Chemical Constituents of Ginseng Radix et Rhizoma with Different Growth Years and Different Origins Based on LC-MS

Bo ZHANG; Xiu-li SUN; Yun-long GUO; Yang WANG; Shu-ying LIU

doi:10.13422/j.cnki.syfjx.20200315

您当前的位置：

首页 >

文章列表页 >

Chemical Constituents of Ginseng Radix et Rhizoma with Different Growth Years and Different Origins Based on LC-MS

Pharmacy Fundamentals | 更新时间：2021-02-09

- Chemical Constituents of Ginseng Radix et Rhizoma with Different Growth Years and Different Origins Based on LC-MS
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 26, Issue 8, Pages: 206-212(2020)
- 作者机构：
  
  长春中医药大学　吉林省人参科学研究院，长春　 130117
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20200315
  CLC： R289; R284.1; R22; R2-031
- Received：21 March 2019，
  
  Published Online：17 October 2019，
  
  Published：20 April 2020
- 稿件说明：
移动端阅览
Bo ZHANG, Xiu-li SUN, Yun-long GUO, et al. Chemical Constituents of Ginseng Radix et Rhizoma with Different Growth Years and Different Origins Based on LC-MS[J]. Chinese journal of experimental traditional medical formulae, 2020, 26(8): 206-212.
DOI：

Bo ZHANG, Xiu-li SUN, Yun-long GUO, et al. Chemical Constituents of Ginseng Radix et Rhizoma with Different Growth Years and Different Origins Based on LC-MS[J]. Chinese journal of experimental traditional medical formulae, 2020, 26(8): 206-212. DOI： 10.13422/j.cnki.syfjx.20200315.

摘要

目的：

对不同年限、不同产地人参的化学成分进行分析研究。

方法：

利用快速分离液相色谱-飞行时间质谱(RRLC-Q-TOF-MS)对样本进行检测，经过色谱峰提取、排列和归一化后，对得到的数据集进行数据统计分析，寻找差异成分，并观察其在不同人参样本中的变化趋势；使用一级质谱和二级质谱信息结合对照品比对进行化合物鉴定。

结果：

偏最小二乘得分图中不同产地和不同生长年限的人参被分开，说明不同产地和不同生长年限的人参中所含的化学成分存在差异，选择变量权重(VIP)值

1的变量作为对分类贡献较大的化学成分，然后进行

检验进一步筛选具有显著性差异(

0.05)的化学成分。对比三年生和五年生人参的化学成分，人参皂苷Rb

，Rh

，Rk

等11种成分存在显著性差异，这些成分中大部分的含量会随着人参生长年限的增加而增加；人参皂苷Rg

，Rf，Rh

，Rb

等10成分在产地为吉林和黑龙江的人参样本中存在显著性差异。

结论：

液质联用技术可以快速、准确地对人参样品进行分析，结合多元统计分析方法，能够寻找不同样本间的差异成分。

Abstract

Objective:

To study the components of Ginseng Radix et Rhizoma of different origins and growth years.

Method:

Rapid liquid chromatography-time of flight mass spectrometry (RRLC-Q-TOF-MS) was applied to detect the raw data of Ginseng Radix et Rhizoma.After peak extraction

alignment

and normalization

the multivariate statistical analysis was made for the resulted dataset to find out the different compounds.The compounds were identified by using accurate molecular weight and tandem mass spectra

and the standard references were used to further confirm the identification.The changing trends of these components in different ginseng samples were observed.

Result:

The ginseng samples of different growth years and different origins were divided into different groups in the score plot of PLS-DA

and the variables with the variable importance in projection (VIP) value of more than 1 were considered to contribute more to the separations

then the

-test was applied to determine whether potential biomarkers were statistically significant (

0.05) between the two groups.The contents of eleven compounds

including ginsenoside Rb

and Rk

were significantly different between ginseng samples aged 3 and 5 years

and the contents of these compounds increased as the rise of the ginseng growth years.Ginsenosides Rg

and other six compounds were significantly different in ginseng samples from Jilin and Heilongjiang province.

Conclusion:

LC-MS is a rapid and accurate method for the analysis of ginseng samples

and could help to find out the different components among samples.

关键词

Keywords

references

C I COLEMAN , J H HEBERT , P REDDY ． The effects of Panax ginseng on quality of life [J]． J Clin Pharm Therapeutics ， 2003 ， 28 ( 1 )： 5 - 15 .

D O KENNEDY , A B SCHOLEY ． Ginseng: potential for the enhancement of cognitive performance and mood [J]． Pharmacol Biochem Behavior ， 2003 ， 75 ( 3 )： 687 - 700 .

闫克敏，肖海娟，杨林，等．人参皂苷Rh 2 对结肠癌耐药细胞HCT116/L-OHP侵袭迁移能力的影响 [J]．中国实验方剂学杂志， 2019 ， 25 ( 13 )： 73 - 78 .

赵自明，潘华山，冯毅翀．人参皂苷Rg 1 抗氧化能力的实验研究 [J]．江西中医学院学报， 2009 ， 21 ( 1 )： 36 - 38 .

