Effect and Mechanism of Buyang Huanwu Tang on Atherosclerotic Plaque Based on Treg Cells

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Effect and Mechanism of Buyang Huanwu Tang on Atherosclerotic Plaque Based on Treg Cells

Classic Prescriptions | 更新时间：2021-02-09

- Effect and Mechanism of Buyang Huanwu Tang on Atherosclerotic Plaque Based on Treg Cells
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 26, Issue 5, Pages: 1-5(2020)
- 作者机构：
  
  天津中医药大学，天津　 301617
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20200201
  CLC： R2-0; R22; R285.5; R289
- Received：01 July 2019，
  
  Published Online：09 October 2019，
  
  Published：05 March 2020
- 稿件说明：
移动端阅览
Xiao-li PANG, Qing-long WANG, Shu-jing CHEN, et al. Effect and Mechanism of Buyang Huanwu Tang on Atherosclerotic Plaque Based on Treg Cells[J]. Chinese journal of experimental traditional medical formulae, 2020, 26(5): 1-5.
DOI：

Xiao-li PANG, Qing-long WANG, Shu-jing CHEN, et al. Effect and Mechanism of Buyang Huanwu Tang on Atherosclerotic Plaque Based on Treg Cells[J]. Chinese journal of experimental traditional medical formulae, 2020, 26(5): 1-5. DOI： 10.13422/j.cnki.syfjx.20200201.

摘要

目的：

2

从调节性T细胞(Treg)细胞和炎症角度探讨补阳还五汤(BYHWT)对动脉粥样硬化斑块影响及具体作用机制。

方法：

2

8周龄50只ApoE基因敲除(ApoE

-/-

)小鼠随机分为模型组，BYHWT组低、中、高剂量，雷帕霉素组，以C57/BL小鼠作为正常组。模型组，BYHWT组给予高脂饮食造模12周，正常组给予正常饮食。造模成功后，BYHWT低、中、高剂量组分别给予5，10，20 g·kg

-1

BYHWT灌胃，雷帕霉素组给予促进Treg细胞增殖剂-雷帕霉素(4 mg·kg

-1

)，正常组和模型组给予等剂量生理盐水灌胃，所有组灌胃4周；4周后取心脏主动脉窦，制备成石蜡组织切片，采用苏木素-伊红(HE)染色法评价斑块面积，采用免疫组化评估斑块内Treg细胞表达数量。眼内眦取血，采用酶联免疫吸附测定(ELISA)法检测外周血炎症因子表达，采用实时荧光定量聚合酶链式反应(Real-time PCR)检测外周血叉状头转录因子p3(Foxp3) mRNA表达。

结果：

2

与正常组比较，模型组动脉粥样硬化斑块面积显著增加(

P

<

0.01)，外周血中肿瘤坏死因子-

α

(TNF-

α

)，白细胞介素-6(IL-6)含量均显著升高(

P

<

0.05)，转化生长因子-

β

(TGF-

β

)，白细胞介素-10(IL-10)明显降低(

P

<

0.05)，外周血Foxp3 mRNA表达明显降低(

P

<

0.05)；与模型组相比，BYHWT中、高剂量组斑块面积明显减小(

P

<

0.05)，外周血TNF-

α

，IL-6含量降低(

P

<

0.01)，BYHWT高剂量组TGF-

β

，IL-10表达增加(

P

<

0.05，

P

<

0.01)，高剂量组斑块内Treg细胞表达增加(

P

<

0.01)，各剂量组外周血Fxop3 mRNA表达增加(

P

<

0.01)。

结论：

2

BYHWT具有抗动脉粥样硬化作用，该作用可能与增加Treg细胞数量，进而抑制体内炎症反应有关。

Abstract

Objective:

2

To explore the effect and mechanisms of Buyang Huanwu Tang (BYHWT) on atherosclerotic plaque based on regulatory T cells (Treg) and inflammation.

Method:

2

Totally 50 ApoE knockout(ApoE

-/-

)mice aged 8 weeks were randomly divided into model group

low

medium

high-dose BYHWT groups

positive control group

and C57/BL mice were taken as control group. The model group and the BYHWT group were given high-fat diet for 12 weeks

while the control group was given normal diet. After successful modeling

BYHWT groups were given drugs (5

10

20 g·kg

-1

) through intragastric administration

the positive control group was given rapamycin (4 mg·kg

-1

)

while the control group and the model group were given equal doses normal saline through intragastric administration for 4 weeks. Four weeks later

the mice were sacrificed. Manufactured paraffin sections were prepared for the aortic sinus of the heart. The plaque area was evaluated by hematoxylin and eosin (HE) staining

and the number of Treg cells in immunohistochemical staining plaque was detected. Blood was collected from eye canthus of mice

the expression of inflammatory factors in peripheral blood was detected by enzyme-linked immunosorbent assay (ELISA)

and the forkhead box P3 (Foxp3) gene expression in peripheral blood was detected by Real-time fluorescent quantitative polymerase chain reaction (PCR).

