Study on Inhibitory Effect of Huayu Qutan Recipe on Myocardial Fibrosis in Atherosclerotic Rabbits Based on Mitochondrial Fusion-lysis

您当前的位置：

首页 >

文章列表页 >

Study on Inhibitory Effect of Huayu Qutan Recipe on Myocardial Fibrosis in Atherosclerotic Rabbits Based on Mitochondrial Fusion-lysis

Pharmacology | 更新时间：2021-02-09

- Study on Inhibitory Effect of Huayu Qutan Recipe on Myocardial Fibrosis in Atherosclerotic Rabbits Based on Mitochondrial Fusion-lysis
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 26, Issue 3, Pages: 71-77(2020)
- 作者机构：
  
  辽宁中医药大学，沈阳　 110032
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20200340
  CLC： R22; R24; R289; R285
- Received：18 May 2019，
  
  Published Online：18 October 2019，
  
  Published：05 February 2020
- 稿件说明：
移动端阅览
Qu ZHENG, Lian-qun JIA, Nan SONG, et al. Study on Inhibitory Effect of Huayu Qutan Recipe on Myocardial Fibrosis in Atherosclerotic Rabbits Based on Mitochondrial Fusion-lysis[J]. Chinese journal of experimental traditional medical formulae, 2020, 26(3): 71-77.
DOI：

Qu ZHENG, Lian-qun JIA, Nan SONG, et al. Study on Inhibitory Effect of Huayu Qutan Recipe on Myocardial Fibrosis in Atherosclerotic Rabbits Based on Mitochondrial Fusion-lysis[J]. Chinese journal of experimental traditional medical formulae, 2020, 26(3): 71-77. DOI： 10.13422/j.cnki.syfjx.20200340.

摘要

目的：

2

探讨化瘀祛痰方通过影响线粒体融合-裂解对动脉粥样硬化家兔心肌纤维化的抑制作用。

方法：

2

选取SPF级健康雄性家兔36只，随机选取6只为正常组，给予普通颗粒饲料；另30只采用高脂饲料建立动脉粥样硬化模型。模型复制成功后，随机分为模型组、化瘀祛痰方低、中、高剂量组(4.0，8.0，16.0 g·kg

-1

)及辛伐他汀组(1.4 mg·kg

-1

)，各6只。各给药组按剂量给予相应药物，连续给药4周。全自动生物化学分析仪检测各组家兔血清血脂水平，马松(Masson)染色法观察各组家兔心肌纤维化程度，免疫组化法检测心肌组织线粒体融合蛋白1(Mitofusin1)，线粒体融合蛋白2(Mitofusin2)，视神经萎缩蛋白1(Opa1)，发动蛋白相关蛋白1(Drp1)，分裂蛋白1(Fis1)表达水平。

结果：

2

与正常组比较，模型组家兔血清总胆固醇(TC)，甘油三酯(TG)，低密度脂蛋白胆固醇(LDL-C)水平明显升高，高密度脂蛋白胆固醇(HDL-C)水平明显降低，心肌组织Mitofusin1，Mitofusin2，Opa1表达水平明显降低，Drp1，Fis1表达水平明显升高(

P

<

0.05，

P

<

0.01)，与模型组比较，辛伐他汀组及化瘀祛痰方低、中、高剂量组家兔血清TC，TG，LDL-C水平明显降低，HDL-C水平明显升高，心肌组织Mitofusin1，Mitofusin2，Opa1表达水平明显升高，Drp1，Fis1表达水平明显降低(

P

<

0.05，

P

<

0.01)，与化瘀祛痰方高剂量组比较，辛伐他汀组及化瘀祛痰方低、中剂量组血清TC，TG，LDL-C水平明显升高，HDL-C水平明显降低，心肌组织Mitofusin1，Mitofusin2，Opa1表达水平明显降低，Drp1，Fis1表达水平明显升高(

P

<

0.05，

P

<

0.01)。

结论：

2

化瘀祛痰方能有效调节血脂，抑制粥样硬化家兔心肌纤维化，且化瘀祛痰方剂量越高效果越明显，推测其作用可能与调节心肌细胞线粒体融合-裂解相关蛋白Mitofusin1，Mitofusin2及Opa1，Drp1，Fis1表达有关。

Abstract

Objective:

2

To analysis the effect of Huayu Qutan recipe on myocardial fibrosis in atherosclerotic rabbits based on mitochondrial fusion-lysis.

