade of Ophiopogonis Radix from Sichuan province according to the appearance characteristics such as the grain number per 50 g and internal indexes such as impurity and water-soluble extract

which can be used as a reference for grading standards of Ophiopogonis Radix from Sichuan province.

关键词

Keywords

references

国家药典委员会．中华人民共和国药典：一部 [M]．北京：中国医药科技出版社， 2015 ： 155 - 156 .

S WANG , Z ZHANG , X LIN , et al . A polysaccharide，MDG-1，induces S1P1 and bFGF expression and augments survival and angiogenesis in the ischemic heart [J]． Glycobiology ， 2010 ， 20 ( 4 )： 473 - 484 .

C X YAO , X L SHI , X LIN , et al . Increased cardiac distribution of mono-PEGylated Radix Ophiopogonis polysaccharide in both myocardial infarction and ischemia/reperfusion rats [J]． Int J Nanomedicine ， 2015 ， 10 ： 409 - 418 .

P B LI , W L LIN , Y G WANG , et al . Antidiabetic activities of oligosaccharides of Ophiopogonis japonicus in experimental type 2 diabetic rats [J]． Int J Biol Macromol ， 2012 ， 51 ( 5 )： 749 - 755 .

L Q BI , R ZHU , H KONG , et al . Ruscogenin attenuates monocrotaline-induced pulmonary hypertension in rats [J]． Int Immunopharmacol ， 2013 ， 16 ( 1 )： 7 - 16 .

L SUN , S LIN , R ZHAO , et al . The Saponin monomer of dwarf Lilyturf tuber，DT-13，reduces human breast cancer cell adhesion and migration during hypoxia via regulation of tissue factor [J]． Biol Pharm Bull ， 2010 ， 33 ( 7 )： 1192 - 1198 .

景晓琦，陈彤垚，张福生，等．不同商品等级远志药材的HPLC指纹图谱分析 [J]．中国实验方剂学杂志， 2018 ， 24 ( 24 )： 31 - 37 .

蔡广知，赵凌，王莎莎，等．甘草药材等级标准分析 [J]．中国实验方剂学杂志， 2019 ， 25 ( 10 )： 148 - 153 .

孙红祥，曾宪武．麦冬类药材质量的综合评价 [J]．中草药， 2001 ， 32 ( 5 )： 313 - 315 .

国家药典委员会．中华人民共和国药典：四部 [M]．北京：中国医药科技出版社， 2015 ： 43-44，101，103-104，202，204，208，404 .

刘霞，林韵涵，谢彩香，等．道地药材川麦冬和浙麦冬的生态遗传分化 [J]．中国实验方剂学杂志， 2017 ， 23 ( 17 )： 27 - 33 .

吴发明，杨瑞山，陶玲，等．基于药材安全性和有效性的综合评价探讨多效唑在麦冬中的应用 [J]．中国药学杂志， 2017 ， 52 ( 1 )： 20 - 24 .

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Mechanism of Action of Coptidis Rhizoma and Ophiopogonis Radix in Delaying Diabetic Nephropathy Based on EGFR/PI3K/Akt Signaling Pathway

Origin，Processing Method and Quality Standard of Fermentum Rubrum： A Review

Analysis on Determination and Quantity Transfer of Standard Decoction of Ginseng Radix et Rhizoma by Fresh and Traditional Cutting

Optimization of Decoction Technology and Establishment of Quality Standard for Moringa oleifera Leaf Standard Decoction

Related Author

LI Shaoyu

GONG Man

LI Qiufang

DAI Liping

WANG Guiqun

YANG Qiuchen

ZHANG Qiongqiong

XU Erping

Related Institution

Henan Collaborative Innovation Center for Research and Development on the Whole Industry Chain of Yu-Yao，Henan University of Chinese Medicine

Academy of Chinese Medical Sciences， Henan University of Chinese Medicine

School of Pharmacy， Henan University of Chinese Medicine

Reproductive Health Hospital of the Third Affiliated Hospital of Zhengzhou University

Jiangxi University of Chinese Medicine

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