Result:

2

Compared with the control group

the area of atherosclerotic plaques in the model group was significantly increased (

P

<

0.01)

the contents of serum tumor necrosis factor-

α

(TNF-

α

) and interleukin-6 (IL-6) were significantly increased (

P

<

0.05)

and transforming growth factor-

β

(TGF-

β

) and interleukin-10 (IL-10) were significantly decreased (

P

<

0.05)

and the peripheral blood Fxop3 mRNA expression was decreased (

P

<

0.05). Compared with the model group

the plaque areas in middle-dose and high-dose BYHWT groups were significantly reduced(

P

<

0.05)

the peripheral blood TNF-

α

and IL-6 contents were decreased (

P

<

0.01)

the TGF-

β

and IL-10 expressions were increased in the high-dose group (

P

<

0.05

P

<

0.01)

the number of Treg cells in the plaque was increased in the high-dose group (

P

<

0.01)

and the peripheral blood Fxop3 mRNA expression was increased in each BYHWT group (

P

<

0.01).

Conclusion:

2

BYHWT has an anti-atherosclerosis effect

which may be related to the increase of the number of Treg cells and thereby inhibiting the inflammatory response

in vivo

.

关键词

Keywords

references

赵帅，张烈元，冯文伟，等 . 疏肝温胆汤对动脉粥样硬化家兔LXR α ，NF-кB及内皮功能的影响 [J]. 中国实验方剂学杂志， 2019 ， 25 ( 15 ): 108 - 115 .

K MITEVA , R MADONNA , R DE CATERINA , et al . Innate and adaptive immunity in athero-sclerosis [J]. Vascul Pharmacol , 2018 ， 107 : 67 - 77 .

F ADBOLMALEKI , S M GHEIBI HAYAT , V BIANCONI , et al . Atherosclerosis and immunity: a perspective [J]. Trends Cardiovasc Med ， 2018 ， 8 : 1 - 9 .

P SIMA , L VANNUCCI , V VETVICKA . Atherosclerosis as autoimmune disease [J]． Ann Transl Med , 2018 ， 6 ( 7 ): 1 - 6 .

刘玉晖，侯贝贝，游宇，等 . 补阳还五汤稳定ApoE -/- 小鼠动脉粥样硬化易损斑块的作用机制 [J]. 中国实验方剂学杂志， 2018 ， 24 ( 15 ): 112 - 119 .

刘楠，姜云耀，黄婷婷，等．基于网络药理学方法研究补阳还五汤治疗脑梗死的作用机制 [J]．中国中药杂志， 2018 ， 43 ( 11 ): 2190 - 2198 .

黄兴，李艳芬，寇冠军，等．补阳还五汤抗动脉粥样硬化作用机制研究进展 [J]. 中华中医药杂志， 2017 ， 32 ( 3 ): 1187 - 1190 .

路永坤，杨海燕，刘向哲，等．补阳还五汤佐治超早期脑梗死患者对静脉溶栓后出血性转化的影响 [J]．中国中药杂志， 2019 ， 44 ( 8 ): 1696 - 1703 .

万强，刘中勇．定心方通过上调CD4 ＋ CD25 ＋ Foxp3 ＋调节性T细胞表达对PM2.5所致动脉粥样硬化的影响 [J]. 中国实验方剂学杂志， 2018 ， 24 ( 17 ): 139 - 144 .

C F AMANDA , H L ANDREW , K JOHAN ． Treating atherosclerosis with regulatory T cells [J]. Arterioscler Thromb Vasc Biol ， 2015 ， 35 ( 2 ): 280 - 287 .

邓中甲．方剂学 [M]．北京：中国中医药出版社， 2011 ： 240 - 241 .

Y H ZHU , X M XIAN , Z Z WANG , et al . Research progress on the relationship between atherosclerosis and inflammation [J]. Biomolecules , 2018 ， 8 ( 3 ): 1 - 11 .

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Related Institution

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