Method:

2

The 36 SPF healthy male rabbits were selected

and 6 rabbits were selected randomly as the normal group

and given normal pellet feed

another 30 rats were fed with high fat diet to establish atherosclerosis model.After successful replication of animal models

they were randomly divided into model group

Huayu Qutan recipe low-dose

medium-dose and high-dose group (4.0

8.0

16.0 g·kg

-1

) and simvastatin group (1.4 mg·kg

-1

)

6 rats each.Each group was given corresponding drugs according to the dosage

continuous administration for 4 weeks.The serum lipid levels in rabbits of each group were detectived by automatic biochemical analyzer

the degree of myocardial fibrosis was measured by Masson staining

and expression levels of mitochondrial fusion protein 1 (Mitofusin 1)

mitochondrial fusion protein 2 (Mitofusin 2)

optic atrophy protein 1 (Opa1)

promoter protein 1 (Drp1)

mitogen 1 (Fis1) in myocardial tissue were detected by immunohistochemistry.

Result:

2

Compared with normal group

the levels of total cholesterol (TC)

triglyceride (TG)

low density lipoprotein cholesterol (LDL-C) in model group were significantly increased

levels of density lipoprotein cholesterol (HDL-C) were significantly decreased

the expression levels of Mitofusin 1

Mitofusin 2 and Opa1 in myocardial tissue were significantly decreased

the expression levels of Drp1 and Fis1 were significantly increased(

P

<

0.05

P

<

0.01)

compared with model group

the levels of serum TC

TG

LDL-C in simvastatin group and Huayu Qutan recipe low-dose

medium and high-dose group were significantly decreased

levels of HDL-C were significantly increased

the expression levels of Mitofusin 1

Mitofusin 2 and Opa1 in myocardial tissue were significantly increased

the expression levels of Drp1 and Fis1 were significantly decreased (

P

<

0.05

P

<

0.01)

compared with the Huayu Qutan recipe high-dose group

the levels of serum TC

TG

LDL-C in simvastatin group and Huayu Qutan recipe low

medium-dose group were significantly increased

the expression levels of Mitofusin 1

Mitofusin 2 and Opa1 in myocardial tissue were significantly decreased

the expression levels of Drp1 and Fis1 were significantly increased(

P

<

0.05

P

<

0.01).

Conclusion:

2

Huayu Qutan recipe can effectively regulate blood lipid and inhibit myocardial fibrosis in atherosclerotic rabbits

and the higher the dose of Huayu Qutan recipe

the more obvious the effect is

it is speculated that its effect may be related to the regulation of the expression of mitochondrial fusion-lysis related proteins such as Mitofusin1

Mitofusin2 and Opa1

Drp1 and Fis1 in myocardial cells.

关键词

Keywords

references

A L Jonsson ， F Bäckhed ． Role of gut microbiota in atherosclerosis [J]． Nat Rev Cardiol ， 2017 ， 14 ( 2 )： 79 .

底彬，鹿育萨，张国江．兔动脉粥样硬化对心脏胶原的影响及培哚普利的干预 [J]．实用医技杂志， 2016 ， 23 ( 11 )： 1164 - 1166，1265 .

L J Worke ， J E Barthold ， B Seelbinder , et al . Densification of type I collagen matrices as a model for cardiac fibrosis [J]． Adv Healthc Mater ， 2017 ， 6 ( 22 )： 1700114 .

V Papademetriou ， N Katsiki ， M Doumas ， et al . Halting arterial aging in patients with cardiovascular disease: hypolipidemic and antihypertensive therapy [J]． Curr Pharm Des ， 2014 ， 20 ( 40 )： 6339 - 6349 .

蔡倩玮，于远望．中医药防治心肌纤维化的实验研究进展 [J]．中医药导报， 2017 ， 23 ( 1 )： 76 - 79 .

陈凯，刘馨，管怡晴，等．基于网络药理学构建新型中药复方降压抗纤方抗高血压心肌纤维化的作用 [J]．中国实验方剂学杂志， 2018 ， 24 ( 20 )： 115 - 122 .

孙艳梅，贾连群，张妮，等．化瘀祛痰方调控AMPK介导自噬对高脂血症大鼠肝脏脂质损伤的影响 [J]．中国煤炭工业医学杂志， 2017 ， 20 ( 3 )： 312 - 316 .

陈丝，贾连群，宋囡，等．化瘀祛痰方调控线粒体自噬相关蛋白减少动脉粥样硬化家兔肝脏脂质沉积的研究 [J]．北京中医药大学学报， 2018 ， 41 ( 4 )： 310 - 315 .

陈宁，宋囡，贾连群，等．化瘀祛痰方对AS家兔肝脏线粒体呼吸链酶复合物的影响 [J]．中国实验方剂学杂志， 2018 ， 24 ( 7 )： 171 - 176 .

贾连群，杨关林，任路，等．化瘀祛痰方药及其拆方对H 2 O 2 诱导EA.hy926细胞凋亡的影响及机制研究 [J]．中药新药与临床药理， 2012 ， 23 ( 1 )： 5 - 10 .

于洁，高传玉，许文克，等．超声检查和血管造影对兔动脉粥样硬化狭窄模型的评价 [J]．中国临床康复， 2006 ， 10 ( 40 )： 51 - 53，193 .

