Molecular Cloning and Sequence Analysis of <italic style="font-style: italic">GgUGE</italic> Gene in <italic style="font-style: italic">Glycyrrhiza glabra</italic>

Jia-ming HOU; Shao-kai TIAN; Lin YANG; Zhi-xin ZHANG; Zhi-qiang GAO; Han-qing WANG; Ying LIU

doi:10.13422/j.cnki.syfjx.20200214

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Molecular Cloning and Sequence Analysis of GgUGE Gene in Glycyrrhiza glabra

Pharmacy Fundamentals | 更新时间：2021-02-09

- Molecular Cloning and Sequence Analysis of GgUGE Gene in Glycyrrhiza glabra
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 26, Issue 6, Pages: 163-167(2020)
- 作者机构：
  
  1.北京中医药大学　生命科学学院，北京　 102488
  2.宁夏医科大学　药学院，银川　 750004
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20200214
  CLC： R284.2; R289; R22; R2-031
- Received：29 April 2019，
  
  Published Online：11 October 2019，
  
  Published：20 March 2020
- 稿件说明：
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Jia-ming HOU, Shao-kai TIAN, Lin YANG, et al. Molecular Cloning and Sequence Analysis of GgUGE Gene in Glycyrrhiza glabra[J]. Chinese journal of experimental traditional medical formulae, 2020, 26(6): 163-167.
DOI：

Jia-ming HOU, Shao-kai TIAN, Lin YANG, et al. Molecular Cloning and Sequence Analysis of GgUGE Gene in Glycyrrhiza glabra[J]. Chinese journal of experimental traditional medical formulae, 2020, 26(6): 163-167. DOI： 10.13422/j.cnki.syfjx.20200214.

摘要

目的：

通过克隆光果甘草

Glycyrrhiza glabra

UDP-葡萄糖4-差向异构酶(UDP-glucose 4-epimerase，

UGE

)基因并进行生物信息学分析，探究光果甘草

UGE

基因与甘草酸生物合成分子调控之间的潜在关系。

方法：

从光果甘草主根中提取RNA，通过逆转录聚合酶链式反应(RT-PCR)克隆得到

UGE

基因cDNA序列，测序并进行序列分析。

结果：

成功克隆得到一条长度为1 121 bp的光果甘草

UGE

基因(

GgUGE

)cDNA序列，包含1个长度为1 053 bp的开放阅读框(ORF)，共编码350个氨基酸残基，在GenBank上对所得cDNA序列进行注册，序列注册号为MK638908。序列分析表明

GgUGE

基因编码蛋白为不稳定亲水蛋白，理论相对分子质量为39.02 kDa，等电点为6.13，不含信号肽，无跨膜区，二级结构以

-螺旋为主，保守结构域含有葡萄糖4-差向异构酶基因家族结构域。

GgUGE

基因的cDNA序列和氨基酸序列聚类分析结果表明其与豆科植物亲缘关系最近，而与杨柳科植物亲缘关系较远。

结论：

首次克隆得到了光果甘草

GgUGE

cDNA序列，对其进行了生物信息学分析，为后续

GgUGE

基因的功能研究以及甘草酸生物合成分子调控机制的解析提供参考。

Abstract

Objective:

To clone the cDNA sequence of UDP-glucose 4-epimerase (UGE) in

Glycyrrhiza glabra

and analyze its sequence

so as to explore the potential relationship between the

UGE

gene and the molecular regulatory mechanisms of glycyrrhizic acid biosynthesis.

Method:

The cDNA sequence of

UGE

was cloned from the root of

glabra

by reverse transcription polymerase chain reaction (RT-PCR)

then sequenced and analyzed by bioinformatics software.

Results:

GgUGE

cDNA sequence with the full length of 1 121 bp was obtained. The open reading fame (ORF) of

GgUGE

was 1 053 bp

encoding 350 amino acid residues. The

GgUGE

cDNA sequence was submitted to GenBank

and the accession No. was MK638908. Sequence analysis showed that GgUGE was an unstable hydrophilic protein

its average relative molecular weight was 39.02 kDa

and isoelectric point was 6.13. It contained no signal peptides or transmembrane domains. Its secondary structure mainly constituted of

-helix and had a conversed domain of UDP-glucose 4-epimerase superfamily. The homologoue analysis showed that the cDNA and amino acid sequences of

GgUGE

had the closest evolutionary relationship to Leguminosae and relatively distant evolutionary relationship to Salicaceae.

Conclusion:

In this study

GgUGE cDNA

sequence is successfully cloned from

glabra

for the very first time

which will provide reference for studying the function of

GgUGE

and explaining the molecular regulatory mechanisms of glycyrrhizic acid biosynthesis in

glabra

关键词

Keywords

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Molecular Cloning and Sequence Analysis of GgUGE Gene in Glycyrrhiza glabra

DOI：10.13422/j.cnki.syfjx.20200214

摘要

Abstract

关键词

Keywords

references