Effect of Evodia on Lipid Metabolism in Hyperlipidemia Mice

Yi-ying YANG; Ming LIU; Jing-ye HU; Man ZHANG; Ying-ting LUO; Hui-yun YANG; Xiao-qin ZHAO

doi:10.13422/j.cnki.syfjx.20200302

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Effect of Evodia on Lipid Metabolism in Hyperlipidemia Mice

Pharmacology | 更新时间：2021-02-09

- Effect of Evodia on Lipid Metabolism in Hyperlipidemia Mice
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 26, Issue 6, Pages: 46-51(2020)
- 作者机构：
  
  贵州中医药大学，贵阳　 550025
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20200302
  CLC： R2-0; R22; R285.5
- Received：03 July 2019，
  
  Published Online：21 October 2019，
  
  Published：20 March 2020
- 稿件说明：
移动端阅览
Yi-ying YANG, Ming LIU, Jing-ye HU, et al. Effect of Evodia on Lipid Metabolism in Hyperlipidemia Mice[J]. Chinese journal of experimental traditional medical formulae, 2020, 26(6): 46-51.
DOI：

Yi-ying YANG, Ming LIU, Jing-ye HU, et al. Effect of Evodia on Lipid Metabolism in Hyperlipidemia Mice[J]. Chinese journal of experimental traditional medical formulae, 2020, 26(6): 46-51. DOI： 10.13422/j.cnki.syfjx.20200302.

摘要

目的：

研究吴茱萸碱对高脂血症小鼠脂质代谢及低密度脂蛋白-受体(LDL-R)mRNA表达的影响。

方法：

昆明种小鼠(

＝80)随机分为正常组(

＝20)和模型组(

＝60)。模型组高脂饲料饲养3周后检测血脂，造模成功后随机分每5组，每组10只，模型组(等量生理盐水)、阳性药组(辛伐他汀5 mg·kg

-1

)，给药组(吴茱萸碱5.25，10.5，21 mg·kg

-1

)，给药3周。苏木素-伊红(HE)染色切片观察小鼠肝细胞结构及主动脉弓粥样改变程度，用酶联免疫试剂盒测试各小鼠血清中总胆固醇(TC)，甘油三酯(TG)，高密度脂蛋白胆固醇(HDL-C)，低密度脂蛋白胆固醇(LDL-C)与血清总脂联素(ADPN)的含量。采用逆转录-聚合酶链式反应( RT-PCR)的方法，检测各组小鼠肝脏LDL-R mRNA的表达。

结果：

HE染色切片可见模型组小鼠肝细胞肿胀并伴有脂肪变性，吴茱萸碱高、中剂量及辛伐他汀组可改善肝脏脂肪变性。模型组小鼠主动脉弓管壁有损伤、有明显内膜增厚现象、炎细胞浸润；吴茱萸碱低剂量组内膜增厚明显，高剂量组小鼠主动脉血管壁结构较清晰，少见内皮损伤。与正常组比较，模型组小鼠血清中TC，TG，LDL-C升高，HDL-C水平降低(

0.01)；与模型组比较，吴茱萸碱中、高剂量组小鼠血清TC，TG水平降低，吴茱萸碱低、中、高剂量组LDL-C降低，HDL-C水平升高(

0.05，

0.01)。与正常组比较，模型组脂联素水平降低，与模型组比较，吴茱萸碱中、高剂量组的血清脂联素水平明显升高(

0.01)。与正常组比较，模型组小鼠肝脏LDL-R mRNA的表达明显降低(

0.01)；与模型组比较，吴茱萸碱中、高剂量组LDL-R mRNA表达明显增强(

0.01)。

结论：

吴茱萸碱能改善高脂血症小鼠肝脏病变、主动脉粥样硬化趋势，可能与通过调节脂联素水平，降低小鼠血脂含量及上调小鼠肝脏LDL-R mRNA的表达有关。

Abstract

Objective:

To study the effect of evodia on lipid metabolism and low-density lipoprotein-receptor(LDL-R) mRNA expression in hyperlipidemia mice.

Method:

Kunming mice (

=80) were randomly divided into normal control group (

=20) and model group (

=60). Serum lipids of the model group were measured after 3 weeks.After successful modeling

the mice can be randomly divided into 5 groups (with 10 in each group): model group (equivalent normal saline)

positive control group (simvastatin

5 mg·kg

-1

·d

-1

)

drug group (evodia of 5.25

10.5

21 mg·kg

- 1

·d

- 1

). The mice were given drugs for 3 weeks.Htoxylin-eosin(HE) staining was used to observe the liver cell structure and the change of aortic arch atherosclerosis in the mice.The enzyme linked immunosorbent assay kit was used to test the contents of total cholesterol (TC)

triglyceride (TG)

high-density lipoprotein cholesterol (HDL-C)

low-density lipoprotein cholesterol (LDL-C) and total serum adiponectin (ADPN) in serum of the mice.The expression of LDL-R mRNA in liver of each group was detected by reverse transcription-polymerase chain reaction (RT-PCR).

Result:

Liver HE staining showed hepatocyte swelling with steatosis in the model group

and alleviated liver steatosis in high-dose

medium-dose evodia and simvastatin groups.HE staining showed damages on the aortict arch wall in the model group

with obvious intima thickening and inflammatory cell infiltration.The intima was thickened obviously in the low-dose group

and the structure of aortic vessel wall was clear in the high-dose group.Compared with the normal group

TG and HDL-C levels in serum of the model group were increased

while HDL-C level was decreased (

0.01). Serum TC and TG levels of mice in the medium and high-dose groups decreased

whereas LDL-C and HDLl-C levels increased in low

medium and high-dose groups (

0.05

0.01). Compared with the normal group

the adiponectin level in the model group was decreased

while the serum adiponectin levels in medium and high-dose groups were significantly increased (

0.01). The LDL-R mRNA expression in the liver of mice in the model group was significantly reduced compared with the normal group (

0.01). The LDL-R mRNA expression in medium and high-dose evodia groups was significantly increased compared with the model group (

0.01).

Conclusion:

Evodia can improve the tendency of hepatic lesions and aortic atherosclerosis in hyperlipidemia mice

which may be related to the regulation of adiponectin level

the reduction of lipid content in mice and the up-regulation of LDL-R mRNA expression in mice liver.

关键词

Keywords

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