D Y HU ． New guidelines and evidence for prevention and treatment of dyslipidemia and atherosclerotic cardiovascular disease in China [J]． CDTM ， 2016 ， 44 ( 10 )： 826 .

B F Bale ， A L Doneen ， D J Vigerust ． High-risk periodontal pathogens contribute to the pathogenesis of atherosclerosis [J]． Postgrad Med J ， 2017 ， 93 ( 1098 )： 215 - 220 .

R S Wiehe ， B Gole ， L Chatre ， et al . Endonuclease G promotes mitochondrial genome cleavage and replication [J]． PLoS One ， 2018 , 9 ( 26 )： 18309 - 18326 .

J O'Reilly ， F Pangilinan ， K Hokamp ， et al . The impact of common genetic variants in the mitochondrial glycine cleavage system on relevant metabolites [J]． Mol Genet Metab ， 2018 ， 16 ： 20 - 22 .

F YANG ， R WU ， Z JIANG ， et al . Leptin increases mitochondrial OPA1 via GSK3-mediated OMA1 ubiquitination to enhance therapeutic effects of mesenchymal stem cell transplantation [J]． Cell Death Dis ， 2018 ， 9 ( 5 )： 556 .

具星，贾连群，张哲，等．益气健脾化瘀祛痰方对脾虚痰浊动脉粥样硬化巴马小型猪心脏线粒体蛋白质组的影响 [J]．中华中医药杂志， 2017 ， 32 ( 8 )： 3659 - 3662 .

冷雪，李阳，宋囡，等．化瘀祛痰方对ApoE -/- 小鼠主动脉MAPK信号通路相关蛋白表达的影响 [J]．中国实验方剂学杂志， 2019 , 25 ( 4 )： 116 - 121 .

王英，贾连群，宋囡，等．健脾祛痰化瘀方药对脾虚痰浊动脉粥样硬化巴马猪肝脏线粒体能量代谢基因表达的影响 [J]．辽宁中医杂志， 2017 ， 44 ( 11 )： 2427 - 2429 .

T Calì ， D Ottolini ， A Negro ， et al . A-Synuclein controls mitochondrial calcium homeostasis by enhancing endoplasmic reticulum-mitochondria interactions [J]． J Biol Chem ， 2012 ， 287 ( 22 )： 17914 .

K PENG ， Y TAO ， J ZHANG ， et al . Resveratrol regulates mitochondrial biogenesis and fission/fusion to attenuate rotenone-induced neurotoxicity [J]． Oxid Med Cell Longev ， 2016 ， article a259 ： 1 - 12 .

H Kim ， M C Scimia ， D Wilkinson ， et al . Fine-tuning of Drp1/Fis1 availability by AKAP121/Siah2 regulates mitochondrial adaptation to hypoxia [J]． Mol Cell ， 2011 ， 44 ( 4 )： 532 - 544 .

G Chandhok ， M Lazarou ， B Neumann ． Structure, function, and regulation of mitofusin-2 in health and disease [J]． Biol Rev ， 2017 ， 93 ( 2 )： 933 - 949 .

J M Son , E H Sarsour , B A Kakkerla , et al . Mitofusin 1 and optic atrophy 1 shift metabolism to mitochondrial respiration during aging [J]． Aging Cell , 2017 , 16 ( 5 )： 1136 - 1145 .

Q LIU , L KANG , L WANG , et al . Mitofusin 2 regulates the oocytes development and quality by modulating meiosis and mitochondrial function [J]． Sci Rep , 2016 , 6 ( 1 )： 30561 .

0

Views

18

下载量

2

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Related Articles

Effect of Huayu Qutan Recipe and Its Separated Recipes on Inflammation Factors in EA.hy926 Cells Induced by Lipopolysaccharide

Effect of Huayu Qutan Recipe on Respiratory Chain Enzyme Complexes of Liver Mitochondria in AS Rabbits

Exploration of Zhuyuwan in Treatment of Atherosclerosis from Perspective of Lipid Transport Disorder

Mechanism of Huangqi Chifengtang in Treating Atherosclerosis Based on 16S rRNA Sequencing and Metabolomics

Effect of Co-treatment Method of Stagnation of Phlegm and Blood Stasis （Danlou Tablet） on Vascular Endothelial Function in Patients with Atherosclerosis

Related Author

JIA Lian-qun

YANG Guan-lin

REN Lu

FENG Jun-yi

CHEN Wen-na

CAO Yang

ZHANG Lin

CHEN Ning

Related Institution

Chengdu University of Traditional Chinese Medicine（TCM）

Hospital of Chengdu University of TCM

Hubei Minzu University

Institute of TCM， Sichuan Academy of Chinese Medicine Sciences

Institute of Traditional Chinese Medicine， Heilongjiang University of Chinese Medicine

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