江昌，缪雨青，周文丽，等．人参皂苷Rg 3 的抗肿瘤作用及研究进展 [J]．临床肿瘤学杂志， 2017 ， 22 ( 7 )： 664 - 667 .

杨蕾，张振海，贾晓斌．人参稀有皂苷组分联合紫杉醇治疗A549肺癌的实验研究 [J]．中国中药杂志， 2018 ， 43 ( 7 )： 1446 - 1452 .

华夏，李广兴，刘仲涛，等．人参皂苷Rh 2 下调Wnt/ β -catenin信号通路调控SHG44细胞的增殖和凋亡 [J]．解剖学研究， 2018 ， 40 ( 3 )： 189 - 192 .

刘小霞，夏菁，唐家锋，等．人参皂苷Rh 2 通过激活p38诱导K562细胞自噬凋亡的实验研究 [J]．中国中药杂志， 2017 ， 42 ( 1 )： 146 - 151 .

钱颖，黄容容，孙锐，等．人参皂苷Rh 2 对免疫低下小鼠的免疫调节作用 [J]．医药导报， 2018 ， 37 ( 12 )： 1446 - 1454 .

贾执瑛，谢燮，王晓艳，等．人参主要成分对大鼠免疫功能的比较研究 [J]．中国中药杂志， 2014 ， 39 ( 17 )： 3363 - 3366 .

陈金鸾．不同产区人参中营养成分及有效成分的研究 [D]．长春：吉林大学， 2016 .

肖新月，尹继飞，张南平，等．不同生长年限的人参中8种主要皂苷成分的分析研究 [J]．药物分析杂志， 2004 ， 24 ( 3 )： 238 - 244 .

余江锋，李育平，何伟，等．吉产不同生长年限人参中8种主要人参皂苷与人参皂苷Rg 1 比值的变化规律研究 [J]．中国药房， 2019 ， 30 ( 1 )： 31 - 35 .

曲桂武，夏学超，潘丽丽，等 . 8种人参皂苷在人参全草中的分布情况 [J]．中国实验方剂学杂志， 2015 ， 21 ( 17 )： 52 - 55 .

邢楠楠，赵雨，刘宏，等．不同产地、不同年限人参中淀粉酶、酯酶、酸性磷酸酯酶的活力比较 [J]．中国现代应用药学， 2011 ， 28 ( 1 )： 44 - 47 .

白雪媛，赵雨，刘海龙，等．不同产地人参中的总糖、还原糖和可溶性多糖含量的比较研究 [J]．中国现代应用药学， 2012 ， 29 ( 1 )： 39 - 42 .

M SYLVAIN , S SHANE ．利用LC/Q-TOF和Mass Profiler Professional软件筛查水中新兴化学污染物 [J]．环境化学， 2016 ， 35 ( 3 )： 597 - 600 .

Y WANG , S ZHOU , M WANG , et al . RRLC/Q-TOFMS-based metabolomics for the characterization of cold and hot properties of Chinese Materia Medica [J]． J Ethnopharmacol ， 2016 ， 179 ： 234 - 242 .

L LI , Y WANG , Y XIU , et al . Chemical differentiation and quantitative analysis of different types of Panax genus stem-leaf based on a UPLC-Q-exactive orbitrap/MS combined with multivariate statistical analysis approach [J]． J Anal Methods Chem ， 2018 ， doi： 10.1155/2018/9598672 http://dx.doi.org/10.1155/2018/9598672 .

L S RANDAL , P TOM , D BRIAN ． Perl语言入门 [M]．南京：东南大学出版社， 2009 .

B MERTENS , M THOMPSON , T FEARN ． Principal component outlier detection and SIMCA: a synthesis [J]． Analyst ， 1994 ， 119 ( 12 )： 2777 - 2784 .

Y XIU , X LI , D XIAO , et al . Simultaneous determination and difference evaluation of 14 ginsenosides in Panax ginseng roots cultivated in different areas and ages by high-performance liquid chromatography coupled with triple quadrupole mass spectrometer in the multiple reaction-monitoring mode combined with multivariate statistical analysis [J]． J Ginseng Res ， 2017 ， 43 ( 4 )： 508 - 516 .

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Differences in Intestinal Absorption Characteristics of Nanophase in Single and Combined Decoctions of Ginseng Radix et Rhizoma and Zingiberis Rhizoma Recens Based on Everted Gut Sac Model

Exploring Effect of Concentration on Quantitative Accuracy of QAMS by Taking Ginsenosides as an Example

Analysis on Determination and Quantity Transfer of Standard Decoction of Ginseng Radix et Rhizoma by Fresh and Traditional Cutting

Anti-aging Effect of Ginseng Radix et Rhizoma：A Review

Related Author

GUO Xiaomeng

WANG Qi

LI Meijing

ZHANG Nan

GONG Muxin

CHEN Xi

KONG Shasha

DAI Yuntao

Related Institution

Beijing Key Laboratory of Traditional Chinese Medicine（TCM） Collateral Disease Theory Research，School of TCM，Capital Medical University

Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences

The 3rd Affiliated Hospital of Changchun University of Chinese Medicine

School of Pharmaceutical Sciences， Changchun University of Chinese Medicine

National Key Laboratory for Innovation and Transformation of Luobing Theory

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